Mouse TRANCE/RANK L/TNFSF11 Quantikine ELISA Kit

  (42 citations)
(4 Reviews)
    
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Product Details
Assay Procedure
Citations (42)
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  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Serum (25 uL)
  • Sensitivity
    5 pg/mL
  • Assay Range
    31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum)
  • Specificity
    Free natural and recombinant mouse TRANCE
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
  • Interference
    Interference observed with 1 or more available related molecules.
Product Summary
The Quantikine Mouse TRANCE Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse TRANCE in cell culture supernates and serum. It contains NS0-expressed recombinant mouse TRANCE and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate recombinant mouse TRANCE accurately. Results obtained using natural mouse TRANCE showed dose response curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse TRANCE.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 99 261 830 111 285 886
Standard Deviation 8 6.8 18.3 8.8 17.8 57.8
CV% 8.1 2.6 2.2 7.9 6.2 6.5

Recovery

The recovery of mouse TRANCE spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=6) 99 87-116
Serum (n=6) 93 80-104
Linearity
To assess the linearity of the assay, samples containing and/or spiked with mouse TRANCE were diluted with Calibrator Diluent and assayed.
 TRANCE/TNFSF11/RANK L [HRP]
Product Datasheets

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Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: TRANCE/TNFSF11/RANK L
TRANCE (TNF-related activation-induced cytokines), also known as RANK Ligand, OPGL, or ODF, is a TNF superfamily ligand produced by osteoblasts, bone marrow stromal cells, and activated T cells. It signals through RANK and is antagonized by the decoy receptor Osteoprotegerin. TRANCE stimulates bone resorption, is required for osteoclast differentiation, and is associated with the development of osteoporosis, vascular calcification, osteopetrosis, and progestin-induced breast cancer. It also mediates body temperature regulation and is essential for the development of a lactating mammary gland.
  • Long Name:
    TNF-related Activation-induced Cytokine
  • Entrez Gene IDs:
    8600 (Human); 21943 (Mouse); 117516 (Rat)
  • Alternate Names:
    CD254 antigen; CD254; ODF; OPGL; OPGLOPTB2; Osteoclast differentiation factor; Osteoprotegerin ligand; RANK L; RANKL; RANKLreceptor activator of nuclear factor kappa B ligand; Receptor activator of nuclear factor kappa-B ligand; sOdf; TNF-related activation-induced cytokine; TNFSF11; TRANCE; TRANCEODFhRANKL2; tumor necrosis factor (ligand) superfamily, member 11; tumor necrosis factor ligand superfamily member 11
Related Research Areas
Assay Procedure
Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 50 µL Assay Diluent
  4.   Add 50 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.

  8. 100 µL Conjugate
  9.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  10.   Aspirate and wash 5 times.

  11. 100 µL Substrate Solution
  12.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

  13. 100 µL Stop Solution
  14.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

