Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Hamster IgG Clone # TR7532
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Product Specifications

Immunogen

E. coli-derived recombinant mouse TNF RII
Extracellular domain
Accession # P20333

Specificity

Detects mouse TNF RII in direct ELISAs and Western blots. In direct ELISAs, this antibody does not cross-react with recombinant human (rh) 4-1BB, rm4-1BB, rhAXL, rhCD27, rmCD27, rhCD30, rmCD30, rhCD40, rmCD40, rhDR3, rhDR6, rhEDAR, rmEDAR, rhFas, rmFas, rhGITR, rmGITR, rhHVEM, rhLymphotoxin‑ beta  R, rmLymphotoxin‑ beta  R, rhNGF R, rhOPG, mOPG, rhRANK, rmRANK, rhTAJ, rhTNF RI, rmTNF RI, rhTNF RII.

Clonality

Monoclonal

Host

Hamster

Isotype

IgG

Applications for Mouse TNF RII/TNFRSF1B Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Mouse TNF RII/TNFRSF1B (Catalog # 426-R2)
under non-reducing conditions only

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.


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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TNF RII/TNFRSF1B

Tumor Necrosis Factor Receptor type II (TNF RII), also known as p75 or p80 TNF R, is a prototypic member of the TNF receptor superfamily and has been designated TNFRSF1B. It binds TNF with high affinity and is expressed primarily in cells of the immune system.

Long Name

Tumor Necrosis Factor Receptor II

Alternate Names

CD120b, TNFRII, TNFRSF1B

Entrez Gene IDs

7133 (Human); 21938 (Mouse); 156767 (Rat); 102144224 (Cynomolgus Monkey)

Gene Symbol

TNFRSF1B

UniProt

Additional TNF RII/TNFRSF1B Products

Product Documents for Mouse TNF RII/TNFRSF1B Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse TNF RII/TNFRSF1B Antibody

For research use only

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