Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 88227
View all TRANCE/TNFSF11/RANK L Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse TRANCE
Arg72-Asp316
Accession # O35235

Specificity

Detects mouse TRANCE in direct ELISAs and Western blots. In direct ELISAs, shows 100% cross-reactivity with recombinant human (rh) TRANCE and no cross-reactivity with rhAPRIL, rhBAFF, rhCD27 Ligand, recombinant mouse (rm) CD27 Ligand, rhCD30 Ligand, rmCD30 Ligand, rhCD40 Ligand, rmEDA, rhFas Ligand, rmFas Ligand, rhGITR Ligand, rhTNF-alpha, rhTRAK, rmTSG, recombinant cotton rat TNF‑ alpha, rmTNF-alpha, recombinant porcine TNF-alpha, recombinant rat TNF-alpha, and rhVEGI.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for Mouse TRANCE/TNFSF11/RANK L Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Mouse TRANCE/TNFSF11/RANK L (Catalog # 462-TR)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TRANCE/TNFSF11/RANK L

TRANCE, also known as RANK Ligand, is a type II transmembrane protein belonging to the tumor necrosis factor superfamily and has been designated TNFSF11.

Long Name

TNF-related Activation-induced Cytokine

Alternate Names

CD254, ODF, OPGL, RANK L, RANKL, TNFSF11

Entrez Gene IDs

8600 (Human); 21943 (Mouse); 117516 (Rat); 102137946 (Cynomolgus Monkey)

Gene Symbol

TNFSF11

UniProt

O35235

Additional TRANCE/TNFSF11/RANK L Products

Product Documents for Mouse TRANCE/TNFSF11/RANK L Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse TRANCE/TNFSF11/RANK L Antibody

For research use only

Citations for Mouse TRANCE/TNFSF11/RANK L Antibody

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