Mouse TREML2/TLT-2 Antibody

Catalog #: AF6354 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF6354
AF6354-SP

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Detection of Mouse TREML2/TLT‑2 by Western Blot.

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Mouse TREML2/TLT-2 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse TREML2/TLT-2 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse (rm) TREML1, rmTREML4, rmTREM1, rmTREM2b, rmTREM3, rmTREM4, and recombinant human TREM2 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse TREML2/TLT-2
His25-Ala270
Accession # Q2LA85

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Flow Cytometry

2.5 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse TREML2/TLT‑2 by Western Blot. Western blot shows lysates of mouse spleen tissue. PVDF Membrane was probed with 1 µg/mL of Mouse TREML2/TLT-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6354) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A band was detected for TREML2/TLT-2 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Flow Cytometry

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Detection of TREML2/TLT-2 in M1 Mouse Cell Line by Flow Cytometry. M1 mouse myeloid leukemia cell line was stained with Mouse TREML2/TLT-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6354, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL010).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TREML2/TLT-2

Mouse Trem-like transcript 2 protein (TLT-2; also TREML2) is a 34 kDa (predicted for unglycosylated) type I transmembrane cell surface receptor and member of the Trem family of receptor proteins. Mouse TLT-2 is synthesized as a 329 amino acid (aa) precursor that contains a 24 aa signal sequence, a 246 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 38 aa cytoplasmic tail. Within the ECD are an Ig-like V-type domain (aa 25-126) and one potential site for N-linked glycosylation. Mature mouse TLT-2 shares 49% aa sequence identity with mature human TLT-2. Cell surface expression of TLT-2 is seen for most of the B and myeloid cell lines. TLT-2 is also expressed constitutively on CD8+ T cells.

Long Name

Triggering Receptor Expressed on Myeloid Cells-like 2

Entrez Gene IDs

79865 (Human); 328833 (Mouse)

Alternate Names

C6orf76; dJ238O23.1; FLJ13693; MGC149715; MGC149716; TLT2; TLT-2; TREML2; trem-like transcript 2 protein; triggering receptor expressed on myeloid cells-like 2; triggering receptor expressed on myeloid cells-like protein 2; UNQ6268/PRO20473

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