Mouse ZNRF3 Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB8328-100
MAB8328-SP
Mouse ZNRF3 Antibody in Data
1 Image
Product Details
FAQs
Supplemental Products
Reviews

Mouse ZNRF3 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse ZNRF3 in direct ELISAs.
Source
Recombinant Monoclonal Rabbit IgG Clone # 2035A
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse ZNRF3
Lys53-Met216
Accession # Q5SSZ7
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunocytochemistry
5-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunocytochemistry ZNRF3 antibody in HEK Transfectants by Immunocytochemistry (ICC). View Larger

ZNRF3 in HEK Transfectants. ZNRF3 was detected in immersion fixed HEK human embryonic kidney cell transfectants using Rabbit Anti-Mouse ZNRF3 Monoclonal Antibody (Catalog # MAB8328) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ZNRF3

ZNRF3 (Zinc/RING Finger protein 3) is a member of the ZNRF3 family of type 1 transmembrane ubiquitin ligases (1). Mature mouse ZNRF3 consists of a 163 amino acid (aa) extracellular domain (ECD) with a protease associated (PA) domain fold, a short transmembrane segment, and a 675 aa cytoplasmic tail that contains a RING-type zinc finger with E3 ubiquitin ligase activity. Within the ECD, mouse and human ZNRF3 share 98% sequence identity. ZNRF3 is co-expressed on the cell surface with the homologous protein RNF43. Both proteins serve to inhibit the Wnt signaling pathway through the ubiquitination of LRP6 and a majority of the Frizzled family of Wnt receptors (1, 4, 6). ZNRF3 ubiquitin ligase activity is regulated jointly by R-Spondin and its cell surface receptors, Lgr4, 5, and 6 (2). The R-Spondin-Lgr complex binds to and facilitates the removal of the ZNRF3 from the plasma membrane, resulting in an enhancement of Wnt signaling (3, 4). Conversely, ZNRF3 can antagonize R-Spondin enhanced Wnt signaling (3, 5). ZNRF3 is highly expressed in crypt stem cells of the intestine where it modulates Wnt-induced cell proliferation to control the turnover of intestinal epithelial cells (6). ZNRF3 expression is down-regulated in gastric carcinomas, and ZNRF3 mutations are linked to carcinomas of the gastric tract, pancreas, liver, and ovary (7-10).

References
  1. de Lau, W. et al. (2014) Genes Dev. 28:305.
  2. Xie, Y. et al. (2013) EMBO Rep. 14:1120.
  3. Peng, W.C. et al. (2013) PLoS One 8:e83110.
  4. Hao, H.X. et al. (2012) Nature 485:195.
  5. Moad, H.E. and A.A. Pioszak (2013) Biochemistry 52:7295.
  6. Koo, B.K. et al. (2012) Nature 488:665.
  7. Ong, C.K. et al. (2012) Nat. Genet. 44:690.
  8. Zhou, Y. et al. (2013) J. Mol. Histol. 44:555.
  9. Ryland, G.L. et al. (2013) J. Pathol. 229:469.
  10. Jiang, X. et al. (2013) Proc. Natl. Acad. Sci. USA 110:12649.
Long Name
Zinc and Ring Finger 3
Entrez Gene IDs
84133 (Human); 407821 (Mouse)
Alternate Names
BK747E2.3; KIAA1133; novel C3HC4 type Zinc finger (ring finger); RNF203; zinc and ring finger 3; zinc/RING finger protein 3; ZNRF3

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Cell and Tissue Staining Kits

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Mouse ZNRF3 Antibody

There are currently no reviews for this product. Be the first to review Mouse ZNRF3 Antibody and earn rewards!

Have you used Mouse ZNRF3 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review