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Mouse TER-119 Erythroid Antigen MAb (Clone TER-119)

Catalog #: MLDP8 Datasheet / COA / SDS
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Mouse TER-119 Erythroid Antigen MAb (Clone TER-119) Summary

Species Reactivity
Mouse
Specificity
This antibody has been shown to react with cells of the erythroid lineage in embryonic yolk sac, fetal liver, adult bone marrow, adult peripheral blood and adult lymphoid organs.1 TER-119 antigen is a molecule associated with cell-surface glycophorin A.2 The antibody has been shown to react with erythroid cells at differentiation stages from early proerythroblast to mature erythrocyte.2
Source
Monoclonal Rat IgG2b Clone # TER-119
Label
Unconjugated

Applications

Recommended Concentration
Sample
Cell depletion
This antibody has been used in combination with antibodies to other lineage markers to deplete the cells of major hematopoietic lineages. Each vial contains sufficient antibody to deplete TER-119 erythroid antigen+ cells from 1 x 109 bone marrow cells.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Cell Depletion View Larger

Figure 1:Lineage positive bone marrow cells from C57/Bl6 mice were depleted using monoclonal anti-mouse CD3, CD4, CD5, CD8 alpha, CD11b/MAC-1 alpha, B220, Gr-1 (Ly-6G) and TER-119 erythroid antigen antibodies and stained with goat anti-rat IgG PE-conjugated. Flow cytometric analysis was performed A) Before (dotted histogram) and after (solid histogram) the depletion antibody cocktail was added; B) Before (dotted histogram) and after (solid histogram) magnetic depletion using Dynabeads; and C) Expression of CD117 (SCF R/c-kit) (R&D Systems Catalog # MAB1356; dotted histogram) in bone marrow-progenitor cells after enrichment using the mouse lineage panel and bead depletion.

Cell Depletion View Larger

Cell Depletion View Larger

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: TER-119

The TER-119 Antigen has been used as a marker for erythroid cells from the early proerythroblast to mature erythrocyte stages of development. The 52-kDa TER-119 Antigen is associated with Glycophorin A on erythrocytes.

References
  1. Current Protocols in Immunology, Isolation of Murine Macrophages, copyright 1994, Coligan, J.E. et al. eds., John Wiley & Sons, Inc., Volume 3, Supplement 11, 14.1.4.
Entrez Gene IDs
104231 (Mouse)
Alternate Names
Ly76; Ly-76; TER119; TER-119

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Product Specific Notices

Dynabeads® is a registered trademark of Dynal Biotech, Lake Success, NY.

FAQs

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