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Mouse SP-D Quantikine ELISA Kit

R&D Systems | Catalog # MSFPD0

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Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 µL), Tissue Lysates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL)

Sensitivity

9.43 pg/mL

Assay Range

62.5-4000 pg/mL (Cell Culture Supernates, Tissue Lysates, Serum, EDTA Plasma, Heparin Plasma)
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Product Summary for Mouse SP-D Quantikine ELISA Kit

The Quantikine Mouse SP-D immunoassay is a 4.5 hour solid-phase ELISA designed to measure SP-D in mouse cell culture supernates, tissue lysates, serum, and plasma. It contains CHO cell-expressed recombinant mouse SP-D and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant mouse SP-D. Results obtained using natural mouse SP-D showed dose response curves that were parallel to the standard curves obtained using the Quantikine mouse kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse SP-D.

Product Specifications

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Species

Mouse

Specificity

Natural and recombinant mouse SP-D.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of kit components.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum, Tissue Lysates

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 4 4 4 4 4 4
Mean (pg/mL) 158 534 1270 169 531 1267
Standard Deviation 5.75 13.8 43.0 14.0 34.4 120
CV% 3.6 2.6 3.4 8.3 6.5 9.5

Recovery for Mouse SP-D Quantikine ELISA Kit

The recovery of mouse SP-D spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 99 92-105
EDTA Plasma (n=4) 97 90-103
Heparin Plasma (n=4) 97 84-104
Serum (n=4) 96 84-109
Tissue Lysates (n=4) 101 91-113

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse SP-D in each matrix were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay

Mouse SP-D ELISA Linearity

Scientific Data Images for Mouse SP-D Quantikine ELISA Kit

Mouse SP-D ELISA Standard Curve

Mouse SP-D ELISA Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: SP-D

Surfactant Protein D (SP-D) is a collectin family protein consisting of a collagen-like sequence followed by a C-type lectin domain. It is synthesized primarily in the lung by alveolar type II cells and unciliated bronchial epithelial cells. In addition, SP-D is produced by epithelial cells of the gastrointestinal tract and exocrine glands. SP-D binds to patterns of neutral sugars on micro-organisms to mediate direct opsonization and phagocytosis and is an important component of the innate immune system.

Long Name

Surfactant Pulmonary Associated Protein D

Alternate Names

Collectin 7, PSP-D, SFTP4, SFTPD, SPD

Entrez Gene IDs

6441 (Human); 20390 (Mouse)

Gene Symbol

SFTPD

Additional SP-D Products

Product Documents for Mouse SP-D Quantikine ELISA Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse SP-D Quantikine ELISA Kit

For research use only

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Mouse SP-D Quantikine ELISA Kit

Customer Reviews for Mouse SP-D Quantikine ELISA Kit (2)

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  • Mouse SP-D Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Serum
    Verified Customer | Posted 02/18/2021
    Mouse SP-D Quantikine ELISA Kit MSFPD0
  • Mouse SP-D Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: FVBN mouse plasma and BAL
    Verified Customer | Posted 03/06/2019
    I used this kit to analyze SP-D of FVBN mice plasma and BAL infected with 0111:B4 LPS. I had a lot of background signal which made it hard to distinguish the difference of SP-D between control and infected mice. I think the kit itself is good and easy to use though, I just need to increase the difference to compensate for background noise. I also didn't dilute enough in my BAL, so my std curve was not allowed to develop as much as I would have liked. If you run with BAL I would recommend a 1:500 dilution! I did 1:200.
    Mouse SP-D Quantikine ELISA Kit MSFPD0

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Protocols

View specific protocols for Mouse SP-D Quantikine ELISA Kit (MSFPD0):

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 50 µL Assay Diluent
  3.   Add 50 µL of Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  5.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
    6. 100 µL Conjugate
  6.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
  7.   Aspirate and wash 4 times.
    8. 100 µL Substrate Solution
  8.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
    9. 100 µL Stop Solution
  9.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

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FAQs

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