Mouse Syndecan-3 Alexa Fluor® 405-conjugated Antibody

Catalog #: AF2734V Datasheet / COA / SDS

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AF2734V-100UG

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Mouse Syndecan-3 Alexa Fluor® 405-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Syndecan-3 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human Syndecan-3 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Syndecan-3
Ala45-Glu384
Accession # Q64519

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 405 (Excitation= 405 nm, Emission= 421 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Syndecan-3

Mouse Syndecan-3 (also known as N-Syndecan) is a variably glycosylated type I transmembrane (TM) protein that belongs to the Syndecan family. It is synthesized as a 442 amino acid (aa) precursor with a 45 aa signal sequence, a 339 aa extracellular domain (ECD), a 25 aa TM segment and a 33 aa cytoplasmic region. It presumably exists as a dimer. Heparan sulfate modification of the ECD yields a native molecular weight that exceeds 200 kDa. Soluble forms generated by proteolytic processing are suggested to exist. Syndecan-3 is a musculoskeletal and neuronal molecule which mediates neurite outgrowth and myoblast differentiation. Mouse Syndecan-3 ECD shares 95% and 81% aa sequence identity with rat and human Syndecan-3 ECD, respectively.

Entrez Gene IDs

9672 (Human); 20970 (Mouse)

Alternate Names

KIAA0468; N-Syndecan; SDC3; SDCN; SYND3syndecan proteoglycan 3; syndecan 3 (N-syndecan); syndecan 3; syndecan neural type; Syndecan3; Syndecan-3