Mouse TROP-2 Antibody

(6 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse TROP-2 in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant human TROP-2 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse TROP-2
    Ser82-Thr268
    Accession # Q8BGV3
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse TROP‑2
  • Flow Cytometry
    0.25 µg/106 cells
    See below
  • Immunohistochemistry
    5-15 µg/mL
    Perfusion fixed frozen sections of mouse testis
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of TROP‑2 in mIMCD-3 Mouse Cell Line by Flow Cytometry. mIMCD-3 mouse epithelial cell line was stained with Goat Anti-Mouse TROP‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1122, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).
Immunocytochemistry
TROP‑2 in XB2 Mouse Cell Line. TROP‑2 was detected in immersion fixed XB2 mouse teratoma keratinocyte cell line using Goat Anti-Mouse TROP‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1122) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TROP-2

TROP-2, also named tumor-associated calcium signal transducer 2 (TACSTD2), GA733 tumor associated antigen, and epithelial glycoprotein-1 (EGP-1), is a type I transmembrane protein highly expressed in carcinomas. It was originally identified as an antigen present on human gastrointestinal tumors and is the second of two members of this family.  The other family member is GA7332, also called EpCAM, TROP1, 171A, gp40 and KSA.  TROP2 can transduce an intracellular calcium signal and may play a role in tumor growth. It also has adhesive functions.

  • Long Name:
    Tumor-associated Calcium Signal Transducer 2
  • Entrez Gene IDs:
    4070 (Human); 56753 (Mouse)
  • Alternate Names:
    Cell surface glycoprotein Trop-2; EGP1; EGP-1; epithelial glycoprotein-1; GA733-1; GA733-1GP50; gastrointestinal tumor-associated antigen GA7331; gp50; M1S1cell surface glycoprotein TROP2; Membrane component chromosome 1 surface marker 1; membrane component, chromosome 1, surface marker 1; Pancreatic carcinoma marker protein GA733-1; pancreatic carcinoma marker protein GA7331,40kD glycoprotein, identified by monoclonal GA733; T16; TACSTD2; TROP2; TROP-2; TROP2GA7331; tumor-associated calcium signal transducer 2
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Species
Applications
Sample Type
  1. Maternal Fat Feeding Augments Offspring Nephron Endowment in Mice
    PLoS ONE, 2016;11(8):e0161578.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  2. Pten Regulates Epithelial Cytodifferentiation during Prostate Development.
    Authors: Lokody I, Francis J, Gardiner J, Erler J, Swain A
    PLoS ONE, 2015;10(6):e0129470.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  3. Hepatocytes, rather than cholangiocytes, can be the major source of primitive ductules in the chronically injured mouse liver.
    Authors: Sekiya S, Suzuki A
    Am J Pathol, 2014;184(5):1468-78.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  4. Global quantification of tissue dynamics in the developing mouse kidney.
    Authors: Short K, Combes A, Lefevre J, Ju A, Georgas K, Lamberton T, Cairncross O, Rumballe B, McMahon A, Hamilton N, Smyth I, Little M
    Dev Cell, 2014;29(2):188-202.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Not Specified
  5. Nrf2 enhances cholangiocyte expansion in Pten-deficient livers.
    Authors: Taguchi, Keiko, Hirano, Ikuo, Itoh, Tohru, Tanaka, Minoru, Miyajima, Atsushi, Suzuki, Akira, Motohashi, Hozumi, Yamamoto, Masayuki
    Mol Cell Biol, 2014;34(5):900-13.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  6. Identification of TROP2 (TACSTD2), an EpCAM-like molecule, as a specific marker for TGF-beta1-dependent human epidermal Langerhans cells.
    Authors: Eisenwort G, Jurkin J, Yasmin N, Bauer T, Gesslbauer B, Strobl H
    J. Invest. Dermatol., 2011;131(10):2049-57.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
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Isotype Controls
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