Mouse ZNRF3 Antibody Summary
Accession # Q5SSZ7
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
ZNRF3 in HEK Transfectants. ZNRF3 was detected in immersion fixed HEK human embryonic kidney cell transfectants using Rabbit Anti-Mouse ZNRF3 Monoclonal Antibody (Catalog # MAB8328) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
ZNRF3 (Zinc/RING Finger protein 3) is a member of the ZNRF3 family of type 1 transmembrane ubiquitin ligases (1). Mature mouse ZNRF3 consists of a 163 amino acid (aa) extracellular domain (ECD) with a protease associated (PA) domain fold, a short transmembrane segment, and a 675 aa cytoplasmic tail that contains a RING-type zinc finger with E3 ubiquitin ligase activity. Within the ECD, mouse and human ZNRF3 share 98% sequence identity. ZNRF3 is co-expressed on the cell surface with the homologous protein RNF43. Both proteins serve to inhibit the Wnt signaling pathway through the ubiquitination of LRP6 and a majority of the Frizzled family of Wnt receptors (1, 4, 6). ZNRF3 ubiquitin ligase activity is regulated jointly by R-Spondin and its cell surface receptors, Lgr4, 5, and 6 (2). The R-Spondin-Lgr complex binds to and facilitates the removal of the ZNRF3 from the plasma membrane, resulting in an enhancement of Wnt signaling (3, 4). Conversely, ZNRF3 can antagonize R-Spondin enhanced Wnt signaling (3, 5). ZNRF3 is highly expressed in crypt stem cells of the intestine where it modulates Wnt-induced cell proliferation to control the turnover of intestinal epithelial cells (6). ZNRF3 expression is down-regulated in gastric carcinomas, and ZNRF3 mutations are linked to carcinomas of the gastric tract, pancreas, liver, and ovary (7-10).
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- Peng, W.C. et al. (2013) PLoS One 8:e83110.
- Hao, H.X. et al. (2012) Nature 485:195.
- Moad, H.E. and A.A. Pioszak (2013) Biochemistry 52:7295.
- Koo, B.K. et al. (2012) Nature 488:665.
- Ong, C.K. et al. (2012) Nat. Genet. 44:690.
- Zhou, Y. et al. (2013) J. Mol. Histol. 44:555.
- Ryland, G.L. et al. (2013) J. Pathol. 229:469.
- Jiang, X. et al. (2013) Proc. Natl. Acad. Sci. USA 110:12649.
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