Mouse TRANCE/RANK L/TNFSF11 Quantikine ELISA Kit

(28 citations)   
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Serum (25 uL)
  • Sensitivity
    5 pg/mL
  • Assay Range
    31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum)
  • Specificity
    Free natural and recombinant mouse TRANCE
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
  • Interference
    Interference observed with 1 or more available related molecules.
Product Summary
The Quantikine Mouse TRANCE Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse TRANCE in cell culture supernates and serum. It contains NS0-expressed recombinant mouse TRANCE and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate recombinant mouse TRANCE accurately. Results obtained using natural mouse TRANCE showed dose response curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse TRANCE.

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum
Intra-Assay Precision Inter-Assay Precision
Standard Deviation86.818.38.817.857.8


The recovery of mouse TRANCE spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=6) 99 87-116
Serum (n=6) 93 80-104
To assess the linearity of the assay, samples containing and/or spiked with mouse TRANCE were diluted with Calibrator Diluent and assayed.
Mouse TRANCE/RANK L/TNFSF11 Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
TRANCE (TNF-related activation-induced cytokines), also known as RANK Ligand, OPGL, or ODF, is a TNF superfamily ligand produced by osteoblasts, bone marrow stromal cells, and activated T cells. It signals through RANK and is antagonized by the decoy receptor Osteoprotegerin. TRANCE stimulates bone resorption, is required for osteoclast differentiation, and is associated with the development of osteoporosis, vascular calcification, osteopetrosis, and progestin-induced breast cancer. It also mediates body temperature regulation and is essential for the development of a lactating mammary gland.
    • Long Name
      TNF-related Activation-induced Cytokine
    • Entrez Gene IDs
      8600 (Human); 21943 (Mouse);
    • Alternate Names
      CD254 antigen; CD254; ODF; OPGL; OPGLOPTB2; Osteoclast differentiation factor; Osteoprotegerin ligand; RANK L; RANKLreceptor activator of nuclear factor kappa B ligand; Receptor activator of nuclear factor kappa-B ligand; sOdf; TNF-related activation-induced cytokine; TNFSF11; TRANCEODFhRANKL2; tumor necrosis factor (ligand) superfamily, member 11; tumor necrosis factor ligand superfamily member 11;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 50 µL Assay Diluent
    4.   Add 50 µL of Assay Diluent to each well.

    5. 50 µL Standard, Control, or Sample
    6.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
    7.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.

    8. 100 µL Conjugate
    9.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
    10.   Aspirate and wash 5 times.

    11. 100 µL Substrate Solution
    12.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

