Mouse TROP-2 Antibody

• Species Reactivity
  Mouse
• Specificity
  Detects mouse TROP-2 in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant human TROP-2 is observed.
• Source
  Polyclonal Goat IgG
• Purification
  Antigen Affinity-purified
• Immunogen
  Mouse myeloma cell line NS0-derived recombinant mouse TROP-2
  Ser82-Thr268
  Accession # Q8BGV3
• Formulation
  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Label
  Unconjugated

• Western Blot
  0.1 µg/mL
  Recombinant Mouse TROP‑2
• Flow Cytometry
  0.25 µg/10⁶ cells
  See below
• Immunohistochemistry
  5-15 µg/mL
  Perfusion fixed frozen sections of mouse testis
• CyTOF-ready
  Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
• Immunocytochemistry
  5-15 µg/mL
  See below

Flow Cytometry click image to view larger

Detection of TROP‑2 in mIMCD-3 Mouse Cell Line by Flow Cytometry. mIMCD-3 mouse epithelial cell line was stained with Goat Anti-Mouse TROP‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1122, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).

Immunocytochemistry click image to view larger

TROP‑2 in XB2 Mouse Cell Line. TROP‑2 was detected in immersion fixed XB2 mouse teratoma keratinocyte cell line using Goat Anti-Mouse TROP‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1122) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reconstitution
  Reconstitute at 0.2 mg/mL in sterile PBS.

  Reconstitution Buffer Available
• Shipping
  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
• Stability & Storage
  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

• Long Name:
  Tumor-associated Calcium Signal Transducer 2

• Entrez Gene IDs:
  4070 (Human); 56753 (Mouse)

• Alternate Names:
  Cell surface glycoprotein Trop-2; EGP1; EGP-1; epithelial glycoprotein-1; GA733-1; GA733-1GP50; gastrointestinal tumor-associated antigen GA7331; gp50; M1S1cell surface glycoprotein TROP2; Membrane component chromosome 1 surface marker 1; membrane component, chromosome 1, surface marker 1; Pancreatic carcinoma marker protein GA733-1; pancreatic carcinoma marker protein GA7331,40kD glycoprotein, identified by monoclonal GA733; T16; TACSTD2; TROP2; TROP-2; TROP2GA7331; tumor-associated calcium signal transducer 2

FAQs

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