42 Citations: Showing 1 - 10
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Species
Sample Type
  1. Muramyl Dipeptide, a Shared Structural Motif of Peptidoglycans, Is a Novel Inducer of Bone Formation through Induction of Runx2
    Authors: OJ Park, J Kim, J Yang, CH Yun, SH Han
    J. Bone Miner. Res, 2017;0(0):.
    Species: Mouse
    Sample Type: Cell Culture Supernates
  2. Role of glucocorticoid-induced leucine zipper (GILZ) in inflammatory bone loss
    Authors: N Yang, B Baban, CM Isales, XM Shi
    PLoS ONE, 2017;12(8):e0181133.
    Species: Mouse
    Sample Type: Serum
  3. Disruption of the Cx43/miR21 pathway leads to osteocyte apoptosis and increased osteoclastogenesis with aging
    Authors: HM Davis, R Pacheco-Co, EG Atkinson, LR Brun, AR Gortazar, J Harris, M Hiasa, SA Bolarinwa, T Yoneda, M Ivan, A Bruzzaniti, T Bellido, LI Plotkin
    Aging Cell, 2017;0(0):.
    Species: Mouse
    Sample Type: Cell Culture Supernates
  4. Silicon Nitride: A Synthetic Mineral for Vertebrate Biology
    Sci Rep, 2016;6(0):31717.
    Species: Human
    Sample Type: Cell Culture Supernates
  5. 5-Azacytidine-induced protein 2 (AZI2) regulates bone mass by fine-tuning osteoclast survival.
    Authors: Maruyama K, Fukasaka M, Uematsu S, Takeuchi O, Kondo T, Saitoh T, Martino M, Akira S
    J Biol Chem, 2015;290(15):9377-86.
    Species: Mouse
    Sample Type: Serum
  6. The Proteasome Inhibitor Carfilzomib Suppresses Parathyroid Hormone-induced Osteoclastogenesis through a RANKL-mediated Signaling Pathway.
    Authors: Yang Y, Blair H, Shapiro I, Wang B
    J Biol Chem, 2015;290(27):16918-28.
    Species: Mouse
    Sample Type: Cell Culture Supernates
  7. A DNA segment spanning the mouse Tnfsf11 transcription unit and its upstream regulatory domain rescues the pleiotropic biologic phenotype of the RANKL null mouse.
    Authors: Onal M, Bishop K, St John H, Danielson A, Riley E, Piemontese M, Xiong J, Goellner J, O'Brien C, Pike J
    J Bone Miner Res, 2015;30(5):855-68.
    Species: Mouse
    Sample Type: Serum
  8. Porphyromonas gingivalis Stimulates Bone Resorption by Enhancing RANKL (Receptor Activator of NF-kappaB Ligand) through Activation of Toll-like Receptor 2 in Osteoblasts.
    Authors: Kassem A, Henning P, Lundberg P, Souza P, Lindholm C, Lerner U
    J Biol Chem, 2015;290(33):20147-58.
    Species: Mouse
    Sample Type: Tissue Homogenates
  9. Inhibition of bone remodeling in young mice by bisphosphonate displaces the plasma cell niche into the spleen.
    Authors: Teufel S, Grotsch B, Luther J, Derer A, Schinke T, Amling M, Schett G, Mielenz D, David J
    J Immunol, 2014;193(1):223-33.
    Species: Mouse
    Sample Type: Serum
  10. Effects of neuropeptides and mechanical loading on bone cell resorption in vitro.
    Authors: Yoo Y, Kwag J, Kim K, Kim C
    Int J Mol Sci, 2014;15(4):5874-83.
    Species: Mouse
    Sample Type: Cell Culture Supernates
  11. Bisphosphonates inhibit osteosarcoma-mediated osteolysis via attenuation of tumor expression of MCP-1 and RANKL.
    Authors: Ohba T, Cole H, Cates J, Slosky D, Haro H, Ando T, Schwartz H, Schoenecker J
    J Bone Miner Res, 2014;29(6):1431-45.
    Species: Mouse
    Sample Type: Cell Culture Supernates
  12. Autophagy in osteoblasts is involved in mineralization and bone homeostasis.
