Mouse Soggy-1/DkkL1 Antibody Summary
Accession # AAF02679
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Mouse Soggy‑1/DkkL1 by Western Blot. Western blot shows lysate of mouse testis tissue. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Mouse Soggy-1/DkkL1 Monoclonal Antibody (Catalog # MAB1508) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Soggy-1/DkkL1 at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Soggy-1, also known as DkkL1 (Dickkopf-like 1), is a secreted protein that is related to the Dickkopf family of Wnt antagonists. Dkk-1, -2, -3, and -4 each have two cysteine-rich domains separated by a linker. Soggy-1 does not contain cysteine-rich domains but bears some sequence homology with the N-terminal regions of Dkk proteins (1, 2). The mouse Soggy-1 cDNA encodes a 230 amino acid (aa) precursor that includes a 20 aa signal sequence (2). Mouse Soggy-1 shares 65% and 90% aa sequence identity with human and rat Soggy-1, respectively, and approximately 15% aa sequence identity with mouse Dkk-1, -2, -3, and -4. Mouse Soggy-1 is expressed at various sites in the embryo but in the adult is primarily found in the testes (3‑5). Soggy-1 transcription is regulated by the spermatocyte specific factor, RFX2 (6). The regulatory elements for Soggy-1 lie very close to those of TEAD-2, a transcription factor that is expressed very early in development (4). Soggy-1 and TEAD-2 are co-expressed in preimplantation embryos and embryonic stem cells, but differentiated cells express only one or the other (4, 5). During development, Soggy-1 is first detectable at the onset of sexual differentiation (4). Soggy-1 is localized to the acrosome in developing spermatocytes and mature spermatozoa (3, 4). Soggy-1, as expressed in developing mouse spermatocytes, is a 34 kDa N-glycosylated protein. This glycosylation is not present on Soggy-1 in mature spermatozoa, although the apparent molecular weight suggests the presence of some post-translational modification (3). Two shorter forms of Soggy-1 have been described that result from the use of internal methionine residues for initiation (4).
- Kawano, Y. and R. Kypta (2003) J. Cell Sci. 116:2627.
- Krupnik, V.E. et al. (1999) Gene 238:301.
- Kohn, M.J. et al. (2005) Mol. Reprod. Dev. 71:516.
- Kaneko, K.J. and M.L. DePamphilis (2000) Nucleic Acids Res. 28:3982.
- Kaneko, K.J. et al. (2004) Mol. Cell. Biol. 24:1968.
- Horvath, G.C. et al. (2004) Biol. Reprod. 71:1551.
Citation for Mouse Soggy-1/DkkL1 Antibody
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1 Citation: Showing 1 - 1
DkkL1 (Soggy), a Dickkopf family member, localizes to the acrosome during mammalian spermatogenesis.
Authors: Kohn MJ, Kaneko KJ, DePamphilis ML
Mol. Reprod. Dev., 2005;71(4):516-22.
Sample Types: Tissue Homogenates
Applications: Western Blot
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