Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry-Paraffin, Western Blot, ELISA Development

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 263019
View all Tryptase beta-1/MCP-7 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Tryptase beta ‑1/MCP‑7/Mcpt7
Ile29-Phe273
Accession # Q02844

Specificity

Detects mouse Tryptase beta ‑1/MCP‑7/Mcpt7 in direct ELISAs and Western blots. In direct ELISAs, 100% cross‑reactivity with recombinant human (rh) Tryptase alpha and rhTryptase beta ‑2 is observed and no cross-reactivity with recombinant mouse MCPT-1 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for Mouse Tryptase beta-1/MCP-7/Mcpt7 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Mouse Tryptase  beta ‑1/MCP‑7/Mcpt7 (Catalog # 1937-SE)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Tryptase beta-1/MCP-7

Tryptase beta -1 is a serine protease with trypsin-like activity, which is sometimes also referred to as Mast Cell Protease 7 (MCP-7 or Mcpt7) (1). It is stored in the secretory granules of mouse mast cells (2). It exhibits anticoagulant activity due to its ability to degrade fibrinogen in the presence of the diverse array of protease inhibitors in plasma (3). The deduced amino acid (aa) sequence for mouse Tryptase beta -1 consists of a signal peptide (aa 1‑18), a pro region (aa 19‑28) and the mature chain (aa 29‑273).

References

  1. McNeil, H.P. et al. (1992) Proc. Natl. Acad. Sci. USA 89:11174.
  2. Matsumoto, R et al. (1995) J. Biol. Chem. 270:19524.
  3. Huang, C. et al. (1997) J. Biol. Chem. 272:31885.

Long Name

Mast Cell Protease 7

Alternate Names

Mast Cell Tryptase, MCP-7, Mcp7, Mcpt7, TPSB1, TRYB1, Tryptase beta1

Entrez Gene IDs

100503895 (Mouse)

Gene Symbol

TPSAB1

UniProt

Q02844

Additional Tryptase beta-1/MCP-7 Products

Product Documents for Mouse Tryptase beta-1/MCP-7/Mcpt7 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Tryptase beta-1/MCP-7/Mcpt7 Antibody

For research use only

Citations for Mouse Tryptase beta-1/MCP-7/Mcpt7 Antibody

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