MRPL45 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-93711

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all MRPL45 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 57-306 of human MRPL45 (NP_115727.5). MQHARKAGLVIPPEKSDRSIHLACTAGIFDAYVPPEGDARISSLSKEGLIERTERMKKTMASQVSIRRIKDYDANFKIKDFPEKAKDIFIEAHLCLNNSDHDRLHTLVTEHCFPDMTWDIKYKTVRWSFVESLEPSHVVQVRCSSMMNQGNVYGQITVRMHTRQTLAIYDRFGRLMYGQEDVPKDVLEYVVFEKQLTNPYGSWRMHTKIVPPWAPPKQPILKTVMIPGPQLKPEEEYEEAQGEAQKPQLA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MRPL45 Antibody - Azide and BSA Free

Western Blot: MRPL45 AntibodyAzide and BSA Free [NBP2-93711]

Western Blot: MRPL45 AntibodyAzide and BSA Free [NBP2-93711]

Western Blot: MRPL45 Antibody [NBP2-93711] - Analysis of extracts of various cell lines, using MRPL45 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.
Immunohistochemistry-Paraffin: MRPL45 Antibody - Azide and BSA Free [NBP2-93711]

Immunohistochemistry-Paraffin: MRPL45 Antibody - Azide and BSA Free [NBP2-93711]

Immunohistochemistry-Paraffin: MRPL45 Antibody [NBP2-93711] - Paraffin-embedded human breast cancer using MRPL45.
Immunocytochemistry/ Immunofluorescence: MRPL45 Antibody - Azide and BSA Free [NBP2-93711]

Immunocytochemistry/ Immunofluorescence: MRPL45 Antibody - Azide and BSA Free [NBP2-93711]

Immunocytochemistry/Immunofluorescence: MRPL45 Antibody [NBP2-93711] - Immunofluorescence analysis of Hela cells using MRPL45 Rabbit pAb (NBP2-93711) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.

Applications for MRPL45 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MRPL45

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within themitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. Theyhave an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed.Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA.Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes inbiochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein.Pseudogenes corresponding to this gene are found on chromosomes 2p and 17q. (provided by RefSeq)

Alternate Names

L45mt, MGC11321, mitochondrial ribosomal protein L45, MRP-L45,39S ribosomal protein L45, mitochondrial

Gene Symbol

MRPL45

Additional MRPL45 Products

Product Documents for MRPL45 Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for MRPL45 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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Protocols

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