Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 757706
View all TRAM/TICAM2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse TRAM/TICAM2
Met1-Ala232
Accession # Q8BJQ4

Specificity

Detects mouse TRAM/TICAM2 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) TRAM/TICAM2 or rhTRIF/TICAM1 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Mouse TRAM/TICAM2 Antibody

Detection of Mouse TRAM/TICAM2 antibody by Western Blot.

Detection of Mouse TRAM/TICAM2 by Western Blot.

Western blot shows lysates of C2C12 mouse myoblast cell line, L-929 mouse fibroblast cell line, and mouse placenta tissue. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse TRAM/TICAM2 Monoclonal Antibody (Catalog # MAB4348) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for TRAM/TICAM2 at approximately 31 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Mouse TRAM/TICAM2 Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: C2C12 mouse myoblast cell line, L‑929 mouse fibroblast cell line, and mouse placenta tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TRAM/TICAM2

The innate and adaptive immune responses depend on systems that link cell surface surveillance receptor signals to cytoplasmic proteins such as kinases, adaptors, and transcription factors. Toll-like receptors (TLR) recognize different pathogen associated molecular patterns (PAMPs), and initiate a signaling cascades mediated by a Toll/interleukin-1 receptor (TIR) domain-containing adaptor proteins such as MyD88, TIRAP/MAL, and TRIF. Mouse TRIF-related adaptor molecule (TRAM), is a 232 amino acid, 26 kDa (predicted), ubiquitously expressed member of the TIR domain-containing adaptor family. TRAM, also known as TIR domain-containing adapter protein 2 (TICAM2) and TIR domain-containing protein (TIRP), contains a central TIR domain that is most similar to that of TRIF. TRAM plays an essential role in the MyD88-independent signaling of TLR4 by binding members of the IRAK family, ultimately leading to the activation of NF kappa B. Mouse TRAM shares 75% and 77% identity to human and rat TRAM, respectively.

Long Name

TRIF-related Adapter Molecule

Alternate Names

TICAM2, TIRAP3, TIRP, Trif

Entrez Gene IDs

353376 (Human); 225471 (Mouse); 364867 (Rat)

Gene Symbol

TICAM2

UniProt

Q8BJQ4

Additional TRAM/TICAM2 Products

Product Documents for Mouse TRAM/TICAM2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse TRAM/TICAM2 Antibody

For research use only

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