Mouse TROP-2 Antibody

Catalog # Availability Size / Price Qty
AF1122
AF1122-SP
Detection of TROP‑2 in mIMCD-3 Mouse Cell Line by Flow Cytometry.
2 Images
Product Details
Citations (8)
FAQs
Supplemental Products
Reviews

Mouse TROP-2 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse TROP-2 in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant human TROP-2 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse TROP-2
Ser82-Thr268
Accession # Q8BGV3
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse TROP‑2
Flow Cytometry
0.25 µg/106 cells
See below
Immunohistochemistry
5-15 µg/mL
Perfusion fixed frozen sections of mouse testis
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry Detection of TROP‑2 in mIMCD-3 Mouse Cell Line by Flow Cytometry. View Larger

Detection of TROP‑2 in mIMCD-3 Mouse Cell Line by Flow Cytometry. mIMCD-3 mouse epithelial cell line was stained with Goat Anti-Mouse TROP‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1122, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).

Immunocytochemistry TROP‑2 in XB2 Mouse Cell Line. View Larger

TROP‑2 in XB2 Mouse Cell Line. TROP‑2 was detected in immersion fixed XB2 mouse teratoma keratinocyte cell line using Goat Anti-Mouse TROP‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1122) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TROP-2

TROP-2, also named tumor-associated calcium signal transducer 2 (TACSTD2), GA733 tumor associated antigen, and epithelial glycoprotein-1 (EGP-1), is a type I transmembrane protein highly expressed in carcinomas. It was originally identified as an antigen present on human gastrointestinal tumors and is the second of two members of this family.  The other family member is GA7332, also called EpCAM, TROP1, 171A, gp40 and KSA.  TROP2 can transduce an intracellular calcium signal and may play a role in tumor growth. It also has adhesive functions.

Long Name
Tumor-associated Calcium Signal Transducer 2
Entrez Gene IDs
4070 (Human); 56753 (Mouse)
Alternate Names
Cell surface glycoprotein Trop-2; EGP1; EGP-1; epithelial glycoprotein-1; GA733-1; GA733-1GP50; gastrointestinal tumor-associated antigen GA7331; gp50; M1S1cell surface glycoprotein TROP2; Membrane component chromosome 1 surface marker 1; membrane component, chromosome 1, surface marker 1; Pancreatic carcinoma marker protein GA733-1; pancreatic carcinoma marker protein GA7331,40kD glycoprotein, identified by monoclonal GA733; T16; TACSTD2; TROP2; TROP-2; TROP2GA7331; tumor-associated calcium signal transducer 2

Product Datasheets

Citations for Mouse TROP-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
Filter your results:

Filter by:

  1. Submucosal Gland Myoepithelial Cells Are Reserve Stem Cells That Can Regenerate Mouse Tracheal Epithelium
    Authors: TJ Lynch, PJ Anderson, PG Rotti, SR Tyler, AK Crooke, SH Choi, DT Montoro, CL Silverman, W Shahin, R Zhao, CW Jensen-Cod, A Adamcakova, TIA Evans, W Xie, Y Zhang, H Mou, BP Herring, PS Thorne, J Rajagopal, C Yeaman, KR Parekh, JF Engelhardt
    Cell Stem Cell, 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  2. YAP/TAZ-Dependent Reprogramming of Colonic Epithelium Links ECM Remodeling to Tissue Regeneration
    Authors: S Yui, L Azzolin, M Maimets, MT Pedersen, RP Fordham, SL Hansen, HL Larsen, J Guiu, MRP Alves, CF Rundsten, JV Johansen, Y Li, CD Madsen, T Nakamura, M Watanabe, OH Nielsen, PJ Schweiger, S Piccolo, KB Jensen
    Cell Stem Cell, 2018;22(1):35-49.e7.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Maternal Fat Feeding Augments Offspring Nephron Endowment in Mice
    PLoS ONE, 2016;11(8):e0161578.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Frozen
  4. Pten Regulates Epithelial Cytodifferentiation during Prostate Development.
    Authors: Lokody I, Francis J, Gardiner J, Erler J, Swain A
    PLoS ONE, 2015;10(6):e0129470.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Paraffin-embedded
  5. Nrf2 enhances cholangiocyte expansion in Pten-deficient livers.
    Authors: Taguchi, Keiko, Hirano, Ikuo, Itoh, Tohru, Tanaka, Minoru, Miyajima, Atsushi, Suzuki, Akira, Motohashi, Hozumi, Yamamoto, Masayuki
    Mol Cell Biol, 2014;34(5):900-13.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. Hepatocytes, rather than cholangiocytes, can be the major source of primitive ductules in the chronically injured mouse liver.
    Authors: Sekiya S, Suzuki A
    Am J Pathol, 2014;184(5):1468-78.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  7. Global quantification of tissue dynamics in the developing mouse kidney.
    Authors: Short K, Combes A, Lefevre J, Ju A, Georgas K, Lamberton T, Cairncross O, Rumballe B, McMahon A, Hamilton N, Smyth I, Little M
    Dev Cell, 2014;29(2):188-202.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Not Specified
  8. Identification of TROP2 (TACSTD2), an EpCAM-like molecule, as a specific marker for TGF-beta1-dependent human epidermal Langerhans cells.
    Authors: Eisenwort G, Jurkin J, Yasmin N, Bauer T, Gesslbauer B, Strobl H
    J. Invest. Dermatol., 2011;131(10):2049-57.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Mouse TROP-2 Antibody

There are currently no reviews for this product. Be the first to review Mouse TROP-2 Antibody and earn rewards!

Have you used Mouse TROP-2 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review