Detection of TREML4 in Mouse Splenocytes by Flow Cytometry. CD8+ CD11c+ mouse splenocytes were stained with Rat Anti-Mouse TREML4/TLT‑4 Monoclonal Antibody (Catalog # MAB5500, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
TREML4 (Triggering Receptor Expressed on Myeloid cells-Like 4), also known as TLT4, is a 53-55 kDa member of the Ig superfamily. It is likely expressed by leukocytes, and based on its structure, may act as an activating receptor. Mature mouse TREML4 is a type I transmembrane (TM) glycoprotein that is 235 amino acids (aa) in length. It contains a 171 aa extracellular region (aa 29-199) with a V-type Ig-like domain (aa 29-122); the TM segment contains one Lys residue. There is one splice variant that shows a deletion of aa 21-24, a segment that lies in the putative signal sequence. Rodent and human TREML4 are highly divergent, and thus over aa 25-195, mouse TREML4 shares 78% aa sequence identity with rat TREML4, but only 52% aa sequence identity with human TREML4.
Triggering Receptor Expressed on Myeloid Cells-like 4
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