Mouse TSLP Antibody

Name: Mouse TSLP Antibody
Brand: R&D Systems

(3 citations)

Datasheet / CoA / SDS

Neutralization

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Product Details

Citations (3)

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Species Reactivity

Mouse
Specificity

Detects mouse TSLP in direct ELISAs and Western blots.
Source

Polyclonal Goat IgG
Purification

Antigen Affinity-purified
Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse TSLP
Tyr20-Glu140
Accession # Q9JIE6
Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.
Label

Unconjugated

Product Datasheets

Product Datasheet

COA

Certificate of Analysis Lookup

Certificate of Analysis Request Form

SDS

Applications

Recommended
Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse TSLP (Catalog # 555-TS)

Neutralization

Measured by its ability to neutralize TSLP-induced proliferation in the BaF3 mouse pro‑B cell line transfected with mouse IL‑7 R alpha /CD127 [Park, L.S. et al. (2000) J. Exp. Med. 192:659]. The Neutralization Dose (ND₅₀) is typically 0.1-0.4 µg/mL in the presence of 7.5 ng/mL Recombinant Mouse TSLP.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Neutralization

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Cell Proliferation Induced by TSLP and Neutralization by Mouse TSLP Antibody. Recombinant Mouse TSLP (Catalog # 555-TS) stimulates proliferation in the BaF3 mouse pro‑B cell line transfected with mouse IL‑7 R alpha /CD127 in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse TSLP (7.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse TSLP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF555). The ND₅₀ is typically 0.1-0.4 µg/mL.

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TSLP

Stromal Lymphopoietin (TSLP) was originally identified from the conditioned medium of a mouse thymic stromal cell line as a protein that promoted the development of B cells. The activity of mouse TSLP overlaps with, but is distinct from, that of mouse IL-7 (1). Mouse TSLP cDNA encodes a 140 amino acid (aa) residue precursor protein with a 19 aa signal sequence. Within the mature region, there are three potential N-glycosylation sites. The Sf 21 cell expressed rmTSLP is likely to be glycosylated at all three sites, as three major glycoforms were visible on SDS-PAGE (Figure 1). Insect cells are known to express relatively simple and homogeneous N-glycans that mainly belong to the high mannose type (2). This recombinant protein was found to be an excellent substrate for N-specific glycosidases such as Endo F3 (Figure 1). The majority of the glycans on rmTSLP can be readily removed by Endo F3. However, a small percentage of the glycans is somewhat resistant to Endo F3 digestion, possibly lacking core fucose, as it is known that core fucosylated N-glycans are strongly preferred substrates for Endo F3 digestion (3).

References:
1. Sims, J.E. et al. (2000) J. Exp. Med. 192:671.
2. Staudacher, E. et al. (1992) Eur. J. Biochem. 207:987.
3. Tarentino, A.L. and T.H. Jr. Plummer (1994) Glycobiology 4: 771.

Long Name:

Thymic Stromal Lymphopoietin

Entrez Gene IDs:

85480 (Human); 53603 (Mouse)

Alternate Names:

thymic stromal lymphopoietin; TSLP

Related Research Areas

Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Species	Applications	Sample Type
Thymic stromal lymphopoietin (TSLP)-mediated dermal inflammation aggravates experimental asthma. Authors: Han H, Xu W, Headley MB Mucosal Immunol, 2012;5(3):342-51. Species: Mouse Sample Type: In Vivo Application: In vivo Thymic stromal lymphopoietin contributes to myeloid hyperplasia and increased immunoglobulins, but not epidermal hyperplasia, in RabGEF1-deficient mice. Authors: Tsai M, Chen CC, Mukai K Am. J. Pathol., 2010;177(5):2411-20. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded A potential role of thymic stromal lymphopoietin in the recruitment of macrophages to mouse intervertebral disc cells via monocyte chemotactic protein 1 induction: implications for herniated discs. Authors: Ohba T, Haro H, Ando T, Koyama K, Hatsushika K, Suenaga F, Ohnuma Y, Nakamura Y, Katoh R, Ogawa H, Hamada Y, Nakao A Arthritis Rheum., 2008;58(11):3510-9. Species: Mouse Sample Type: Whole Cells Application: Neut

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Isotype Controls

Description Application Cat# Citations Images

Normal Goat IgG Control

Ctrl AB-108-C 203

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Mouse TSLP Antibody

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Certificate of Analysis Lookup

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FAQs

Normal Goat IgG Control

Reconstitution Buffer 1 (PBS)

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