Mouse sP-Selectin/CD62P Quantikine ELISA Kit

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MPS00
Mouse P-Selectin/CD62P ELISA Standard Curve
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Product Details
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Citations (21)
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Mouse sP-Selectin/CD62P Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL)
Sensitivity
0.018 ng/mL
Assay Range
0.3 - 20 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma)
Specificity
Natural and recombinant mouse sP-Selectin
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Mouse soluble P-Selectin (sP-Selectin) Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse sP-Selectin levels in cell culture supernates, serum, and EDTA plasma. It contains NS0-expressed recombinant mouse sP-Selectin and antibodies raised against the recombinant factor. Results obtained for naturally occurring mouse sP-Selectin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values of natural mouse sP-Selectin.

Recovery

The recovery of mouse sP-Selectin spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=4) 100 89-110
EDTA Plasma (n=6) 94 80-112
Serum (n=10) 98 81-115

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse sP-Selectin in each matrix were diluted with Calibrator Diluent and then assayed.
Mouse P-Selectin/CD62P ELISA Linearity

Scientific Data

Mouse P-Selectin/CD62P ELISA Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: P-Selectin/CD62P

The Selectin family is comprised of three members, E-Selectin, L-Selectin, and P-Selectin. E-Selectin [endothelial leukocyte adhesion molecule-1 (ELAM-1), CD62E] is transiently expressed on vascular endothelial cells in response to IL-1 beta and TNF-alpha. The human and rat proteins share approximately 67% amino acid sequence identity. The mouse and rat proteins share approximately 78% amino acid sequence identity. L-Selectin (Leukocyte Selectin, LAM-1, LECAM-1, LECCAM-1, TQ1, Leu-8, MEL-14 antigen, DREG, lymph node homing receptor, CD62L) is expressed constitutively on a wide variety of leukocytes. Two forms of L-Selectin have been reported, apparently arising as a result of post-translational modifications. Human and mouse L-Selectin share 76% amino acid sequence identity. Human P-Selectin (GMP-140, LECAM-3, PADGEM, CD62P) is expressed by activated platelets and endothelial cells. The extracellular domains of human and mouse P-Selectin share approximately 73% amino acid sequence identity.

Entrez Gene IDs:
6403 (Human); 20344 (Mouse)
Alternate Names:
CD62P antigen; CD62P; FLJ45155; GMP140; GRMP; PADGEM; PADGEMantigen CD62); PSEL; P-Selectin; selectin P (granule membrane protein 140kDa, antigen CD62); SELP
&#9888; WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 50 µL Assay Diluent
  4.   Add 50 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.

  8. 100 µL Conjugate
  9.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  10.   Aspirate and wash 5 times.

  11. 100 µL Substrate Solution
  12.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

  13. 100 µL Stop Solution
  14.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Mouse sP-Selectin/CD62P Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

