Mouse TRAM/TICAM2 Antibody

Catalog #: MAB4348 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4348
MAB4348-SP

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Detection of Mouse TRAM/TICAM2 by Western Blot.

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All TRAM/TICAM2 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse TRAM/TICAM2 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse TRAM/TICAM2 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) TRAM/TICAM2 or rhTRIF/TICAM1 is observed.

Source

Monoclonal Rat IgG_2B Clone # 757706

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant mouse TRAM/TICAM2
Met1-Ala232
Accession # Q8BJQ4

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse TRAM/TICAM2 by Western Blot. Western blot shows lysates of C2C12 mouse myoblast cell line, L-929 mouse fibroblast cell line, and mouse placenta tissue. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse TRAM/TICAM2 Monoclonal Antibody (Catalog # MAB4348) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for TRAM/TICAM2 at approximately 31 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TRAM/TICAM2

The innate and adaptive immune responses depend on systems that link cell surface surveillance receptor signals to cytoplasmic proteins such as kinases, adaptors, and transcription factors. Toll-like receptors (TLR) recognize different pathogen associated molecular patterns (PAMPs), and initiate a signaling cascades mediated by a Toll/interleukin-1 receptor (TIR) domain-containing adaptor proteins such as MyD88, TIRAP/MAL, and TRIF. Mouse TRIF-related adaptor molecule (TRAM), is a 232 amino acid, 26 kDa (predicted), ubiquitously expressed member of the TIR domain-containing adaptor family. TRAM, also known as TIR domain-containing adapter protein 2 (TICAM2) and TIR domain-containing protein (TIRP), contains a central TIR domain that is most similar to that of TRIF. TRAM plays an essential role in the MyD88-independent signaling of TLR4 by binding members of the IRAK family, ultimately leading to the activation of NF kappa B. Mouse TRAM shares 75% and 77% identity to human and rat TRAM, respectively.

Long Name

TRIF-related Adapter Molecule

Entrez Gene IDs

353376 (Human); 225471 (Mouse); 364867 (Rat)

Alternate Names

MGC129876; MGC129877; Putative NF-kappa-B-activating protein 502; TICAM2; TICAM-2TIR domain-containing adapter molecule 2; TIRAP3; TIRP; TIRPcytoplasmic adaptor; toll/interleukin-1 receptor (TIR) domain-containing adapter protein; Toll/interleukin-1 receptor domain-containing protein; toll-like receptor adaptor molecule 2; Toll-like receptor adaptor protein 3; TRAM; TRAMNF-kappa-B-activating protein 502; Trif; TRIF-related adapter molecule; TRIF-related adaptor molecule