Mouse TACI/TNFRSF13B Biotinylated Antibody

(1 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse TACI/TNFRSF13B in ELISAs and Western blots. In sandwich ELISAs, less than 0.1% cross-reactivity with recombinant human TACI and recombinant mouse BCMA is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse TACI/TNFRSF13B
    Phe5-Thr129
    Accession # Q9ET35
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
  • Label
    Biotin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse TACI/TNFRSF13B Fc Chimera (Catalog # 1041-TC)
  • Immunohistochemistry
    5-15 µg/mL
    See below
    • Mouse TACI/TNFRSF13B Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Mouse TACI/TNFRSF13B Antibody (Catalog # MAB1041)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Mouse TACI/TNFRSF13B Biotinylated Antibody (Catalog # BAF1041)
  • ELISA Standard
     
    Recombinant Mouse TACI/TNFRSF13B Fc Chimera Protein, CF (Catalog # 1041-TC)
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
TACI/TNFRSF13B in Mouse Thymus. TACI/TNFRSF13B was detected in perfusion fixed frozen sections of mouse thymus using Goat Anti-Mouse TACI/TNFRSF13B Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1041) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TACI/TNFRSF13B

TACI, transmembrane activator and CAML-interactor, is a member of the TNF receptor superfamily and has been designated TNFRSF13B. TACI is a type III membrane protein with an extracellular N-terminus in the absence of a cleaved signal sequence. The extracellular region of TACI contains two cysteine-rich domains. Within the TNFRSF, it shares the highest homology with B cell maturation factor (BCMA). TACI and BCMA have both been shown to bind APRIL and BAFF, members of the TNF ligand superfamily. TACI is expressed on the cell surface of B cells and activated, but not resting, T cells. Analogous to BCMA, data suggests that TACI may play an important role in B cell development, function and regulation. Mouse TACI is a 249 amino acid (aa) protein consisting of a 129 aa extracellular domain, a 20 aa transmembrane domain, and a 100 aa intracellular domain. Mouse and human TACI share 54% aa identity.

  • References:
    1. Xia, X.Z. et al. (2000) J. Exp. Med. 192:137.
    2. von Bulow, G.U. et al. (1997) Science 278:138.
    3. Gross, J.A. et al. (2000) Nature 404:995.
    4. Marsters, S.A. et al. (2000) Curr. Biol. 10:785.
    5. Yan, M. et al. (2000) Nature Immunol. 1:37.
    6. Wu, Y. et al. (2000) J. Biol. Chem. 275:35478.
  • Long Name:
    Transmembrane Activator and CAML Interactor/Tumor Necrosis Factor Receptor Superfamily Member 13B
  • Entrez Gene IDs:
    23495 (Human); 57916 (Mouse)
  • Alternate Names:
    CD267 antigen; CD267; CVID; CVID2; FLJ39942; MGC133214; TACI; TACIMGC39952; TNFRSF13B; TNFRSF14B; Transmembrane activator and CAML interactor; tumor necrosis factor receptor 13B; tumor necrosis factor receptor superfamily member 13B; tumor necrosis factor receptor superfamily, member 13B
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. The transmembrane activator TACI triggers immunoglobulin class switching by activating B cells through the adaptor MyD88.
    Authors: He B, Santamaria R, Xu W, Cols M, Chen K, Puga I, Shan M, Xiong H, Bussel JB, Chiu A, Puel A, Reichenbach J, Marodi L, Doffinger R, Vasconcelos J, Issekutz A, Krause J, Davies G, Li X, Grimbacher B, Plebani A, Meffre E, Picard C, Cunningham-Rundles C, Casanova JL, Cerutti A
    Nat. Immunol., 2010;11(9):836-45.
    Species: Human
    Sample Type: Cell Lysates
    Application: IP
Immunohistochemistry Reagents
Description Application Cat# Citations Images  

Antigen Retrieval Reagent-Universal

CTS015 10
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Antigen Retrieval Reagent-Basic

CTS013 4
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Mounting Medium

CTS011 4
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Antigen Retrieval Reagent Sampler (50 mL each)

CTS016
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Antigen Retrieval Reagent-Acidic

CTS014
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NorthernLights Guard Mounting Media

NL996
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VisUCyte Antigen Retrieval Reagent-Universal

VCTS023
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VisUCyte Antigen Retrieval Reagent-Basic

VCTS021
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VisUCyte Antigen Retrieval Reagent-Acidic

VCTS022
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Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Biotinylated Control

Ctrl BAF108 19
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Staining Reagents
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Streptavidin-Phycoerythrin

Flow F0040 7
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Streptavidin-Alkaline Phosphatase

ELISA(Det) AR001 2
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NorthernLights Streptavidin NL557

Flow, IHC, ICC NL999 4
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NorthernLights Streptavidin NL637

Flow, IHC, ICC NL998 2
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NorthernLights Streptavidin NL493

Flow, IHC, ICC NL997 1
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Streptavidin-Allophycocyanin

Flow F0050 1
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Avidin-Fluorescein

Flow F0030 1
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Supplemental Cell Selection Products
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MagCellect Streptavidin Ferrofluid

MAG999B 4
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MagCellect Streptavidin Ferrofluid

MAG999 4
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Supplemental ELISA Products
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Streptavidin-HRP

ELISA DY998 44
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EvenCoat Streptavidin Coated Plates

ELISA CP004
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EvenCoat Streptavidin Coated Plates, 5 Pack

ELISA CP003 3
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