21 Citations: Showing 1 - 10
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  1. Pre-Administration of PLX-R18 Cells Protects Mice from Radiation-Induced Hematopoietic Failure and Lethality
    Authors: VP Kumar, S Biswas, GP Holmes-Ham, M Sheleg, S Stone, B Legesse, R Ofir, SP Ghosh
    Genes, 2022-09-28;13(10):.
    Species: Mouse
    Sample Types: Serum
  2. Octreotide ameliorates hepatic ischemia-reperfusion injury through SNHG12/TAF15-mediated Sirt1 stabilization and YAP1 transcription
    Authors: SF Zou, YH Peng, CM Zheng, YX Fei, SW Zhao, HP Sun, JF Yang
    Toxicology and Applied Pharmacology, 2022-03-18;442(0):115975.
    Species: Mouse
    Sample Types: Serum
  3. Quercetin Completely Ameliorates Hypoxia-Reoxygenation-Induced Pathophysiology Severity in NY1DD Transgenic Sickle Mice: Intrinsic Mild Steady State Pathophysiology of the Disease in NY1DD Is Also Reversed
    Authors: S Thangaswam, CA Branch, K Ambadipudi, SA Acharya
    Biomolecules, 2021-10-06;11(10):.
    Species: Mouse
    Sample Types: Plasma
  4. Interleukin-1 receptor inhibition reduces stroke size in a murine model of sickle cell disease
    Authors: J Venugopal, J Wang, J Mawri, C Guo, D Eitzman
    Haematologica, 2021-09-01;0(0):.
    Species: Mouse
    Sample Types: Plasma
  5. Blocking connexin43 hemichannels protects mice against tumour necrosis factor-induced inflammatory shock
    Authors: T Delvaeye, MAJ De Smet, S Verwaerde, E Decrock, A Czekaj, RE Vandenbrou, K Lemeire, A Gonçalves, W Declercq, P Vandenabee, DV Krysko, L Leybaert
    Sci Rep, 2019-11-12;9(1):16623.
    Species: Mouse
    Sample Types: Plasma
  6. Sirt3 deficiency does not affect venous thrombosis or NETosis despite mild elevation of intracellular ROS in platelets and neutrophils in mice
    Authors: H Hayashi, D Cherpokova, K Martinod, T Witsch, SL Wong, M Gallant, SM Cifuni, LP Guarente, DD Wagner
    PLoS ONE, 2017-12-13;12(12):e0188341.
    Species: Mouse
    Sample Types: Plasma
  7. Molecular magnetic resonance imaging discloses endothelial activation after transient ischaemic attack
    Authors: Aurélien Quenault
    Brain, 2016-11-08;140(0):146-157.
    Species: Mouse
    Sample Types: Plasma
  8. Milano summer particulate matter (PM10) triggers lung inflammation and extra pulmonary adverse events in mice.
    Authors: Farina F, Sancini G, Battaglia C, Tinaglia V, Mantecca P, Camatini M, Palestini P
    PLoS ONE, 2013-02-25;8(2):e56636.
    Species: Mouse
    Sample Types: Plasma
  9. Early protection from burn-induced acute lung injury by deletion of preprotachykinin-A gene.
    Authors: Sio SW, Moochhala S, Lu J
    Am. J. Respir. Crit. Care Med., 2009-10-01;181(1):36-46.
    Species: Mouse
    Sample Types: Tissue Homogenates
  10. The effects of NMDA receptor antagonists over intestinal ischemia/reperfusion injury in rats.
    Authors: Camara-Lemarroy CR, Guzman-de la Garza FJ, Alarcon-Galvan G, Cordero-Perez P, Fernandez-Garza NE
    Eur. J. Pharmacol., 2009-09-13;621(1):78-85.
    Species: Rat
    Sample Types: Serum
  11. Attenuation of transplant arteriosclerosis with clopidogrel is associated with a reduction of infiltrating dendritic cells and macrophages in murine aortic allografts.
    Authors: Abele S, Spriewald BM, Ramsperger-Gleixner M, Wollin M, Hiemann NE, Nieswandt B, Weyand M, Ensminger SM
    Transplantation, 2009-01-27;87(2):207-16.
    Species: Mouse
    Sample Types: Serum
  12. Biodistribution and safety profile of recombinant adeno-associated virus serotype 6 vectors following intravenous delivery.
    Authors: Stone D, Liu Y, Li ZY, Strauss R, Finn EE, Allen JM, Chamberlain JS, Lieber A
    J. Virol., 2008-05-14;82(15):7711-5.
    Species: Mouse
    Sample Types: Serum
  13. Vascular dysfunction in a murine model of severe hemolysis.
    Authors: Frei AC, Guo Y, Jones DW, Pritchard KA, Fagan KA, Hogg N, Wandersee NJ
    Blood, 2008-05-13;112(2):398-405.
    Species: Mouse
    Sample Types: Plasma
  14. High-dose erythropoietin alters platelet reactivity and bleeding time in rodents in contrast to the neuroprotective variant carbamyl-erythropoietin (CEPO).
    Authors: Kirkeby A, Torup L, Bochsen L, Kjalke M, Abel K, Theilgaard-Monch K, Johansson PI, Bjorn SE, Gerwien J, Leist M
    Thromb. Haemost., 2008-04-01;99(4):720-8.
    Species: Mouse
    Sample Types: Plasma
  15. Reduced plaque formation induced by rosiglitazone in an STZ-diabetes mouse model of atherosclerosis is associated with downregulation of adhesion molecules.
    Authors: Tikellis C, Jandeleit-Dahm KA, Sheehy K, Murphy A, Chin-Dusting J, Kling D, Sebokova E, Cooper ME, Mizrahi J, Woollard KJ
    Atherosclerosis, 2008-02-21;199(1):55-64.
    Species: Mouse
    Sample Types: Plasma
  16. Rapid conditional targeted ablation of cells expressing human CD59 in transgenic mice by intermedilysin.
    Authors: Hu W, Ferris SP, Tweten RK, Wu G, Radaeva S, Gao B, Bronson RT, Halperin JA, Qin X
    Nat. Med., 2007-12-23;14(1):98-103.
    Species: Mouse
    Sample Types: Plasma
  17. Elimination of platelet factor 4 (PF4) from platelets reduces atherosclerosis in C57Bl/6 and apoE-/- mice.
    Authors: Sachais BS, Turrentine T, Dawicki McKenna JM, Rux AH, Rader D, Kowalska MA
    Thromb. Haemost., 2007-11-01;98(5):1108-13.
    Species: Mouse
    Sample Types: Platelet Poor Plasma
  18. Comparison of adenoviruses from species B, C, e, and f after intravenous delivery.
    Authors: Stone D, Liu Y, Li ZY, Tuve S, Strauss R, Lieber A
    Mol. Ther., 2007-09-25;15(12):2146-53.
    Species: Mouse
    Sample Types: Serum
  19. Endogenous hydrogen sulfide regulates leukocyte trafficking in cecal ligation and puncture-induced sepsis.
    Authors: Zhang H, Zhi L, Moochhala SM, Moore PK, Bhatia M
    J. Leukoc. Biol., 2007-06-28;82(4):894-905.
    Species: Mouse
    Sample Types: Tissue Homogenates
  20. Cell-based angiopoietin-1 gene therapy for acute lung injury.
    Authors: McCarter SD, Mei SH, Lai PF, Zhang QW, Parker CH, Suen RS, Hood RD, Zhao YD, Deng Y, Han RN, Dumont DJ, Stewart DJ
    Am. J. Respir. Crit. Care Med., 2007-02-22;175(10):1014-26.
    Species: Mouse
    Sample Types: Plasma
  21. A fibrinogen deficiency accelerates the initiation of LDL cholesterol-driven atherosclerosis via thrombin generation and platelet activation in genetically predisposed mice.
    Authors: Iwaki T, Sandoval-Cooper MJ, Brechmann M, Ploplis VA, Castellino FJ
    Blood, 2006-01-24;107(10):3883-91.
    Species: Mouse
    Sample Types: Plasma

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Mouse sP-Selectin/CD62P Quantikine ELISA Kit
By Anonymous on 10/12/2021
Sample Tested: Citrate Plasma

We diluted the plasma 1:25


Mouse sP-Selectin/CD62P Quantikine ELISA Kit
By Anonymous on 09/14/2019
Sample Tested: Serum and Plasma