TREM-3 is a type I transmembrane protein possessing a single extracellular Ig domain. It associates with the signal adapter molecule, DAP12, and delivers an activating signal. TREM-3 is expressed in macrophage cell lines and at low levels in T cell lines, but not in NK, B cell, or mast cell lines.
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 295502
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse TREM-3
Met1-Pro135 (predicted)
Accession # NP_067382
Met1-Pro135 (predicted)
Accession # NP_067382
Specificity
Detects mouse TREM-3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant mouse (rm) TREM-1 or rmTREM-2b is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Applications for Mouse TREM‑3 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Mouse TREM‑3
Sample: Recombinant Mouse TREM‑3
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: TREM-3
Long Name
Triggering Receptor Expressed on Myeloid Cells 3
Alternate Names
TREM3
Entrez Gene IDs
58218 (Mouse)
Gene Symbol
TREM3
UniProt
Additional TREM-3 Products
Product Documents for Mouse TREM‑3 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse TREM‑3 Antibody
For research use only
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
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- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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