Solid Phase Sandwich ELISA
96-well strip plate
7.81 - 500 pg/mL (Cell Culture Supernates, Serum, Heparin Plasma,)
Natural and recombinant mouse Shh-N. This kit also recognizes low levels of Shh-N in rat serum.
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
No significant interference observed with available related molecules.
The Quantikine Mouse Sonic Hedgehog N-Terminus Immunoassay is a 4.5 hour solid phase ELISA designed to measure Shh-N in cell culture supernates, serum, and plasma. It contains recombinant mouse Shh-N and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant factor accurately. Results obtained using natural mouse Shh-N showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse Shh-N.
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.Cell Culture Supernates, Serum, Heparin Plasma
|Standard Deviation||1.08||1.31||10.6||2.28||2.96||9.21 |
The recovery of mouse Shh-N spiked to levels throughout the range of the assay in various matrices was evaluated.
|Sample Type ||Average % Recovery ||Range % |
|Cell Culture Samples (n=6) ||101 ||96-109 |
|Heparin Plasma (n=4) ||97 ||86-108 |
|Serum (n=4) ||98 ||86-109 |
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse Shh-N were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Preparation and Storage
Background: Sonic Hedgehog/Shh
Shh (Sonic Hedgehog) is expressed in embryonic tissues that are critical for the patterning of the developing central nervous system, somite, and limb. It is also involved in whisker, hair, foregut, tooth, and bone development. Shh regulates neural and hematopoietic stem cell fate and is important for thymocyte differentiation and proliferation. In adult tissue Shh is associated with cancer development and tissue remodeling following injury. Palmitoylation and cholesterol addition of Shh contribute to its membrane tethering and assembly into multimers. Lipid modification and multimerization greatly increase Shh signaling potency. Shh can be released from the plasma membrane by the cooperative action of DISP1, SCUBE2, and TACE/ADAM17. Canonical signaling of Shh is mediated by a receptor complex that includes Patched (PTCH1, PTCH2) and Smoothened (SMO).
Related Research Areas
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