Mouse TROP-2 Antibody

AF1122 8 Citations Datasheet / COA / SDS

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AF1122
AF1122-SP

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Detection of TROP‑2 in mIMCD-3 Mouse Cell Line by Flow Cytometry.

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Citations (8)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse TROP-2 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse TROP-2 in direct ELISAs and Western blots. In direct ELISAs, less than 30% cross-reactivity with recombinant human TROP‑2 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse TROP-2
Ser82-Thr268
Accession # Q8BGV3

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse TROP‑2

Flow Cytometry

0.25 µg/10⁶ cells

See below

Immunohistochemistry

5-15 µg/mL

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry

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Detection of TROP‑2 in mIMCD-3 Mouse Cell Line by Flow Cytometry. mIMCD-3 mouse epithelial cell line was stained with Goat Anti-Mouse TROP‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1122, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).

Immunocytochemistry

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TROP‑2 in XB2 Mouse Cell Line. TROP‑2 was detected in immersion fixed XB2 mouse teratoma keratinocyte cell line using Goat Anti-Mouse TROP‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1122) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry

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TROP‑2 in Mouse Skin. TROP‑2 was detected in immersion fixed paraffin-embedded sections of mouse skin using Goat Anti-Mouse TROP‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1122) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes in keratinocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TROP-2

TROP-2, also named tumor-associated calcium signal transducer 2 (TACSTD2), GA733 tumor associated antigen, and epithelial glycoprotein-1 (EGP-1), is a type I transmembrane protein highly expressed in carcinomas. It was originally identified as an antigen present on human gastrointestinal tumors and is the second of two members of this family. The other family member is GA7332, also called EpCAM, TROP1, 171A, gp40 and KSA. TROP2 can transduce an intracellular calcium signal and may play a role in tumor growth. It also has adhesive functions.

Long Name

Tumor-associated Calcium Signal Transducer 2

Entrez Gene IDs

4070 (Human); 56753 (Mouse)

Alternate Names

Cell surface glycoprotein Trop-2; EGP1; EGP-1; epithelial glycoprotein-1; GA733-1; GA733-1GP50; gastrointestinal tumor-associated antigen GA7331; gp50; M1S1cell surface glycoprotein TROP2; Membrane component chromosome 1 surface marker 1; membrane component, chromosome 1, surface marker 1; Pancreatic carcinoma marker protein GA733-1; pancreatic carcinoma marker protein GA7331,40kD glycoprotein, identified by monoclonal GA733; T16; TACSTD2; TROP2; TROP-2; TROP2GA7331; tumor-associated calcium signal transducer 2

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse TROP-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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Submucosal Gland Myoepithelial Cells Are Reserve Stem Cells That Can Regenerate Mouse Tracheal Epithelium
Authors: TJ Lynch, PJ Anderson, PG Rotti, SR Tyler, AK Crooke, SH Choi, DT Montoro, CL Silverman, W Shahin, R Zhao, CW Jensen-Cod, A Adamcakova, TIA Evans, W Xie, Y Zhang, H Mou, BP Herring, PS Thorne, J Rajagopal, C Yeaman, KR Parekh, JF Engelhardt
Cell Stem Cell, 2018;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC
YAP/TAZ-Dependent Reprogramming of Colonic Epithelium Links ECM Remodeling to Tissue Regeneration
Authors: S Yui, L Azzolin, M Maimets, MT Pedersen, RP Fordham, SL Hansen, HL Larsen, J Guiu, MRP Alves, CF Rundsten, JV Johansen, Y Li, CD Madsen, T Nakamura, M Watanabe, OH Nielsen, PJ Schweiger, S Piccolo, KB Jensen
Cell Stem Cell, 2018;22(1):35-49.e7.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Maternal Fat Feeding Augments Offspring Nephron Endowment in Mice
PLoS ONE, 2016;11(8):e0161578.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr
Pten Regulates Epithelial Cytodifferentiation during Prostate Development.
Authors: Lokody I, Francis J, Gardiner J, Erler J, Swain A
PLoS ONE, 2015;10(6):e0129470.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P
Nrf2 enhances cholangiocyte expansion in Pten-deficient livers.
Authors: Taguchi, Keiko, Hirano, Ikuo, Itoh, Tohru, Tanaka, Minoru, Miyajima, Atsushi, Suzuki, Akira, Motohashi, Hozumi, Yamamoto, Masayuki
Mol Cell Biol, 2014;34(5):900-13.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Hepatocytes, rather than cholangiocytes, can be the major source of primitive ductules in the chronically injured mouse liver.
Authors: Sekiya S, Suzuki A
Am J Pathol, 2014;184(5):1468-78.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Global quantification of tissue dynamics in the developing mouse kidney.
Authors: Short K, Combes A, Lefevre J, Ju A, Georgas K, Lamberton T, Cairncross O, Rumballe B, McMahon A, Hamilton N, Smyth I, Little M
Dev Cell, 2014;29(2):188-202.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Identification of TROP2 (TACSTD2), an EpCAM-like molecule, as a specific marker for TGF-beta1-dependent human epidermal Langerhans cells.
Authors: Eisenwort G, Jurkin J, Yasmin N, Bauer T, Gesslbauer B, Strobl H
J. Invest. Dermatol., 2011;131(10):2049-57.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry