Mouse TSLP Antibody

Catalog # Availability Size / Price Qty
AF555
AF555-SP
Cell Proliferation Induced by TSLP and Neutralization by Mouse TSLP Antibody.
1 Image
Product Details
Citations (3)
FAQs
Supplemental Products
Reviews

Mouse TSLP Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse TSLP in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse TSLP
Tyr20-Glu140
Accession # Q9JIE6
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse TSLP (Catalog # 555-TS)
Neutralization
Measured by its ability to neutralize TSLP-induced proliferation in the BaF3 mouse pro‑B cell line transfected with mouse IL‑7 R alpha /CD127 [Park, L.S. et al. (2000) J. Exp. Med. 192:659]. The Neutralization Dose (ND50) is typically 0.1-0.4 µg/mL in the presence of 7.5 ng/mL Recombinant Mouse TSLP.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Neutralization Cell Proliferation Induced by TSLP and Neutralization by Mouse TSLP Antibody. View Larger

Cell Proliferation Induced by TSLP and Neutralization by Mouse TSLP Antibody. Recombinant Mouse TSLP (Catalog # 555-TS) stimulates proliferation in the BaF3 mouse pro‑B cell line transfected with mouse IL‑7 R alpha /CD127 in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse TSLP (7.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse TSLP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF555). The ND50 is typically 0.1-0.4 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TSLP

Stromal Lymphopoietin (TSLP) was originally identified from the conditioned medium of a mouse thymic stromal cell line as a protein that promoted the development of B cells. The activity of mouse TSLP overlaps with, but is distinct from, that of mouse IL-7 (1). Mouse TSLP cDNA encodes a 140 amino acid (aa) residue precursor protein with a 19 aa signal sequence. Within the mature region, there are three potential N-glycosylation sites. The Sf 21 cell expressed rmTSLP is likely to be glycosylated at all three sites, as three major glycoforms were visible on SDS-PAGE (Figure 1). Insect cells are known to express relatively simple and homogeneous N-glycans that mainly belong to the high mannose type (2). This recombinant protein was found to be an excellent substrate for N-specific glycosidases such as Endo F3 (Figure 1). The majority of the glycans on rmTSLP can be readily removed by Endo F3. However, a small percentage of the glycans is somewhat resistant to Endo F3 digestion, possibly lacking core fucose, as it is known that core fucosylated N-glycans are strongly preferred substrates for Endo F3 digestion (3).

References
  1. Sims, J.E. et al. (2000) J. Exp. Med. 192:671.
  2. Staudacher, E. et al. (1992) Eur. J. Biochem. 207:987.
  3. Tarentino, A.L. and T.H. Jr. Plummer (1994) Glycobiology 4: 771.
Long Name
Thymic Stromal Lymphopoietin
Entrez Gene IDs
85480 (Human); 53603 (Mouse)
Alternate Names
thymic stromal lymphopoietin; TSLP

Product Datasheets

Citations for Mouse TSLP Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
Filter your results:

Filter by:

  1. Thymic stromal lymphopoietin (TSLP)-mediated dermal inflammation aggravates experimental asthma.
    Authors: Han H, Xu W, Headley MB
    Mucosal Immunol, 2012;5(3):342-51.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In vivo
  2. Thymic stromal lymphopoietin contributes to myeloid hyperplasia and increased immunoglobulins, but not epidermal hyperplasia, in RabGEF1-deficient mice.
    Authors: Tsai M, Chen CC, Mukai K
    Am. J. Pathol., 2010;177(5):2411-20.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Paraffin-embedded
  3. A potential role of thymic stromal lymphopoietin in the recruitment of macrophages to mouse intervertebral disc cells via monocyte chemotactic protein 1 induction: implications for herniated discs.
    Authors: Ohba T, Haro H, Ando T, Koyama K, Hatsushika K, Suenaga F, Ohnuma Y, Nakamura Y, Katoh R, Ogawa H, Hamada Y, Nakao A
    Arthritis Rheum., 2008;58(11):3510-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neut

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Mouse TSLP Antibody

There are currently no reviews for this product. Be the first to review Mouse TSLP Antibody and earn rewards!

Have you used Mouse TSLP Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review