Mouse SOST/Sclerostin Antibody

Catalog # Availability Size / Price Qty
MAB1589
MAB1589-SP
Product Details
Citations (3)
FAQs
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Mouse SOST/Sclerostin Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse SOST in direct ELISAs and Western blots. In direct ELISAs, this antibody does not cross-react with recombinant human SOST.
Source
Monoclonal Rat IgG2B Clone # 248121
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse SOST
Gln24-Tyr211
Accession # AAI37834
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse SOST/Sclerostin (Catalog # 1589-ST)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SOST/Sclerostin

SOST, also known as sclerostin, is a member of the cerberus/DAN family, a group of secreted glycoproteins characterized by a cysteine-knot motif. Cerberus/DAN family members are putative BMP antagonists, and include Dan, Cerberus, Gremlin, PRDC, and Caronte. While the overall sequence identity between members of the family is low, they have conserved spacing of six cysteine residues. Cerberus and Dan have an additional cysteine residue used for dimerization; however, SOST does not and is secreted as a monomer. SOST was originally identified as an important regulator of bone homeostasis. Positional cloning studies identified that mutations in the SOST gene can cause sclerosteosis and van Buchem disease, bone dysplasia disorders characterized by progressive skeletal overgrowth. Significant levels of SOST expression are detected in bone, cartilage, kidney, and liver. SOST is expressed by osteoclasts in developing bones of mouse embryos, including both intramembranously forming skull bones and endochondrally forming long bones. SOST plays a physiological role as a negative regulator of bone formation by repressing BMP-induced osteogenesis. SOST has been shown to have unique ligand specificity, binding BMP-5, -6, and -7 with high affinity and BMP-2 and -4 with low affinity. This seems to be the first example of a BMP antagonist being localized to osteoclasts, cells derived from the hematopoietic lineage, that function to degrade bone matrix. Recombinant human SOST preparations from R&D Systems bind BMP-5 and BMP-6 in a functional ELISA. Human and mouse SOST share 88% amino acid identity (1-3).

References
  1. Kusu, N. et al. (2003) J. Biol. Chem. 278:24113.
  2. Balemans, W. et al. (2001) Hum. Mol. Genet. 10:537.
  3. Brunkow, M.E. et al. (2001) Am. J. Hum. Genet. 68:577.
Entrez Gene IDs
50964 (Human); 74499 (Mouse)
Alternate Names
sclerostin; SOST; VBCHsclerosteosis

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Citations for Mouse SOST/Sclerostin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Osteocyte CIITA aggravates osteolytic bone lesions in myeloma
    Authors: H Liu, J He, R Bagheri-Ya, Z Li, R Liu, Z Wang, DH Bach, YH Huang, P Lin, TA Guise, RF Gagel, J Yang
    Nature Communications, 2022-06-27;13(1):3684.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Mechanical stimulation of bone in vivo reduces osteocyte expression of Sost/sclerostin.
    Authors: Robling AG, Niziolek PJ, Baldridge LA, Condon KW, Allen MR, Alam I, Mantila SM, Gluhak-Heinrich J, Bellido TM, Harris SE, Turner CH
    J. Biol. Chem., 2007-12-17;283(9):5866-75.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. A crucial role for matrix metalloproteinase 2 in osteocytic canalicular formation and bone metabolism.
    Authors: Inoue K, Mikuni-Takagaki Y, Oikawa K, Itoh T, Inada M, Noguchi T, Park JS, Onodera T, Krane SM, Noda M, Itohara S
    J. Biol. Chem., 2006-09-07;281(44):33814-24.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P

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