    Authors: Nollet M, Santucci-Darmanin S, Breuil V, Al-Sahlanee R, Cros C, Topi M, Momier D, Samson M, Pagnotta S, Cailleteau L, Battaglia S, Farlay D, Dacquin R, Barois N, Jurdic P, Boivin G, Heymann D, Lafont F, Lu S, Dempster D, Carle G, Pierrefite-Carle V
    Autophagy, 2014;10(11):1965-77.
    Species: Mouse
    Sample Type: Tissue Culture Supernates
  13. Sc65 is a novel endoplasmic reticulum protein that regulates bone mass homeostasis.
    Authors: Gruenwald K, Castagnola P, Besio R, Dimori M, Chen Y, Akel N, Swain F, Skinner R, Eyre D, Gaddy D, Suva L, Morello R
    J Bone Miner Res, 2014;29(3):666-75.
    Species: Mouse
    Sample Type: Serum
  14. Effects of the peroxisome proliferator-activated receptor (PPAR)-delta agonist GW501516 on bone and muscle in ovariectomized rats.
    Authors: Mosti M, Stunes A, Ericsson M, Pullisaar H, Reseland J, Shabestari M, Eriksen E, Syversen U
    Endocrinology, 2014;155(6):2178-89.
  15. Aconitum pseudo-laeve var. erectum inhibits receptor activator of nuclear factor kappa-B ligand-induced osteoclastogenesis via the c-Fos/nuclear factor of activated T-cells, cytoplasmic 1 signaling pathway and prevents lipopolysaccharide-induced bone loss in mice.
    Authors: Baek, Jong Min, Kim, Ju-Young, Cheon, Yoon-Hee, Park, Sun-Hyan, Ahn, Sung-Jun, Yoon, Kwon-Ha, Oh, Jaemin, Lee, Myeung S
    Molecules, 2014;19(8):11628-44.
    Species: Mouse
    Sample Type: Serum
  16. Increased IL-6 expression in osteoclasts is necessary but not sufficient for the development of Paget's disease of bone.
    Authors: Teramachi J, Zhou H, Subler M, Kitagawa Y, Galson D, Dempster D, Windle J, Kurihara N, Roodman G
    J Bone Miner Res, 2014;29(6):1456-65.
    Species: Mouse
    Sample Type: Cell Culture Supernates
  17. Resolvin E1 and chemokine-like receptor 1 mediate bone preservation.
    Authors: Gao L, Faibish D, Fredman G, Herrera B, Chiang N, Serhan C, Van Dyke T, Gyurko R
    J Immunol, 2013;190(2):689-94.
    Species: Mouse
    Sample Type: Cell Culture Supernates
  18. Soluble rank ligand produced by myeloma cells causes generalised bone loss in multiple myeloma.
    Authors: Buckle, Clive He, De Leenheer, Evy, Lawson, Michelle, Yong, Kwee, Rabin, Neil, Perry, Mark, Vanderkerken, Karen, Croucher, Peter Ia
    PLoS ONE, 2012;7(8):e41127.
    Species: Mouse
    Sample Type: Serum
  19. Receptor activator of nuclear factor kappaB ligand (RANKL) protein expression by B lymphocytes contributes to ovariectomy-induced bone loss.
    Authors: Onal M, Xiong J, Chen X, Thostenson J, Almeida M, Manolagas S
    Oncogene, 2012;287(35):29851-60.
    Species: Mouse
    Sample Type: Cell Culture Supernates
  20. Osteoprotection by semaphorin 3A.
    Authors: Hayashi M, Nakashima T, Taniguchi M, Kodama T, Kumanogoh A, Takayanagi H
    Nature, 2012;485(7396):69-74.
    Species: Mouse
    Sample Type: Serum
  21. The RANKL distal control region is required for the increase in RANKL expression, but not the bone loss, associated with hyperparathyroidism or lactation in adult mice.
    Authors: Onal M, Galli C, Fu Q, Xiong J, Weinstein RS, Manolagas SC, O'Brien CA
    Mol. Endocrinol., 2012;26(2):341-8.
    Species: Mouse
    Sample Type: Plasma
  22. Strontium fructose 1,6-diphosphate prevents bone loss in a rat model of postmenopausal osteoporosis via the OPG/RANKL/RANK pathway.
