Mouse Syndecan‑3 Antibody

Syndecan‑3 in Mouse Kidney.

Syndecan‑3 was detected in immersion fixed paraffin-embedded sections of mouse kidney using Goat Anti-Mouse Syndecan‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2734) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane of epithelial cells in convoluted tubules. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of Syndecan-3 by Western Blot

Loss of SCD3 in MSCs causes increased ATK phosphorylation and a reduction in ERK phosphorylation. MSCs were isolated from wild type (n = 6) and Sdc3−/− (n = 6) mice, cultured in full growth medium, and lysed ready for western blotting. (a) Representative images of blots used for quantification. SDC3 knockout was confirmed for Sdc3−/− MSCs in each sample. (b) Total phosphorylated tyrosine normalised to GAPDH. (c) Phosphorylated AKTSer473 was normalised to total AKT. (d) Phosphorylated ERKThr202/Tyr204 was normalised to total ERK. Quantification was performed using ImageJ. Data displayed as mean ± standard deviation; *p < 0.05; **p < 0.01. Full-length blots are presented in Supplementary Figure 1. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33235244), licensed under a CC-BY license. Not internally tested by R&D Systems.