Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all MRPS5 Primary Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with E. coli-derived Human MRPS5 fragment.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for MRPS5 Antibody - BSA Free

Western Blot: MRPS5 Antibody [NBP2-98525]

Western Blot: MRPS5 Antibody [NBP2-98525]

Western Blot: MRPS5 Antibody [NBP2-98525] - Anti-MRPS5 rabbit polyclonal antibody at 1:500 dilution. Lane A: A431 Whole Cell Lysate Lane B: H1299 Whole Cell Lysate Lane C: HeLa Whole Cell Lysate Lane D: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 48 kDa. Observed band size: 45 kDa
Immunohistochemistry-Paraffin: MRPS5 Antibody [NBP2-98525]

Immunohistochemistry-Paraffin: MRPS5 Antibody [NBP2-98525]

Immunohistochemistry-Paraffin: MRPS5 Antibody [NBP2-98525] - Immunochemical staining of human MRPS5 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.
Immunohistochemistry-Paraffin: MRPS5 Antibody [NBP2-98525]

Immunohistochemistry-Paraffin: MRPS5 Antibody [NBP2-98525]

Immunohistochemistry-Paraffin: MRPS5 Antibody [NBP2-98525] - Immunochemical staining of human MRPS5 in human gallbladder with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.
Immunoprecipitation: MRPS5 Antibody [NBP2-98525]

Immunoprecipitation: MRPS5 Antibody [NBP2-98525]

Immunoprecipitation: MRPS5 Antibody [NBP2-98525] - MRPS5 was immunoprecipitated using: Lane A: 0.5 mg A431 Whole Cell Lysate 4 ul anti-MRPS5 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-MRPS5 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 48 kDa. Observed band size: 43 kDa
MRPS5 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: MRPS5 Antibody [NBP2-98525] -

Immunocytochemistry/Immunofluorescence: MRPS5 Antibody [NBP2-98525] - Immunofluorescence staining of MRPS5 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-98525 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.

Applications for MRPS5 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunohistochemistry

1:50-1:200

Immunohistochemistry-Paraffin

1:50-1:200

Immunoprecipitation

1-5uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Format

BSA Free

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MRPS5

MRPS5, or Mitochondrial Ribosomal Protein S5, contains a long 48 kDa isoform and a short 28 kDa isoform, and is involved in protein synthesis as a component of the 28S small mitochondrial ribosome subunit. Current research is being conducted on MRPS5 and its relation to several diseases and disorders, including mycobacterium tuberculosis, pneumonia, and tuberculosis. The protein interacts with MRPS2, ICT1, ESR2, DDX56, and AGO1, and has been associated with the process of translation.

Alternate Names

mitochondrial 28S ribosomal protein S5, mitochondrial ribosomal protein S5, MRP-S5S5mt28S ribosomal protein S5, mitochondrial

Gene Symbol

MRPS5

Additional MRPS5 Products

Product Documents for MRPS5 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for MRPS5 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for MRPS5 Antibody - BSA Free

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Protocols

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FAQs

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