Mouse TGN38 Alexa Fluor® 750-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: TGN38

TGN38A (also Tgoln1, Trans-Golgi network integral membrane protein 1) is an integral membrane protein associated with intracellular protein trafficking. Although its predicted MW is 38 kDa, due to a high proline content and extensive polysialylation, it runs anomalously at 80-90 kDa in SDS-PAGE. Tgoln1 is ubiquitously expressed and is generally associated with the trans-Golgi complex, a structure adjacent to the trans-component of the Golgi Apparatus. In this complex, both secretory and membrane proteins are sorted and forwarded to various compartments such as lysosomes, endosomes, the cell membrane and secretory granules. TGN38A apparently cycles between the trans-Golgi network and the cell membrane, returning to the trans-Golgi via the endosomal system. Part of its mobility is mediated by an interaction between TGN38A, neurabin and actin. Integrin beta 1 is one molecule suggested to be transported by TGN38A.  Mature mouse TGN38A is a 336 amino acid (aa) type I transmembrane glycoprotein.  It contains a 281 aa extracellular region (aa 18-298) plus a 34 aa cytoplasmic domain.  The extracellular region possesses six sequential octapeptide repeats (aa 131-178) plus two utilized phosphorylation sites (Ser230 and Ser231).  The cytoplasmic domain contains an endocytosis signal (Ser344-Leu349) that requires a free hydroxyl on Ser344 for proper routing. The mouse genome has two Tgn38 genes, Tgn38A and Tgn38B/Tgoln2.  They differ in two ways. First there is a two aa insertion after Pro47, and an eight aa insertion after Thr154 in Tgn38B and, second, Tgn38B expression is restricted, occurring in mouse strain ICR, while Tgn38A is widespread and found in strains ICR, BALB/c and C57BL/6.  Over aa 320-349, mouse and rat Tgoln1 are identical in aa sequence.  There is no strict human structural counterpart to mouse Tgoln1/Tgn38A.  Human does, however, possess a similar functioning molecule termed TGN46/48/51, and over the short aa stretch 320-349, mouse and human TGN molecules share 96% aa sequence identity.

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