Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all Syndecan-3 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Syndecan-3
Ala45-Glu384
Accession # Q64519

Specificity

Detects mouse Syndecan-3 in Western blots. In Western blots, less than 2% cross-reactivity with recombinant mouse (rm) Syndecan-1, recombinant human (rh) Syndecan-2, rhSyndecan-3, and rmSyndecan-4 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Mouse Syndecan‑3 Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse Syndecan‑3

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Syndecan-3

Mouse Syndecan-3 (also known as N-Syndecan) is a variably glycosylated type I transmembrane (TM) protein that belongs to the Syndecan family. It is synthesized as a 442 amino acid (aa) precursor with a 45 aa signal sequence, a 339 aa extracellular domain (ECD), a 25 aa TM segment and a 33 aa cytoplasmic region. It presumably exists as a dimer. Heparan sulfate modification of the ECD yields a native molecular weight that exceeds 200 kDa. Soluble forms generated by proteolytic processing are suggested to exist. Syndecan-3 is a musculoskeletal and neuronal molecule which mediates neurite outgrowth and myoblast differentiation. Mouse Syndecan-3 ECD shares 95% and 81% aa sequence identity with rat and human Syndecan-3 ECD, respectively.

Alternate Names

N-Syndecan, SDC3, Syndecan3

Entrez Gene IDs

9672 (Human); 20970 (Mouse)

Gene Symbol

SDC3

UniProt

Q64519

Additional Syndecan-3 Products

Product Documents for Mouse Syndecan‑3 Biotinylated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Syndecan‑3 Biotinylated Antibody

For research use only

Related Research Areas

GDNF Family
Nutrition and Appetite
Obesity

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