TSLP (Thymic Stromal Lymphopoietin) is a secreted cytokine that signals through a complex of TSLP R and IL-7 R alpha/CD127. It acts on thymic dendritic cells which convert potentially autoreactive T cells into regulatory T cells. TSLP promotes fetal liver pro-B cell proliferation as well as large pre-B cell differentiation and B cell lymphopoiesis in adult bone marrow. It is produced by allergen challenged keratinocytes and mucosal epithelial cells. It enhances Th2 biased allergic inflammation by limiting Th1/Th17 inflammation, promoting Th2 differentiation, and enhancing the function of basophils, eosinophils, and mast cells.
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Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL)
Sensitivity
6.3 pg/mL
Assay Range
7.8-500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
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Product Summary for Mouse TSLP Quantikine ELISA Kit
The Quantikine Mouse TSLP Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse TSLP in cell culture supernates, mouse serum, and plasma. It contains NS0-expressed recombinant mouse TSLP and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse TSLP showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse TSLP.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Species
Mouse
Specificity
Natural and recombinant mouse TSLP
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 31 | 31 | 31 |
| Mean (pg/mL) | 45 | 70 | 167 | 50 | 84 | 189 |
| Standard Deviation | 2.5 | 3.2 | 8.5 | 3.8 | 8.2 | 12.9 |
| CV% | 5.6 | 4.6 | 5.1 | 7.6 | 9.8 | 6.8 |
Recovery for Mouse TSLP Quantikine ELISA Kit
The recovery of mouse TSLP spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=6) | 103 | 93-114 |
| EDTA Plasma (n=4) | 94 | 80-108 |
| Heparin Plasma (n=5) | 96 | 89-119 |
| Serum (n=5) | 92 | 87-101 |
Linearity
To assess the linearity of the assay, samples spiked with high concentrations of mouse TSLP in each matrix were diluted with Calibrator Diluent and then assayed.
Scientific Data Images for Mouse TSLP Quantikine ELISA Kit
Mouse TSLP ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: TSLP
Long Name
Thymic Stromal Lymphopoietin
Alternate Names
thymic stromal lymphopoietin
Gene Symbol
TSLP
Additional TSLP Products
Product Documents for Mouse TSLP Quantikine ELISA Kit
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse TSLP Quantikine ELISA Kit
For research use only
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.Citations for Mouse TSLP Quantikine ELISA Kit
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Sample Tested: Cell culture supernatantVerified Customer | Posted 02/26/2020Used Mouse TSLP ELISA for analysing organoid supernatants. Very easy to use kit, straight forward instructions and nice results. Standard curve came out very nicely. Would highly recommend.
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Protocols
View specific protocols for Mouse TSLP Quantikine ELISA Kit (MTLP00):
Refer to the product for complete assay procedure.
Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 50 µL of Assay Diluent to each well.
- Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
- Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.
- Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
- Aspirate and wash 5 times.
- Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
- Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
50 µL Assay Diluent
50 µL Standard, Control, or Sample
100 µL Conjugate
100 µL Substrate Solution
100 µL Stop Solution
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- Troubleshooting Guide: ELISA
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
