Measured by its ability to neutralize TSLP-induced proliferation in the BaF3 mouse pro‑B cell line transfected with mouse IL‑7 R alpha /CD127 [Park, L.S. et al. (2000) J. Exp. Med. 192:659]. The Neutralization Dose (ND50) is typically 0.1-0.4 µg/mL in the presence of 7.5 ng/mL Recombinant Mouse TSLP.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Cell Proliferation Induced by TSLP and Neutralization by Mouse TSLP Antibody. Recombinant Mouse TSLP (Catalog # 555-TS) stimulates proliferation in the BaF3 mouse pro‑B cell line transfected with mouse IL‑7 R alpha /CD127 in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse TSLP (7.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse TSLP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF555). The ND50 is typically 0.1-0.4 µg/mL.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Stromal Lymphopoietin (TSLP) was originally identified from the conditioned medium of a mouse thymic stromal cell line as a protein that promoted the development of B cells. The activity of mouse TSLP overlaps with, but is distinct from, that of mouse IL-7 (1). Mouse TSLP cDNA encodes a 140 amino acid (aa) residue precursor protein with a 19 aa signal sequence. Within the mature region, there are three potential N-glycosylation sites. The Sf 21 cell expressed rmTSLP is likely to be glycosylated at all three sites, as three major glycoforms were visible on SDS-PAGE (Figure 1). Insect cells are known to express relatively simple and homogeneous N-glycans that mainly belong to the high mannose type (2). This recombinant protein was found to be an excellent substrate for N-specific glycosidases such as Endo F3 (Figure 1). The majority of the glycans on rmTSLP can be readily removed by Endo F3. However, a small percentage of the glycans is somewhat resistant to Endo F3 digestion, possibly lacking core fucose, as it is known that core fucosylated N-glycans are strongly preferred substrates for Endo F3 digestion (3).
Sims, J.E. et al. (2000) J. Exp. Med. 192:671.
Staudacher, E. et al. (1992) Eur. J. Biochem. 207:987.
Tarentino, A.L. and T.H. Jr. Plummer (1994) Glycobiology 4: 771.
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