    13. 100 µL Stop Solution
    14.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    Showing Results 1 - 10 of 28
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    Sample Type
    1. Porphyromonas gingivalis Stimulates Bone Resorption by Enhancing RANKL (Receptor Activator of NF-kappaB Ligand) through Activation of Toll-like Receptor 2 in Osteoblasts.
      Authors: Kassem A, Henning P, Lundberg P, Souza P, Lindholm C, Lerner U
      J Biol Chem, 2015;290(33):20147-58.
      Species: Mouse
      Sample Type: Tissue Homogenates
    2. Effects of neuropeptides and mechanical loading on bone cell resorption in vitro.
      Authors: Yoo Y, Kwag J, Kim K, Kim C
      Int J Mol Sci, 2014;15(4):5874-83.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    3. Resolvin E1 and chemokine-like receptor 1 mediate bone preservation.
      Authors: Gao L, Faibish D, Fredman G, Herrera B, Chiang N, Serhan C, Van Dyke T, Gyurko R
      J Immunol, 2013;190(2):689-94.
      Species: Mouse
      Sample Type: cell culture supernate
    4. The RANKL distal control region is required for the increase in RANKL expression, but not the bone loss, associated with hyperparathyroidism or lactation in adult mice.
      Authors: Onal M, Galli C, Fu Q, Xiong J, Weinstein RS, Manolagas SC, O'Brien CA
      Mol. Endocrinol., 2012;26(2):341-8.
      Species: Mouse
      Sample Type: Plasma
    5. Osteoprotection by semaphorin 3A.
      Authors: Hayashi M, Nakashima T, Taniguchi M, Kodama T, Kumanogoh A, Takayanagi H
      Nature, 2012;485(7396):69-74.
      Species: Mouse
      Sample Type: Serum
    6. Strontium fructose 1,6-diphosphate prevents bone loss in a rat model of postmenopausal osteoporosis via the OPG/RANKL/RANK pathway.
      Authors: Ma B, Zhang Q, Wu D, Wang YL, Hu YY, Cheng YP, Yang ZD, Zheng YY, Ying HJ
      Acta Pharmacol. Sin., 2012;33(4):479-89.
      Species: Rat
      Sample Type: Serum
    7. Soluble rank ligand produced by myeloma cells causes generalised bone loss in multiple myeloma.
      Authors: Buckle, Clive He, De Leenheer, Evy, Lawson, Michelle, Yong, Kwee, Rabin, Neil, Perry, Mark, Vanderkerken, Karen, Croucher, Peter Ia
      PLoS ONE, 2012;7(8):e41127.
      Species: Mouse
      Sample Type: Serum
    8. Receptor activator of nuclear factor kappaB ligand (RANKL) protein expression by B lymphocytes contributes to ovariectomy-induced bone loss.
      Authors: Onal M, Xiong J, Chen X, Thostenson J, Almeida M, Manolagas S
      Oncogene, 2012;287(35):29851-60.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    9. Mechanisms inducing low bone density in duchenne muscular dystrophy in mice and humans.
      Authors: Rufo A, Del Fattore A, Capulli M, Carvello F, De Pasquale L, Ferrari S, Pierroz D, Morandi L, De Simone M, Rucci N, Bertini E, Bianchi ML, De Benedetti F, Teti A
      J. Bone Miner. Res., 2011;26(8):1891-903.
      Species: Mouse
      Sample Type: Serum
    10. C17 prevents inflammatory arthritis and associated joint destruction in mice.
      Authors: Chao C, Joyce-Shaikh B, Grein J
      PLoS ONE, 2011;6(7):e22256.
      Species: Mouse
      Sample Type: Serum
    11. Effects of relaxin and estrogens on bone remodeling markers, receptor activator of NF-kB ligand (RANKL) and osteoprotegerin (OPG), in rat adjuvant-induced arthritis.
      Authors: Ho TY, Santora K, Chen JC, Frankshun AL, Bagnell CA
      Bone, 2011;48(6):1346-53.
      Species: Rat
      Sample Type: Plasma
    12. Increased trabecular bone formation in mice lacking the growth factor midkine.
      Authors: Neunaber C, Catala-Lehnen P, Beil FT
      J. Bone Miner. Res., 2010;25(8):1724-35.
      Species: Mouse
      Sample Type: Serum
    13. Systemic effects of ulna loading in male rats during functional adaptation.
      Authors: Sample SJ, Collins RJ, Wilson AP, Racette MA, Behan M, Markel MD, Kalscheur VL, Hao Z, Muir P
      J. Bone Miner. Res., 2010;25(9):2016-28.
      Species: Rat
      Sample Type: Plasma
    14. Beta-Arrestin2 regulates RANKL and ephrins gene expression in response to bone remodeling in mice.
      Authors: Pierroz DD, Rufo A, Bianchi EN, Glatt V, Capulli M, Rucci N, Cavat F, Rizzoli R, Teti A, Bouxsein ML, Ferrari SL
      J. Bone Miner. Res., 2009;24(5):775-84.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    15. Substance P stimulates bone marrow stromal cell osteogenic activity, osteoclast differentiation, and resorption activity in vitro.
