Mouse TSLP Antibody
Mouse TSLP Antibody Summary
Tyr20-Glu140
Accession # Q9JIE6
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
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Cell Proliferation Induced by TSLP and Neutralization by Mouse TSLP Antibody. Recombinant Mouse TSLP (Catalog # 555-TS) stimulates proliferation in the BaF3 mouse pro‑B cell line transfected with mouse IL‑7 R alpha /CD127 in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse TSLP (7.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse TSLP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF555). The ND50 is typically 0.1-0.4 µg/mL.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TSLP
Stromal Lymphopoietin (TSLP) was originally identified from the conditioned medium of a mouse thymic stromal cell line as a protein that promoted the development of B cells. The activity of mouse TSLP overlaps with, but is distinct from, that of mouse IL-7 (1). Mouse TSLP cDNA encodes a 140 amino acid (aa) residue precursor protein with a 19 aa signal sequence. Within the mature region, there are three potential N-glycosylation sites. The Sf 21 cell expressed rmTSLP is likely to be glycosylated at all three sites, as three major glycoforms were visible on SDS-PAGE (Figure 1). Insect cells are known to express relatively simple and homogeneous N-glycans that mainly belong to the high mannose type (2). This recombinant protein was found to be an excellent substrate for N-specific glycosidases such as Endo F3 (Figure 1). The majority of the glycans on rmTSLP can be readily removed by Endo F3. However, a small percentage of the glycans is somewhat resistant to Endo F3 digestion, possibly lacking core fucose, as it is known that core fucosylated N-glycans are strongly preferred substrates for Endo F3 digestion (3).
- Sims, J.E. et al. (2000) J. Exp. Med. 192:671.
- Staudacher, E. et al. (1992) Eur. J. Biochem. 207:987.
- Tarentino, A.L. and T.H. Jr. Plummer (1994) Glycobiology 4: 771.
Product Datasheets
Citations for Mouse TSLP Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Thymic stromal lymphopoietin (TSLP)-mediated dermal inflammation aggravates experimental asthma.
Authors: Han H, Xu W, Headley MB
Mucosal Immunol, 2012;5(3):342-51.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
Thymic stromal lymphopoietin contributes to myeloid hyperplasia and increased immunoglobulins, but not epidermal hyperplasia, in RabGEF1-deficient mice.
Authors: Tsai M, Chen CC, Mukai K
Am. J. Pathol., 2010;177(5):2411-20.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
A potential role of thymic stromal lymphopoietin in the recruitment of macrophages to mouse intervertebral disc cells via monocyte chemotactic protein 1 induction: implications for herniated discs.
Authors: Ohba T, Haro H, Ando T, Koyama K, Hatsushika K, Suenaga F, Ohnuma Y, Nakamura Y, Katoh R, Ogawa H, Hamada Y, Nakao A
Arthritis Rheum., 2008;58(11):3510-9.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization
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