Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse

Applications

Validated:

Western Blot, Blockade of Receptor-ligand Interaction, Flow Cytometry, CyTOF-ready

Cited:

Western Blot, Neutralization, Flow Cytometry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all TACI/TNFRSF13B Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse TACI/TNFRSF13B
Phe5-Thr129
Accession # Q9ET35

Specificity

Detects mouse TACI/TNFRSF13B in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human TACI is observed and less than 1% cross-reactivity with recombinant mouse BCMA/TNFRSF17 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Applications for Mouse TACI/TNFRSF13B Antibody

Application
Recommended Usage

Blockade of Receptor-ligand Interaction

In a functional ELISA, 0.25-1 µg/mL of this antibody will block 50% of the binding of 100 ng/mL of Recombinant Mouse BAFF/BLyS/TNFSF13B (Catalog # 2106-BF) to immobilized Recombinant Mouse TACI/TNFRSF13B Fc Chimera (Catalog # 1041-TC) coated at 1 µg/mL (100 µL/well). At 15 μg/mL, this antibody will block >90% of the binding.

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: Mouse splenocytes

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse TACI/TNFRSF13B Fc Chimera (Catalog # 1041-TC)

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TACI/TNFRSF13B

TACI, transmembrane activator and CAML-interactor, is a member of the TNF receptor superfamily and has been designated TNFRSF13B. TACI is a type III membrane protein with an extracellular N-terminus in the absence of a cleaved signal sequence. The extracellular region of TACI contains two cysteine-rich domains. Within the TNFRSF, it shares the highest homology with B cell maturation factor (BCMA). TACI and BCMA have both been shown to bind APRIL and BAFF, members of the TNF ligand superfamily. TACI is expressed on the cell surface of B cells and activated, but not resting, T cells. Analogous to BCMA, data suggests that TACI may play an important role in B cell development, function and regulation. Mouse TACI is a 249 amino acid (aa) protein consisting of a 129 aa extracellular domain, a 20 aa transmembrane domain, and a 100 aa intracellular domain. Mouse and human TACI share 54% aa identity.

References

  1. Xia, X.Z. et al. (2000) J. Exp. Med. 192:137.
  2. von Bulow, G.U. et al. (1997) Science 278:138.
  3. Gross, J.A. et al. (2000) Nature 404:995.
  4. Marsters, S.A. et al. (2000) Curr. Biol. 10:785.
  5. Yan, M. et al. (2000) Nature Immunol. 1:37.
  6. Wu, Y. et al. (2000) J. Biol. Chem. 275:35478.

Long Name

Transmembrane Activator and CAML Interactor/Tumor Necrosis Factor Receptor Superfamily Member 13B

Alternate Names

CD267, CVID, TNFRSF13B

Entrez Gene IDs

23495 (Human); 57916 (Mouse); 102126451 (Cynomolgus Monkey)

Gene Symbol

TNFRSF13B

UniProt

Q9ET35

Additional TACI/TNFRSF13B Products

Product Documents for Mouse TACI/TNFRSF13B Antibody

Certificate of Analysis

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Product Specific Notices for Mouse TACI/TNFRSF13B Antibody

For research use only

Citations for Mouse TACI/TNFRSF13B Antibody

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