Mouse TSLP Quantikine ELISA Kit

(10 citations)   
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
  • Sensitivity
    6.3 pg/mL
  • Assay Range
    7.8 - 500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
  • Specificity
    Natural and recombinant mouse TSLP
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Product Summary
The Quantikine Mouse TSLP Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse TSLP in cell culture supernates, mouse serum, and plasma. It contains NS0-expressed recombinant mouse TSLP and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse TSLP showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse TSLP.

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Standard Deviation2.


The recovery of mouse TSLP spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=6) 103 93-114
EDTA Plasma (n=4) 94 80-108
Heparin Plasma (n=5) 96 89-119
Serum (n=5) 92 87-101
To assess the linearity of the assay, samples spiked with high concentrations of mouse TSLP in each matrix were diluted with Calibrator Diluent and then assayed.
Mouse TSLP Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: TSLP
Thymic Stromal Lymphopoietin (TSLP) was originally identified as an activity from the conditioned medium of a mouse thymic stromal cell line that promoted the development of B cells. The activities of mouse TSLP overlap with, but are distinct from, those of mouse IL-7. Both mouse TSLP and IL-7 can co-stimulate growth of thymocytes and mature T cells, and support B lymphopoiesis in long-term cultures of fetal liver cells and bone-marrow cells. Whereas mouse IL-7 facilitates the development of B220+/IgM- pre-B cells, mouse TSLP promotes the development B220+/IgM+ B cells. Human TSLP was reported to preferentially stimulate myeloid cells, inducing the release of T cell-attracting chemokines from monocytes and enhancing the maturation of CD11c+ dendritic cells. Human TSLP cDNA encodes a 159 amino acid (aa) residue precursor protein with a 28 aa signal sequence. Within the mature region, six of the seven cysteine residues present in the mouse TSLP involved in intramolecular disulfide bond formation are conserved in the human TSLP. Human TSLP shares approximately 43% aa sequence identity with mouse TSLP. By Northern blot analysis, human TSLP expression has been detected in many tissues with the highest expressions in heart, liver, testis and prostate. TSLP signals through a heterodimeric receptor complex that consists of IL-7 R alpha and the TSLP R, a member of the hemopoietin receptor family most closely related to Rgammac.

TSLP R, also named Delta and CRLM-2 (Cytokine Receptor-like Module-2), was originally cloned as a novel type 1 cytokine receptor with similarity to the common gamma chain (gamma c). It was subsequently identified as a subunit of the cellular receptor for the IL-7-like cytokine TSLP and termed TSLP R. TSLP R shows 24% sequence identity with the mouse gc receptor. TSLP R expression is constitutive in immune and hematopoietic cells, but is up-regulated in Th2 cells.

    • Long Name
      Thymic Stromal Lymphopoietin
    • Entrez Gene IDs
      85480 (Human); 53603 (Mouse);
    • Alternate Names
      thymic stromal lymphopoietin;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 50 µL Assay Diluent
    4.   Add 50 µL of Assay Diluent to each well.

    5. 50 µL Standard, Control, or Sample
    6.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
    7.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.

    8. 100 µL Conjugate
    9.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
    10.   Aspirate and wash 5 times.

    11. 100 µL Substrate Solution
    12.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

    13. 100 µL Stop Solution
    14.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    Showing Results 1 - 10 of 10
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    Sample Type
    1. Innate sensing of microbial products promotes wound-induced skin cancer.
      Authors: Hoste E, Arwert E, Lal R, South A, Salas-Alanis J, Murrell D, Donati G, Watt F
      Nat Commun, 2015;6(0):5932.
      Species: Mouse
      Sample Type: Serum
    2. IL-22 Is Produced by Innate Lymphoid Cells and Limits Inflammation in Allergic Airway Disease.
      Authors: Taube C, Tertilt C, Gyulveszi G, Dehzad N, Kreymborg K, Schneeweiss K, Michel E, Reuter S, Renauld JC, Arnold-Schild D, Schild H, Buhl R, Becher B
      PLoS ONE, 2011;6(7):e21799.
      Species: Mouse
      Sample Type: BALF
    3. Thymic stromal lymphopoietin is produced by dendritic cells.
      Authors: Kashyap M, Rochman Y, Spolski R
      J. Immunol., 2011;187(3):1207-11.
      Species: Mouse
      Sample Type: BALF
    4. IL-33-responsive lineage- CD25+ CD44(hi) lymphoid cells mediate innate type 2 immunity and allergic inflammation in the lungs.
      Authors: Bartemes KR, Iijima K, Kobayashi T, Kephart GM, McKenzie AN, Kita H
      J. Immunol., 2011;188(3):1503-13.
      Species: Mouse
      Sample Type: BALF
    5. Thymic stromal lymphopoietin contributes to myeloid hyperplasia and increased immunoglobulins, but not epidermal hyperplasia, in RabGEF1-deficient mice.
      Authors: Tsai M, Chen CC, Mukai K
      Am. J. Pathol., 2010;177(5):2411-20.
      Species: Mouse
      Sample Type: Tissue Homogenates
    6. A small molecule CRTH2 antagonist inhibits FITC-induced allergic cutaneous inflammation.
      Authors: Boehme SA, Franz-Bacon K, Chen EP, Sasik R, Sprague LJ, Ly TW, Hardiman G, Bacon KB
      Int. Immunol., 2009;21(1):81-93.
      Species: Mouse
      Sample Type: Tissue Homogenates
    7. Skin-derived TSLP triggers progression from epidermal-barrier defects to asthma.
      Authors: Demehri S, Morimoto M, Holtzman MJ
      PLoS Biol., 2009;7(5):e1000067.
      Species: Mouse
      Sample Type: Serum
    8. Thymic stromal lymphopoietin is a critical mediator of IL-13-driven allergic inflammation.
      Authors: Miyata M, Nakamura Y, Shimokawa N, Ohnuma Y, Katoh R, Matsuoka S, Okumura K, Ogawa H, Masuyama K, Nakao A
      Eur. J. Immunol., 2009;39(11):3078-83.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    9. Epidermal Notch1 loss promotes skin tumorigenesis by impacting the stromal microenvironment.
      Authors: Demehri S, Turkoz A, Kopan R
      Cancer Cell, 2009;16(1):55-66.
      Species: Mouse
      Sample Type: Serum
    10. A potential role of thymic stromal lymphopoietin in the recruitment of macrophages to mouse intervertebral disc cells via monocyte chemotactic protein 1 induction: implications for herniated discs.
      Authors: Ohba T, Haro H, Ando T, Koyama K, Hatsushika K, Suenaga F, Ohnuma Y, Nakamura Y, Katoh R, Ogawa H, Hamada Y, Nakao A
      Arthritis Rheum., 2008;58(11):3510-9.
      Species: Mouse
      Sample Type: Cell Culture Supernates
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