    Authors: Ma B, Zhang Q, Wu D, Wang YL, Hu YY, Cheng YP, Yang ZD, Zheng YY, Ying HJ
    Acta Pharmacol. Sin., 2012;33(4):479-89.
    Species: Rat
    Sample Type: Serum
  23. Effects of relaxin and estrogens on bone remodeling markers, receptor activator of NF-kB ligand (RANKL) and osteoprotegerin (OPG), in rat adjuvant-induced arthritis.
    Authors: Ho TY, Santora K, Chen JC, Frankshun AL, Bagnell CA
    Bone, 2011;48(6):1346-53.
    Species: Rat
    Sample Type: Plasma
  24. Mechanisms inducing low bone density in duchenne muscular dystrophy in mice and humans.
    Authors: Rufo A, Del Fattore A, Capulli M, Carvello F, De Pasquale L, Ferrari S, Pierroz D, Morandi L, De Simone M, Rucci N, Bertini E, Bianchi ML, De Benedetti F, Teti A
    J. Bone Miner. Res., 2011;26(8):1891-903.
    Species: Mouse
    Sample Type: Serum
  25. C17 prevents inflammatory arthritis and associated joint destruction in mice.
    Authors: Chao C, Joyce-Shaikh B, Grein J
    PLoS ONE, 2011;6(7):e22256.
    Species: Mouse
    Sample Type: Serum
  26. Increased trabecular bone formation in mice lacking the growth factor midkine.
    Authors: Neunaber C, Catala-Lehnen P, Beil FT
    J. Bone Miner. Res., 2010;25(8):1724-35.
    Species: Mouse
    Sample Type: Serum
  27. Systemic effects of ulna loading in male rats during functional adaptation.
    Authors: Sample SJ, Collins RJ, Wilson AP, Racette MA, Behan M, Markel MD, Kalscheur VL, Hao Z, Muir P
    J. Bone Miner. Res., 2010;25(9):2016-28.
    Species: Rat
    Sample Type: Plasma
  28. Beta-Arrestin2 regulates RANKL and ephrins gene expression in response to bone remodeling in mice.
    Authors: Pierroz DD, Rufo A, Bianchi EN, Glatt V, Capulli M, Rucci N, Cavat F, Rizzoli R, Teti A, Bouxsein ML, Ferrari SL
    J. Bone Miner. Res., 2009;24(5):775-84.
    Species: Mouse
    Sample Type: Cell Culture Supernates
  29. ADA-deficient SCID is associated with a specific microenvironment and bone phenotype characterized by RANKL/OPG imbalance and osteoblast insufficiency.
    Authors: Sauer AV, Mrak E, Hernandez RJ, Zacchi E, Cavani F, Casiraghi M, Grunebaum E, Roifman CM, Cervi MC, Ambrosi A, Carlucci F, Roncarolo MG, Villa A, Rubinacci A, Aiuti A
    Blood, 2009;114(15):3216-26.
    Species: Mouse
    Sample Type: Serum
  30. Mecp2 deficiency decreases bone formation and reduces bone volume in a rodent model of Rett syndrome.
    Authors: O'Connor RD, Zayzafoon M, Farach-Carson MC, Schanen NC
    Bone, 2009;45(2):346-56.
    Species: Mouse
    Sample Type: Serum
  31. Substance P stimulates bone marrow stromal cell osteogenic activity, osteoclast differentiation, and resorption activity in vitro.
    Authors: Wang L, Zhao R, Shi X, Wei T, Halloran BP, Clark DJ, Jacobs CR, Kingery WS
    Bone, 2009;45(2):309-20.
    Species: Mouse
    Sample Type: Cell Culture Supernates
  32. The essential role of IFN-gamma in the control of lethal Aggregatibacter actinomycetemcomitans infection in mice.
    Authors: Garlet GP, Cardoso CR, Campanelli AP, Garlet TP, Avila-Campos MJ, Cunha FQ, Silva JS
    Microbes Infect., 2008;10(5):489-96.
    Species: Mouse
    Sample Type: Tissue Homogenates
  33. Overexpression of bone sialoprotein leads to an uncoupling of bone formation and bone resorption in mice.
    Authors: Valverde P, Zhang J, Fix A, Zhu J, Ma W, Tu Q, Chen J
