Tumor Necrosis Factor Receptor type II (TNF RII), also known as p75 or p80 TNF R, is a prototypic member of the TNF receptor superfamily and has been designated TNFRSF1B. It binds TNF with high affinity and is expressed primarily in cells of the immune system.
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Hamster IgG Clone # TR7532
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Product Specifications
Immunogen
E. coli-derived recombinant mouse TNF RII
Extracellular domain
Accession # P20333
Extracellular domain
Accession # P20333
Specificity
Detects mouse TNF RII in direct ELISAs and Western blots. In direct ELISAs, this antibody does not cross-react with recombinant human (rh) 4-1BB, rm4-1BB, rhAXL, rhCD27, rmCD27, rhCD30, rmCD30, rhCD40, rmCD40, rhDR3, rhDR6, rhEDAR, rmEDAR, rhFas, rmFas, rhGITR, rmGITR, rhHVEM, rhLymphotoxin‑ beta R, rmLymphotoxin‑ beta R, rhNGF R, rhOPG, mOPG, rhRANK, rmRANK, rhTAJ, rhTNF RI, rmTNF RI, rhTNF RII.
Clonality
Monoclonal
Host
Hamster
Isotype
IgG
Applications for Mouse TNF RII/TNFRSF1B Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Mouse TNF RII/TNFRSF1B (Catalog # 426-R2)
under non-reducing conditions only
Sample: Recombinant Mouse TNF RII/TNFRSF1B (Catalog # 426-R2)
under non-reducing conditions only
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: TNF RII/TNFRSF1B
Long Name
Tumor Necrosis Factor Receptor II
Alternate Names
CD120b, TNFRII, TNFRSF1B
Gene Symbol
TNFRSF1B
UniProt
Additional TNF RII/TNFRSF1B Products
Product Documents for Mouse TNF RII/TNFRSF1B Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse TNF RII/TNFRSF1B Antibody
For research use only
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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