MSH2 Antibody (1B3)

Name: MSH2 Antibody (1B3)
Brand: Novus Biologicals
SKU: NB100-1767
Price: 389 USD
Availability: InStock

Novus Biologicals | Catalog # NB100-1767

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Product Documents

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Monkey, Primate

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # 1B3

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Product Specifications

Immunogen

Purified recombinant fragment of human MSH2 expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Theoretical MW

105 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Mouse MSH2 Antibody (1B3) (NB100-1767) is a monoclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for MSH2 Antibody (1B3)

Western Blot: MSH2 Antibody (1B3)BSA Free [NB100-1767]

Western Blot: MSH2 Antibody (1B3) [NB100-1767] - Analysis using MSH2 mouse mAb against Hela (1), A549 (2), A431 (3) and HEK293 (4) cell lysate.

Immunocytochemistry/ Immunofluorescence: MSH2 Antibody (1B3) - BSA Free [NB100-1767]

Immunocytochemistry/Immunofluorescence: MSH2 Antibody (1B3) [NB100-1767] - Analysis of Hela cells using MSH2 mouse mAb (green), showing nuclear localization. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Immunohistochemistry-Paraffin: MSH2 Antibody (1B3) - BSA Free [NB100-1767]

Immunohistochemistry-Paraffin: MSH2 Antibody (1B3) [NB100-1767] - Analysis of human breast cancer (left) and lung cancer (right) tissues, showing nuclear localization using MSH2 mouse mAb with DAB staining.

Applications for MSH2 Antibody (1B3)

Application

Recommended Usage

ELISA

1:10000

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Ascites

Formulation

Ascites

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MSH2

MSH2 is a 100 kDa nuclear antigen and encodes a protein of 934 amino acids. The MSH2 gene is one of 4 known genes encoding proteins involved in the rapair of mismatch nucleotides following DNA replications or repair. Mutations in the NSH2 gene contribute to the development of sporadic colorectal carcinoma. MSHS mutations are responsible for 50% of inherited non-polyposis colorectal (HNPCC). The repair of mismatch DNA is essential to maintaining the integrity of genetic information over time. An alteration of microsatellite repeats is the result of slippage owing to strand misalignment during DNA replication and is referred to as microsatellite instability (MSI). These defects in DNA repair pathways have been related to human carcinogenesis. MSH-2 is involved in the intial cognition of mismatch nucleotides during the replication mismatch repair process.

Long Name

MutationS Homolog 2

Alternate Names

COCA1, FCC1, HNPCC1, LCFS2

Entrez Gene IDs

4436 (Human)

Gene Symbol

MSH2

Additional MSH2 Products

Product Documents for MSH2 Antibody (1B3)

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Product Specific Notices for MSH2 Antibody (1B3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers

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