      Authors: Wang L, Zhao R, Shi X, Wei T, Halloran BP, Clark DJ, Jacobs CR, Kingery WS
      Bone, 2009;45(2):309-20.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    16. ADA-deficient SCID is associated with a specific microenvironment and bone phenotype characterized by RANKL/OPG imbalance and osteoblast insufficiency.
      Authors: Sauer AV, Mrak E, Hernandez RJ, Zacchi E, Cavani F, Casiraghi M, Grunebaum E, Roifman CM, Cervi MC, Ambrosi A, Carlucci F, Roncarolo MG, Villa A, Rubinacci A, Aiuti A
      Blood, 2009;114(15):3216-26.
      Species: Mouse
      Sample Type: Serum
    17. Mecp2 deficiency decreases bone formation and reduces bone volume in a rodent model of Rett syndrome.
      Authors: O'Connor RD, Zayzafoon M, Farach-Carson MC, Schanen NC
      Bone, 2009;45(2):346-56.
      Species: Mouse
      Sample Type: Serum
    18. Myeloma-derived Dickkopf-1 disrupts Wnt-regulated osteoprotegerin and RANKL production by osteoblasts: a potential mechanism underlying osteolytic bone lesions in multiple myeloma.
      Authors: Qiang YW, Chen Y, Stephens O, Brown N, Chen B, Epstein J, Barlogie B, Shaughnessy JD
      Blood, 2008;112(1):196-207.
      Species: Human
      Sample Type: Cell Culture Supernates
    19. Osteoprotegerin inhibits vascular calcification without affecting atherosclerosis in ldlr(-/-) mice.
      Authors: Morony S, Tintut Y, Zhang Z, Cattley RC, Van G, Dwyer D, Stolina M, Kostenuik PJ, Demer LL
      Circulation, 2008;117(3):411-20.
      Species: Mouse
      Sample Type: Plasma
    20. The essential role of IFN-gamma in the control of lethal Aggregatibacter actinomycetemcomitans infection in mice.
      Authors: Garlet GP, Cardoso CR, Campanelli AP, Garlet TP, Avila-Campos MJ, Cunha FQ, Silva JS
      Microbes Infect., 2008;10(5):489-96.
      Species: Mouse
      Sample Type: Tissue Homogenates
    21. Comparison of the effects of genistein and zoledronic acid on the bone loss in OPG-deficient mice.
      Authors: Liu J, Xu K, Wen G, Guo H, Li S, Wu X, Dai R, Sheng Z, Liao E
      Bone, 2008;42(5):950-9.
      Species: Mouse
      Sample Type: Serum
    22. Overexpression of bone sialoprotein leads to an uncoupling of bone formation and bone resorption in mice.
      Authors: Valverde P, Zhang J, Fix A, Zhu J, Ma W, Tu Q, Chen J
      J. Bone Miner. Res., 2008;23(11):1775-88.
      Species: Mouse
      Sample Type: Serum
    23. Administration of cyclooxygenase-2 inhibitor reduces joint inflammation but exacerbates osteopenia in IL-1 alpha transgenic mice due to GM-CSF overproduction.
      Authors: Niki Y, Takaishi H, Takito J, Miyamoto T, Kosaki N, Matsumoto H, Toyama Y, Tada N
      J. Immunol., 2007;179(1):639-46.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    24. Attenuation of bone mass and increase of osteoclast formation in decoy receptor 3 transgenic mice.
      Authors: Tang CH, Hsu TL, Lin WW, Lai MZ, Yang RS, Hsieh SL, Fu WM
      J. Biol. Chem., 2007;282(4):2346-54.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    25. Bradykinin potentiates cytokine-induced prostaglandin biosynthesis in osteoblasts by enhanced expression of cyclooxygenase 2, resulting in increased RANKL expression.
      Authors: Brechter AB, Lerner UH
      Arthritis Rheum., 2007;56(3):910-23.
      Species: Mouse
      Sample Type: Whole Tissue
    26. New 19-nor-(20S)-1alpha,25-dihydroxyvitamin D3 analogs strongly stimulate osteoclast formation both in vivo and in vitro.
      Authors: Sato M, Nakamichi Y, Nakamura M, Sato N, Ninomiya T, Muto A, Nakamura H, Ozawa H, Iwasaki Y, Kobayashi E, Shimizu M, DeLuca HF, Takahashi N, Udagawa N
      Bone, 2007;40(2):293-304.
      Species: Mouse
      Sample Type: Serum
    27. B cells and T cells are critical for the preservation of bone homeostasis and attainment of peak bone mass in vivo.
      Authors: Li Y, Toraldo G, Li A, Yang X, Zhang H, Qian WP, Weitzmann MN
      Blood, 2007;109(9):3839-48.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    28. Osteoprotegerin reduces the serum level of receptor activator of NF-kappaB ligand derived from osteoblasts.
      Authors: Nakamichi Y, Udagawa N, Kobayashi Y, Nakamura M, Yamamoto Y, Yamashita T, Mizoguchi T, Sato M, Mogi M, Penninger JM, Takahashi N
      J. Immunol., 2007;178(1):192-200.
      Species: Mouse
      Sample Type: Serum
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