Mouse TREM-1 PerCP-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects mouse TREM-1 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse TREM-2B, recombinant human (rh) TREM-1, or rhTREM-2 is observed.
  • Source
    Monoclonal Rat IgG2A Clone # 174031
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse TREM-1
    Accession # Q9JKE2
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    PerCP (Peridinin-chlorophyll Protein Complex)
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of TREM‑1 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse Gr‑1/Ly‑6G Alexa Fluor® 405‑conjugated Monoclonal Antibody(Catalog # FAB1037V) and either (A) Rat Anti-Mouse TREM‑1 PerCP‑conjugated Monoclonal Antibody (Catalog # FAB1187C) or (B) Rat IgG2A PerCP Isotype Control (Catalog # IC006C). View our protocol for Staining Membrane-associated Proteins
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: TREM-1

TREM-1 (Triggering Receptor Expressed on Myeloid cells) is a type I transmembrane protein having a single Ig-like domain. It associates with the adapter protein, DAP12, to deliver an activating signal. Several other TREM family members have been reported that are structurally similar but share less than 30% amino acid identity. TREM-1 is expressed on blood neutrophils and a subset of monocytes, and expression is up-regulated by bacterial LPS. Engagement of TREM-1 with a monoclonal antibody leads to expression of IL-8, MCP-1, and TNF-alpha suggesting that this receptor plays an important role in inflammatory responses. TREM-1 is expressed at high levels on neutrophils of patients with microbial sepsis and in mice with LPS-induced shock. Blockade of TREM-1 with a TREM-1/Fc fusion protein protected mice against LPS-induced shock.

  • References:
    1. Bouchon, A. (2000) J. Immunol. 164:4991.
    2. Bouchon, A. (2001) Nature 410:1103.
    3. Nathan, C. and A. Ding (2001) Nature Med. 7:530.
  • Long Name:
    Triggering Receptor Expressed on Myeloid Cells 1
  • Entrez Gene IDs:
    54210 (Human); 58217 (Mouse)
  • Alternate Names:
    CD354 antigen; CD354; TREM1; TREM-1Triggering receptor expressed on monocytes 1; triggering receptor expressed on myeloid cells 1; triggering-receptor TREM1
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2A PerCP‑conjugated Control Antibody

Ctrl IC006C
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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