    J. Bone Miner. Res., 2008;23(11):1775-88.
    Species: Mouse
    Sample Type: Serum
  34. Comparison of the effects of genistein and zoledronic acid on the bone loss in OPG-deficient mice.
    Authors: Liu J, Xu K, Wen G, Guo H, Li S, Wu X, Dai R, Sheng Z, Liao E
    Bone, 2008;42(5):950-9.
    Species: Mouse
    Sample Type: Serum
  35. Osteoprotegerin inhibits vascular calcification without affecting atherosclerosis in ldlr(-/-) mice.
    Authors: Morony S, Tintut Y, Zhang Z, Cattley RC, Van G, Dwyer D, Stolina M, Kostenuik PJ, Demer LL
    Circulation, 2008;117(3):411-20.
    Species: Mouse
    Sample Type: Plasma
  36. Myeloma-derived Dickkopf-1 disrupts Wnt-regulated osteoprotegerin and RANKL production by osteoblasts: a potential mechanism underlying osteolytic bone lesions in multiple myeloma.
    Authors: Qiang YW, Chen Y, Stephens O, Brown N, Chen B, Epstein J, Barlogie B, Shaughnessy JD
    Blood, 2008;112(1):196-207.
    Species: Human
    Sample Type: Cell Culture Supernates
  37. Bradykinin potentiates cytokine-induced prostaglandin biosynthesis in osteoblasts by enhanced expression of cyclooxygenase 2, resulting in increased RANKL expression.
    Authors: Brechter AB, Lerner UH
    Arthritis Rheum., 2007;56(3):910-23.
    Species: Mouse
    Sample Type: Whole Tissue
  38. New 19-nor-(20S)-1alpha,25-dihydroxyvitamin D3 analogs strongly stimulate osteoclast formation both in vivo and in vitro.
    Authors: Sato M, Nakamichi Y, Nakamura M, Sato N, Ninomiya T, Muto A, Nakamura H, Ozawa H, Iwasaki Y, Kobayashi E, Shimizu M, DeLuca HF, Takahashi N, Udagawa N
    Bone, 2007;40(2):293-304.
    Species: Mouse
    Sample Type: Serum
  39. B cells and T cells are critical for the preservation of bone homeostasis and attainment of peak bone mass in vivo.
    Authors: Li Y, Toraldo G, Li A, Yang X, Zhang H, Qian WP, Weitzmann MN
    Blood, 2007;109(9):3839-48.
    Species: Mouse
    Sample Type: Cell Culture Supernates
  40. Attenuation of bone mass and increase of osteoclast formation in decoy receptor 3 transgenic mice.
    Authors: Tang CH, Hsu TL, Lin WW, Lai MZ, Yang RS, Hsieh SL, Fu WM
    J. Biol. Chem., 2007;282(4):2346-54.
    Species: Mouse
    Sample Type: Cell Culture Supernates
  41. Administration of cyclooxygenase-2 inhibitor reduces joint inflammation but exacerbates osteopenia in IL-1 alpha transgenic mice due to GM-CSF overproduction.
    Authors: Niki Y, Takaishi H, Takito J, Miyamoto T, Kosaki N, Matsumoto H, Toyama Y, Tada N
    J. Immunol., 2007;179(1):639-46.
    Species: Mouse
    Sample Type: Cell Culture Supernates
  42. Osteoprotegerin reduces the serum level of receptor activator of NF-kappaB ligand derived from osteoblasts.
    Authors: Nakamichi Y, Udagawa N, Kobayashi Y, Nakamura M, Yamamoto Y, Yamashita T, Mizoguchi T, Sato M, Mogi M, Penninger JM, Takahashi N
    J. Immunol., 2007;178(1):192-200.
    Species: Mouse
    Sample Type: Serum
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Sample Tested ?TC-6 mouse beta cell insulinoma cell line
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Sample Tested 7TD1 mouse hybridoma cell line
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Sample Tested 4T1.2 mouse mammary carcinoma cells
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