Mouse TREML4/TLT-4 Antibody

Catalog # Availability Size / Price Qty
MAB5500
MAB5500-SP

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Detection of TREML4 in Mouse Splenocytes by Flow Cytometry.
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Mouse TREML4/TLT-4 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse TREML4/TLT-4 in ELISAs.
Source
Monoclonal Rat IgG2A Clone # 817914
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse TREML4/TLT-4
Gly25-Pro195
Accession # Q3LRV9
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of TREML4 antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of TREML4 in Mouse Splenocytes by Flow Cytometry. CD8+CD11c+mouse splenocytes were stained with Rat Anti-Mouse TREML4/TLT-4 Monoclonal Antibody (Catalog # MAB5500, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TREML4/TLT-4

TREML4 (Triggering Receptor Expressed on Myeloid cells-Like 4), also known as TLT4, is a 53-55 kDa member of the Ig superfamily.  It is likely expressed by leukocytes, and based on its structure, may act as an activating receptor.  Mature mouse TREML4 is a type I transmembrane (TM) glycoprotein that is 235 amino acids (aa) in length.  It contains a 171 aa extracellular region (aa 29-199) with a V-type Ig-like domain (aa 29-122); the TM segment contains one Lys residue.  There is one splice variant that shows a deletion of aa 21-24, a segment that lies in the putative signal sequence.  Rodent and human TREML4 are highly divergent, and thus over aa 25-195, mouse TREML4 shares 78% aa sequence identity with rat TREML4, but only 52% aa sequence identity with human TREML4.

Long Name
Triggering Receptor Expressed on Myeloid Cells-like 4
Entrez Gene IDs
285852 (Human); 224840 (Mouse); 680862 (Rat)
Alternate Names
TLT4; TREML4

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