Mouse TGN38 Alexa Fluor™ Plus 488‑conjugated Antibody

R&D Systems | Catalog # FAB7944AFP488

R&D Systems

Key Product Details

Species Reactivity

Mouse

Applications

Western Blot, Immunocytochemistry

Label

Alexa Fluor Plus 488 (Excitation = 493 nm, Emission = 518 nm)

Antibody Source

Monoclonal Rat IgG2B Clone # 831629
View all TGN38 Primary Antibodies »

Product Specifications

Specificity

Detects mouse TGN38 in ELISAs.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Applications for Mouse TGN38 Alexa Fluor™ Plus 488‑conjugated Antibody

Application
Recommended Usage

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: TGN38

TGN38A (also Tgoln1, Trans-Golgi network integral membrane protein 1) is an integral membrane protein associated with intracellular protein trafficking. Although its predicted MW is 38 kDa, due to a high proline content and extensive polysialylation, it runs anomalously at 80-90 kDa in SDS-PAGE. Tgoln1 is ubiquitously expressed and is generally associated with the trans-Golgi complex, a structure adjacent to the trans-component of the Golgi Apparatus. In this complex, both secretory and membrane proteins are sorted and forwarded to various compartments such as lysosomes, endosomes, the cell membrane and secretory granules. TGN38A apparently cycles between the trans-Golgi network and the cell membrane, returning to the trans-Golgi via the endosomal system. Part of its mobility is mediated by an interaction between TGN38A, neurabin and actin. Integrin beta 1 is one molecule suggested to be transported by TGN38A.  Mature mouse TGN38A is a 336 amino acid (aa) type I transmembrane glycoprotein.  It contains a 281 aa extracellular region (aa 18-298) plus a 34 aa cytoplasmic domain.  The extracellular region possesses six sequential octapeptide repeats (aa 131-178) plus two utilized phosphorylation sites (Ser230 and Ser231).  The cytoplasmic domain contains an endocytosis signal (Ser344-Leu349) that requires a free hydroxyl on Ser344 for proper routing. The mouse genome has two Tgn38 genes, Tgn38A and Tgn38B/Tgoln2.  They differ in two ways. First there is a two aa insertion after Pro47, and an eight aa insertion after Thr154 in Tgn38B and, second, Tgn38B expression is restricted, occurring in mouse strain ICR, while Tgn38A is widespread and found in strains ICR, BALB/c and C57BL/6.  Over aa 320-349, mouse and rat Tgoln1 are identical in aa sequence.  There is no strict human structural counterpart to mouse Tgoln1/Tgn38A.  Human does, however, possess a similar functioning molecule termed TGN46/48/51, and over the short aa stretch 320-349, mouse and human TGN molecules share 96% aa sequence identity.

Long Name

Trans-golgi Network Protein

Alternate Names

TGN46, TGN51, Tgoln1, Tgoln2, Ttgn1

Entrez Gene IDs

10618 (Human); 22134 (Mouse); 192152 (Rat)

Gene Symbol

TGOLN2

UniProt

Q62313

Additional TGN38 Products

Product Documents for Mouse TGN38 Alexa Fluor™ Plus 488‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse TGN38 Alexa Fluor™ Plus 488‑conjugated Antibody


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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Protocols

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