Mouse ST2/IL-33 R Quantikine ELISA Kit

  (3 citations)     
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Assay Procedure
Citations (3)
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  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
  • Sensitivity
    0.059 ng/mL
  • Assay Range
    0.2 - 10 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
  • Specificity
    Natural and recombinant mouse ST2
  • Cross-reactivity
    Cross-reactivity observed with 1 or more available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    Interference observed with 1 or more available related molecules.
Product Summary
The Quantikine Mouse ST2/IL-33 R Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse ST2 in cell culture supernates, serum, and plasma. It contains Sf 21-expressed recombinant mouse ST2 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant factor accurately. Results obtained using natural mouse ST2 showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse ST2.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 0.465 1.34 3.81 0.512 1.43 3.94
Standard Deviation 0.019 0.042 0.106 0.047 0.082 0.138
CV% 4.1 3.1 2.8 9.2 5.7 3.5

Recovery

The recovery of mouse ST2 spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=4) 98 81-111
EDTA Plasma (n=4) 104 92-118
Heparin Plasma (n=4) 94 86-103
Serum (n=4) 103 89-113
Linearity
To assess the linearity of the assay, samples containing high concentrations of mouse ST2 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
 ST2/IL-1 R4 [HRP]
Product Datasheets

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Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: ST2/IL-33 R
ST2, also known as IL-1 R4, is a component of the receptor for the proinflammatory cytokine Interleukin-33. The IL-33 receptor additionally includes IL-1 RAcp which also associates with IL-1 RI and IL-1 R rp2/IL-1 R6. A soluble form of ST2 functions as a decoy receptor. IL-33 promotes Th2-biased immune responses, resulting in eosinophilia, mast cell activation, and allergic inflammation. In addition, IL-33 enhances inflammation-associated hypernociception and protects from atherosclerosis and cardiac myocyte hypertrophy. Soluble ST2 is elevated in the serum under inflammatory conditions including allergic asthma, sepsis, trauma, dengue fever, pulmonary disease, and lupus. It is also associated aortic stenosis, congestive cardiomyopathy, and risk of cardiovascular heart failure and death.
  • Long Name:
    Interleukin 33 Receptor
  • Entrez Gene IDs:
    9173 (Human); 17082 (Mouse)
  • Alternate Names:
    DER4; Fit-1; IL-1 R4; IL1R4; IL-1R4; IL-1RL1; IL-33R; Ly84; ST2; ST2L; ST2V; suppression of tumorigenicity 2; T1
Related Research Areas
Assay Procedure
Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 50 µL Assay Diluent
  4.   Add 50 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  7.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.

  8. 100 µL Conjugate
  9.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
  10.   Aspirate and wash 5 times.

  11. 100 µL Substrate Solution
  12.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.

  13. 100 µL Stop Solution
  14.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Species
Sample Type
  1. Soluble IL-33 receptor sST2 inhibits colorectal cancer malignant growth by modifying the tumour microenvironment
    Nat Commun, 2016;7(0):13589.
    Species: Mouse
    Sample Type: Cell Culture Supernates
  2. Fibrates ameliorate the course of bacterial sepsis by promoting neutrophil recruitment via CXCR2.
    Authors: Tancevski I, Nairz M, Duwensee K, Auer K, Schroll A, Heim C, Feistritzer C, Hoefer J, Gerner R, Moschen A, Heller I, Pallweber P, Li X, Theurl M, Demetz E, Wolf A, Wolf D, Eller P, Ritsch A, Weiss G
    EMBO Mol Med, 2014;6(6):810-20.
    Species: Mouse
    Sample Type: Cell Culture Supernates
  3. Vasoactive intestinal peptide downregulates proinflammatory TLRs while upregulating anti-inflammatory TLRs in the infected cornea.
    Authors: Jiang X, McClellan SA, Barrett RP, Zhang Y, Hazlett LD
    J. Immunol., 2012;189(1):269-78.
    Species: Mouse
    Sample Type: Tissue Homogenates

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