P-Selectin, also known as CD62P, GMP-140, PADGEM, and LECAM-3, is a transmembrane adhesion protein that is upregulated on activated platelets and vascular endothelial cells. It binds to hematopoietic cell PSGL-1/CD162 with highly clustered O-linked sialyl Lewis-x (sLex ) tetrasaccharides and sulfated tyrosine, as well as to heparin sulfate, Versican, and CD42b/GPIb alpha. P-Selectin mediates the initial interaction of circulating leukocytes with activated endothelial cells that produces a characteristic ‘rolling’ of the leukocytes on the endothelium. It is therefore critical for leukocyte extravasation at sites of inflammation and leukocyte involvement in thrombus formation at sites of vascular injury.
Mouse sP-Selectin/CD62P Quantikine ELISA Kit
R&D Systems | Catalog # MPS00
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Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (10 µL), EDTA Plasma (10 µL)
Sensitivity
0.018 ng/mL
Assay Range
0.31-20 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma)
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Product Summary for Mouse sP-Selectin/CD62P Quantikine ELISA Kit
The Quantikine Mouse soluble P-Selectin (sP-Selectin) Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse sP-Selectin levels in cell culture supernates, serum, and EDTA plasma. It contains NS0-expressed recombinant mouse sP-Selectin and antibodies raised against the recombinant factor. Results obtained for naturally occurring mouse sP-Selectin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values of natural mouse sP-Selectin.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Species
Mouse
Specificity
Natural and recombinant mouse sP-Selectin
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 32 | 36 | 30 |
| Mean (ng/mL) | 1.27 | 2.92 | 12.9 | 1.27 | 2.90 | 12.6 |
| Standard Deviation | 0.10 | 0.23 | 1.13 | 0.08 | 0.24 | 1.13 |
| CV% | 7.9 | 7.9 | 8.8 | 6.3 | 8.3 | 9.0 |
Recovery for Mouse sP-Selectin/CD62P Quantikine ELISA Kit
The recovery of mouse sP-Selectin spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=4) | 100 | 89-110 |
| EDTA Plasma (n=6) | 94 | 80-112 |
| Serum (n=10) | 98 | 81-115 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse sP-Selectin in each matrix were diluted with Calibrator Diluent and then assayed.
Scientific Data Images for Mouse sP-Selectin/CD62P Quantikine ELISA Kit
Mouse P-Selectin/CD62P ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: P-Selectin/CD62P
Alternate Names
CD62P, GMP140, GRMP, PADGEM, PSEL, SELP
Gene Symbol
SELP
Additional P-Selectin/CD62P Products
Product Documents for Mouse sP-Selectin/CD62P Quantikine ELISA Kit
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse sP-Selectin/CD62P Quantikine ELISA Kit
For research use only
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.Citations for Mouse sP-Selectin/CD62P Quantikine ELISA Kit
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Sample Tested: Citrate PlasmaVerified Customer | Posted 10/12/2021We diluted the plasma 1:25
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Sample Tested: Serum and PlasmaVerified Customer | Posted 09/14/2019
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Protocols
View specific protocols for Mouse sP-Selectin/CD62P Quantikine ELISA Kit (MPS00):
Refer to the product for complete assay procedure.
Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 50 µL of Assay Diluent to each well.
- Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
- Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.
- Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
- Aspirate and wash 5 times.
- Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
- Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
50 µL Assay Diluent
50 µL Standard, Control, or Sample
100 µL Conjugate
100 µL Substrate Solution
100 µL Stop Solution
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