Mouse TGN38 Antibody

Catalog # Availability Size / Price Qty
MAB7944
MAB7944-SP

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Detection of Mouse TGN38 by Western Blot.
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Mouse TGN38 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse TGN38 in ELISAs.
Source
Monoclonal Rat IgG2B Clone # 831629
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse TGN38
His320-Leu349
Accession # Q62313
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Mouse TGN38 antibody by Western Blot. View Larger

Detection of Mouse TGN38 by Western Blot. Western blot shows lysates of Neuro-2A mouse neuroblastoma cell line and C2C12 mouse myoblast cell line. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse TGN38 Monoclonal Antibody (Catalog # MAB7944) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for TGN38 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry TGN38 antibody in NIH-3T3 Mouse Cell Line by Immunocytochemistry (ICC). View Larger

TGN38 in NIH‑3T3 Mouse Cell Line. TGN38 was detected in immersion fixed NIH-3T3 mouse embryonic fibroblast cell line using Rat Anti-Mouse TGN38 Monoclonal Antibody (Catalog # MAB7944) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to vesicles in the cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TGN38

TGN38A (also Tgoln1, Trans-Golgi network integral membrane protein 1) is an integral membrane protein associated with intracellular protein trafficking. Although its predicted MW is 38 kDa, due to a high proline content and extensive polysialylation, it runs anomalously at 80-90 kDa in SDS-PAGE. Tgoln1 is ubiquitously expressed and is generally associated with the trans-Golgi complex, a structure adjacent to the trans-component of the Golgi Apparatus. In this complex, both secretory and membrane proteins are sorted and forwarded to various compartments such as lysosomes, endosomes, the cell membrane and secretory granules. TGN38A apparently cycles between the trans-Golgi network and the cell membrane, returning to the trans-Golgi via the endosomal system. Part of its mobility is mediated by an interaction between TGN38A, neurabin and actin. Integrin beta 1 is one molecule suggested to be transported by TGN38A.  Mature mouse TGN38A is a 336 amino acid (aa) type I transmembrane glycoprotein.  It contains a 281 aa extracellular region (aa 18-298) plus a 34 aa cytoplasmic domain.  The extracellular region possesses six sequential octapeptide repeats (aa 131-178) plus two utilized phosphorylation sites (Ser230 and Ser231).  The cytoplasmic domain contains an endocytosis signal (Ser344-Leu349) that requires a free hydroxyl on Ser344 for proper routing. The mouse genome has two Tgn38 genes, Tgn38A and Tgn38B/Tgoln2.  They differ in two ways. First there is a two aa insertion after Pro47, and an eight aa insertion after Thr154 in Tgn38B and, second, Tgn38B expression is restricted, occurring in mouse strain ICR, while Tgn38A is widespread and found in strains ICR, BALB/c and C57BL/6.  Over aa 320-349, mouse and rat Tgoln1 are identical in aa sequence.  There is no strict human structural counterpart to mouse Tgoln1/Tgn38A.  Human does, however, possess a similar functioning molecule termed TGN46/48/51, and over the short aa stretch 320-349, mouse and human TGN molecules share 96% aa sequence identity.

Long Name
Trans-golgi Network Protein
Entrez Gene IDs
10618 (Human); 22134 (Mouse); 192152 (Rat)
Alternate Names
TGN38; TGN46; TGN46TGN38 homolog; TGN48MGC14722; TGN51; TGN5148, 51kD isoforms); Tgoln1; Tgoln2; trans-golgi network protein 2; Trans-Golgi network protein TGN51; Ttgn1

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