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Mouse TROP-2 Alexa Fluor® 405-conjugated Antibody

Catalog #: AF1122V Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1122V-100UG

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Mouse TROP-2 Alexa Fluor® 405-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse TROP-2 in direct ELISAs and Western blots. In direct ELISAs, less than 30% cross-reactivity with recombinant human TROP‑2 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse TROP-2
Ser82-Thr268
Accession # Q8BGV3
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Label
Alexa Fluor 405 (Excitation= 405 nm, Emission= 421 nm)

Applications

Recommended Concentration
Sample
Western Blot
Optimal dilution of this antibody should be experimentally determined.
 
Flow Cytometry
Optimal dilution of this antibody should be experimentally determined.
 
Immunohistochemistry
Optimal dilution of this antibody should be experimentally determined.
 
CyTOF-ready
Optimal dilution of this antibody should be experimentally determined.
 
Immunocytochemistry
Optimal dilution of this antibody should be experimentally determined.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: TROP-2

TROP-2, also named tumor-associated calcium signal transducer 2 (TACSTD2), GA733 tumor associated antigen, and epithelial glycoprotein-1 (EGP-1), is a type I transmembrane protein highly expressed in carcinomas. It was originally identified as an antigen present on human gastrointestinal tumors and is the second of two members of this family.  The other family member is GA7332, also called EpCAM, TROP1, 171A, gp40 and KSA.  TROP2 can transduce an intracellular calcium signal and may play a role in tumor growth. It also has adhesive functions.

Long Name
Tumor-associated Calcium Signal Transducer 2
Entrez Gene IDs
4070 (Human); 56753 (Mouse)
Alternate Names
Cell surface glycoprotein Trop-2; EGP1; EGP-1; epithelial glycoprotein-1; GA733-1; GA733-1GP50; gastrointestinal tumor-associated antigen GA7331; gp50; M1S1cell surface glycoprotein TROP2; Membrane component chromosome 1 surface marker 1; membrane component, chromosome 1, surface marker 1; Pancreatic carcinoma marker protein GA733-1; pancreatic carcinoma marker protein GA7331,40kD glycoprotein, identified by monoclonal GA733; T16; TACSTD2; TROP2; TROP-2; TROP2GA7331; tumor-associated calcium signal transducer 2

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