MRPL21 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-13612

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the amino acids: ACSHSILRPSGPGAASLWSASRRFNSQSTSYLPGYVPKTSLSSPPWPEVVLPDPVEETRHHAEVV

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (83%), Rat (82%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MRPL21 Antibody - BSA Free

Western Blot: MRPL21 Antibody [NBP2-13612]

Western Blot: MRPL21 Antibody [NBP2-13612]

Western Blot: MRPL21 Antibody [NBP2-13612] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4
Immunocytochemistry/ Immunofluorescence: MRPL21 Antibody [NBP2-13612]

Immunocytochemistry/ Immunofluorescence: MRPL21 Antibody [NBP2-13612]

Immunocytochemistry/Immunofluorescence: MRPL21 Antibody [NBP2-13612] - Staining of human cell line CACO-2 shows localization to nucleoplasm & mitochondria.
Immunohistochemistry-Paraffin: MRPL21 Antibody [NBP2-13612]

Immunohistochemistry-Paraffin: MRPL21 Antibody [NBP2-13612]

Immunohistochemistry-Paraffin: MRPL21 Antibody [NBP2-13612] - Staining of human salivary gland shows granular cytoplasmic positivity in glandular cells.
MRPL21 Antibody - BSA Free Western Blot: MRPL21 Antibody - BSA Free [NBP2-13612]

Western Blot: MRPL21 Antibody - BSA Free [NBP2-13612]

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Human cell line RT-4

Applications for MRPL21 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MRPL21

Belonging to the ribosomal protein L21P family, MRPL21 plays an important role in translation and RNA binding. These Mammalian mitochondrial ribosomal proteins contain a small 28S subunit and a large 39S subunit, and consist of 75% protein to rRNA composition. The ribosomal proteins also help in protein synthesis within the mitochondrion. This gene also acts as a structural component of mitochondrial ribosomes, and encodes the 39S subunit protein.

Alternate Names

L21mt, MGC62013, mitochondrial ribosomal protein L21, MRP-L21,39S ribosomal protein L21, mitochondrial

Gene Symbol

MRPL21

Additional MRPL21 Products

Product Documents for MRPL21 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for MRPL21 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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