Mouse TRANCE/RANK L/TNFSF11 Quantikine ELISA Kit

R&D Systems | Catalog # MTR00

R&D Systems
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Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 µL), Serum (25 µL)

Sensitivity

5 pg/mL

Assay Range

31.2-2000 pg/mL (Cell Culture Supernates, Serum)
View all TRANCE/TNFSF11/RANK L ELISA Kits »

Product Summary for Mouse TRANCE/RANK L/TNFSF11 Quantikine ELISA Kit

The Quantikine Mouse TRANCE Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse TRANCE in cell culture supernates and serum. It contains NS0-expressed recombinant mouse TRANCE and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate recombinant mouse TRANCE accurately. Results obtained using natural mouse TRANCE showed dose response curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse TRANCE.

Product Specifications

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Species

Mouse

Specificity

Free natural and recombinant mouse TRANCE

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. Cross-species reactivity not tested.

Interference

Interference observed with 1 or more available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 99 261 830 111 285 886
Standard Deviation 8.0 6.8 18.3 8.8 17.8 57.8
CV% 8.1 2.6 2.2 7.9 6.2 6.5

Recovery for Mouse TRANCE/RANK L/TNFSF11 Quantikine ELISA Kit

The recovery of mouse TRANCE spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=6) 99 87-116
Serum (n=6) 93 80-104

Linearity

To assess the linearity of the assay, samples containing and/or spiked with mouse TRANCE were diluted with Calibrator Diluent and assayed.

Mouse TRANCE/TNFSF11/RANK L ELISA Linearity

Scientific Data Images for Mouse TRANCE/RANK L/TNFSF11 Quantikine ELISA Kit

Mouse TRANCE/TNFSF11/RANK L ELISA Standard Curve

Mouse TRANCE/TNFSF11/RANK L ELISA Standard Curve

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: TRANCE/TNFSF11/RANK L

TRANCE (TNF-related activation-induced cytokines), also known as RANK Ligand, OPGL, or ODF, is a TNF superfamily ligand produced by osteoblasts, bone marrow stromal cells, and activated T cells. It signals through RANK and is antagonized by the decoy receptor Osteoprotegerin. TRANCE stimulates bone resorption, is required for osteoclast differentiation, and is associated with the development of osteoporosis, vascular calcification, osteopetrosis, and progestin-induced breast cancer. It also mediates body temperature regulation and is essential for the development of a lactating mammary gland.

Long Name

TNF-related Activation-induced Cytokine

Alternate Names

CD254, ODF, OPGL, RANK L, RANKL, TNFSF11

Entrez Gene IDs

8600 (Human); 21943 (Mouse); 117516 (Rat); 102137946 (Cynomolgus Monkey)

Gene Symbol

TNFSF11

Additional TRANCE/TNFSF11/RANK L Products

Product Documents for Mouse TRANCE/RANK L/TNFSF11 Quantikine ELISA Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse TRANCE/RANK L/TNFSF11 Quantikine ELISA Kit

For research use only

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Mouse TRANCE/RANK L/TNFSF11 Quantikine ELISA Kit

Customer Reviews for Mouse TRANCE/RANK L/TNFSF11 Quantikine ELISA Kit (6)

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  • Mouse TRANCE/RANK L/TNFSF11 Quantikine ELISA Kit
    Name: Mary-Kate Hayward
    Sample Tested: Mammary gland tissue
    Verified Customer | Posted 04/05/2024
    Performed using normal mouse mammary tissue lysates.
  • Mouse TRANCE/RANK L/TNFSF11 Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Serum
    Verified Customer | Posted 10/21/2021
    Mouse TRANCE/RANK L/TNFSF11 Quantikine ELISA Kit MTR00
  • Mouse TRANCE/RANK L/TNFSF11 Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: ?TC-6 mouse beta cell insulinoma cell line
    Verified Customer | Posted 04/12/2018
  • Mouse TRANCE/RANK L/TNFSF11 Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: 7TD1 mouse hybridoma cell line
    Verified Customer | Posted 04/10/2018
  • Mouse TRANCE/RANK L/TNFSF11 Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: 4T1.2 mouse mammary carcinoma cells
    Verified Customer | Posted 03/25/2018
  • Mouse TRANCE/RANK L/TNFSF11 Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Cell culture supernatant
    Verified Customer | Posted 03/25/2018

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Protocols

View specific protocols for Mouse TRANCE/RANK L/TNFSF11 Quantikine ELISA Kit (MTR00):

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 50 µL Assay Diluent
  3.   Add 50 µL of Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  5.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.
    6. 100 µL Conjugate
  6.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate and wash 5 times.
    8. 100 µL Substrate Solution
  8.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
    9. 100 µL Stop Solution
  9.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

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