Mouse Soggy-1/DkkL1 Antibody

(1 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Soggy-1/DkkL1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human Soggy-1/DkkL1 is observed.
  • Source
    Monoclonal Rat IgG2B Clone # 192425
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse Soggy-1/DkkL1
    Leu21-Leu230
    Accession # AAF02679
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.5 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse Soggy‑1/DkkL1 by Western Blot. Western blot shows lysate of mouse testis tissue. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Mouse Soggy‑1/DkkL1 Monoclonal Antibody (Catalog # MAB1508) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Soggy‑1/DkkL1 at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Soggy-1/DkkL1

Soggy-1, also known as DkkL1 (Dickkopf-like 1), is a secreted protein that is related to the Dickkopf family of Wnt antagonists. Dkk-1, -2, -3, and -4 each have two cysteine-rich domains separated by a linker. Soggy-1 does not contain cysteine-rich domains but bears some sequence homology with the N-terminal regions of Dkk proteins (1, 2). The mouse Soggy-1 cDNA encodes a 230 amino acid (aa) precursor that includes a 20 aa signal sequence (2). Mouse Soggy-1 shares 65% and 90% aa sequence identity with human and rat Soggy-1, respectively, and approximately 15% aa sequence identity with mouse Dkk-1, -2, -3, and -4. Mouse Soggy-1 is expressed at various sites in the embryo but in the adult is primarily found in the testes (3‑5). Soggy-1 transcription is regulated by the spermatocyte specific factor, RFX2 (6). The regulatory elements for Soggy-1 lie very close to those of TEAD-2, a transcription factor that is expressed very early in development (4). Soggy-1 and TEAD-2 are co-expressed in preimplantation embryos and embryonic stem cells, but differentiated cells express only one or the other (4, 5). During development, Soggy-1 is first detectable at the onset of sexual differentiation (4). Soggy-1 is localized to the acrosome in developing spermatocytes and mature spermatozoa (3, 4). Soggy-1, as expressed in developing mouse spermatocytes, is a 34 kDa N-glycosylated protein. This glycosylation is not present on Soggy-1 in mature spermatozoa, although the apparent molecular weight suggests the presence of some post-translational modification (3). Two shorter forms of Soggy-1 have been described that result from the use of internal methionine residues for initiation (4).

  • References:
    1. Kawano, Y. and R. Kypta (2003) J. Cell Sci. 116:2627.
    2. Krupnik, V.E. et al. (1999) Gene 238:301.
    3. Kohn, M.J. et al. (2005) Mol. Reprod. Dev. 71:516.
    4. Kaneko, K.J. and M.L. DePamphilis (2000) Nucleic Acids Res. 28:3982.
    5. Kaneko, K.J. et al. (2004) Mol. Cell. Biol. 24:1968.
    6. Horvath, G.C. et al. (2004) Biol. Reprod. 71:1551.
  • Long Name:
    Dickkopf-like Protein 1
  • Entrez Gene IDs:
    27120 (Human); 50722 (Mouse)
  • Alternate Names:
    Cancer/testis antigen 34; CT34dickkopf-like 1 (soggy); dickkopf-like 1; DkkL1; Protein soggy-1; SGY; SGY1; SGY-1dickkopf-like protein 1; SGY1soggy; Soggy1; Soggy-1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. DkkL1 (Soggy), a Dickkopf family member, localizes to the acrosome during mammalian spermatogenesis.
    Authors: Kohn MJ, Kaneko KJ, DePamphilis ML
    Mol. Reprod. Dev., 2005;71(4):516-22.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
Isotype Controls
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Rat IgG2B Isotype Control

Ctrl MAB0061 27  
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Normal Rat IgG Control

Ctrl 6-001-A 9
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Normal Rat IgG Control (Azide Free)

Ctrl 6-001-F 1
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Rat IgG2B Isotype Control

Ctrl MAB0061R
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Secondary Antibodies
Description Application Cat# Citations Images  

Rat IgG HRP-conjugated Antibody

WB, Simple Western HAF005 7  
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Goat Anti-Rat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL013 2
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Goat Anti-Rat F(ab)2 IgG (H+L)
APC-conjugated Antibody

Flow F0113 2  
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Rat F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0105B 2  
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Goat Anti-Rat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL015 1
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Goat Anti-Rat IgG Biotinylated Antibody

WB BAF005 2
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Rat IgG VisUCyte HRP Polymer Antibody

IHC VC005  
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Goat Anti-Rat IgG Antibody

WB AF005 1
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Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0104B  
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Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL014
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Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0115 1
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