Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse, Transgenic Mouse

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry, Flow Cytomery

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Polyclonal Goat IgG
View all TGF-beta RII Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse TGF‑ beta  RII and S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse TGF‑ beta  RII
Ile24-Asp184
Accession # Q62312

Specificity

Detects mouse TGF-beta  RII in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) TGF-beta  RII is observed and less than 1% cross-reactivity with recombinant mouse TGF-beta  RI and rhTGF-beta  RIII is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse TGF‑ beta  RII PE‑conjugated Antibody

Detection of TGF‑ beta RII antibody in Mouse Splenocytes antibody by Flow Cytometry.

Detection of TGF‑ beta RII in Mouse Splenocytes by Flow Cytometry.

Mouse splenocytes were stained with Goat Anti-Mouse TGF-beta RII PE-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB532P, filled histogram) or isotype control antibody (Catalog # IC108P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of TGF-beta RII PE by Flow Cytometry

Detection of TGF-beta RII PE by Flow Cytometry

miR-21 silencing induces an antiinflammatory phenotype and upregulates TGF-beta –related pathway in macrophages. Macrophages transfected with an miR-21 antagomir (antagomir-21) or scramble control followed by RT-qPCR and flow cytometry. (A) miR-21-5p fold change in BMDMs after 48-hour transfection with antagomir-21 (n = 8). (B) Spry2 mRNA fold change in BMDMs after 48-hour transfection with antagomir-21 (n = 8). (C) Tgfb1 fold change in BMDMs after transfection with antagomir-21, n = 8 per group pooled from 2 independent experiments. (D) Spearman’s correlation between miR-21-5p expression and Tgfb1 mRNA expression (n = 11). (E) RT-qPCR of Tgfbr2, Tgfbr1, Tgfbr3, and Smad7 in BMDMs after 48-hour transfection with antagomir-21 (n = 8). (F) RT-qPCR of proinflammatory genes Tnfa and Il6 in BMDMs transfected with antagomir-21 (n = 8). (G) RT-qPCR of antiinflammatory genes Mrc1 and Il10, n = 8 per group, pooled from 2 independent experiments. (H) Flow cytometry analysis of TGF-beta R2 expression in BMDMs after silencing miR-21-5p, n = 4 per group. The bar graphs represent the percentage of F4/80+TGF-beta R2+ cells. Data are presented as mean ± SEM. *P < 0.05, **P < 0.01 by unpaired, 2-tailed Student’s t test (A–C and E–G). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37071481), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of TGF-beta RII PE by Flow Cytometry

Detection of TGF-beta RII PE by Flow Cytometry

miR-21 silencing induces an antiinflammatory phenotype and upregulates TGF-beta –related pathway in macrophages. Macrophages transfected with an miR-21 antagomir (antagomir-21) or scramble control followed by RT-qPCR and flow cytometry. (A) miR-21-5p fold change in BMDMs after 48-hour transfection with antagomir-21 (n = 8). (B) Spry2 mRNA fold change in BMDMs after 48-hour transfection with antagomir-21 (n = 8). (C) Tgfb1 fold change in BMDMs after transfection with antagomir-21, n = 8 per group pooled from 2 independent experiments. (D) Spearman’s correlation between miR-21-5p expression and Tgfb1 mRNA expression (n = 11). (E) RT-qPCR of Tgfbr2, Tgfbr1, Tgfbr3, and Smad7 in BMDMs after 48-hour transfection with antagomir-21 (n = 8). (F) RT-qPCR of proinflammatory genes Tnfa and Il6 in BMDMs transfected with antagomir-21 (n = 8). (G) RT-qPCR of antiinflammatory genes Mrc1 and Il10, n = 8 per group, pooled from 2 independent experiments. (H) Flow cytometry analysis of TGF-beta R2 expression in BMDMs after silencing miR-21-5p, n = 4 per group. The bar graphs represent the percentage of F4/80+TGF-beta R2+ cells. Data are presented as mean ± SEM. *P < 0.05, **P < 0.01 by unpaired, 2-tailed Student’s t test (A–C and E–G). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37071481), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse TGF‑ beta  RII PE‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

0.25-1 µg/106 cells
Sample: Mouse splenocytes

Reviewed Applications

Read 2 reviews rated 4.5 using FAB532P in the following applications:

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: TGF-beta RII

Most cell types express three sizes of receptors for TGF-beta. These are designated Type I (53 kDa), Type II (70-85 kDa), and Type III (250-350 kDa). The Type III receptor, a proteoglycan that exists in membrane-bound and soluble forms, binds TGF-beta 1, TGF-beta 2, and TGF-beta 3 but does not appear to be involved in signal transduction. The Type II receptor is a membrane-bound serine/threonine kinase that binds TGF-beta 1 and TGF-beta 3 with high affinity and TGF-beta 2 with a much lower affinity. The Type I receptor is also a membrane-bound serine/threonine kinase that apparently requires the presence of the Type II receptor to bind TGF-beta. Current evidence suggests that signal transduction requires the cytoplasmic domains of both the Type I and Type II receptors.

The recombinant soluble TGF-beta Type II receptor is capable of binding TGF-beta 1, TGF-beta 3, and TGF-beta 5 with sufficient affinity to act as an inhibitor of these isoforms at high concentrations. The soluble receptor also binds TGF-beta 2, but with an affinity at least two orders of magnitude lower. Binding of TGF-beta 1, TGF-beta 3, and TGF-beta 5 to the soluble TGF-beta Type II receptor can also be demonstrated by using the soluble receptor as a capture agent on ELISA plates and this observation has been used as the basis for the development of immunoassays for these isoforms of TGF-beta.

References

  1. Miyazono, K. et al. (1994) Adv. in Immunol. 55:181.

Long Name

Transforming Growth Factor beta Receptor II

Alternate Names

TGF-bRII, TGFbetaRII, TGFBR2

Entrez Gene IDs

7048 (Human); 21813 (Mouse)

Gene Symbol

TGFBR2

UniProt

Q62312

Additional TGF-beta RII Products

Product Documents for Mouse TGF‑ beta  RII PE‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse TGF‑ beta  RII PE‑conjugated Antibody

For research use only

Citations for Mouse TGF‑ beta  RII PE‑conjugated Antibody

Customer Reviews for Mouse TGF‑ beta  RII PE‑conjugated Antibody (2)

4.5 out of 5
2 Customer Ratings
5 Stars
50%
4 Stars
50%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Mouse TGF‑ beta  RII PE‑conjugated Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 2 of 2 reviews Showing All
Filter By:
Clear Filters X
  • Mouse TGF-beta  RII PE-conjugated Antibody
    Name: Anonymous
    Application: Cell Culture
    Sample Tested: Bone marrow cells
    Species: Mouse
    Verified Customer | Posted 06/17/2022
    Mouse TGF‑ beta  RII PE‑conjugated Antibody FAB532P
  • Mouse TGF-beta  RII PE-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Peritoneal resident macrophages
    Species: Mouse
    Verified Customer | Posted 05/12/2022
    Followed the reference protocol, should incubate with TGFBRII-PE antibody in 37℃ 30mins, then stained other antibodies in 4℃.
    Mouse TGF‑ beta  RII PE‑conjugated Antibody FAB532P

There are no reviews that match your criteria.

Showing  1 - 2 of 2 reviews Showing All

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies