MSH2 Antibody (CL13713) - BSA Free

Novus Biologicals | Catalog # NBP3-24572

Novus Biologicals

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (95%), Rat (90%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # CL13713

Format

BSA Free
View all MSH2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a synthetic peptide derived from P43246, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for MSH2 Antibody (CL13713) - BSA Free

Western Blot: MSH2 Antibody (CL13713) [NBP3-24572]

Western blot analysis in human cell line NTERA-2.

Immunohistochemistry-Paraffin: MSH2 Antibody (CL13713) [NBP3-24572]

Analysis in human testis and liver tissues using NBP3-24572 antibody. Corresponding MSH2 RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: MSH2 Antibody (CL13713) [NBP3-24572]

Staining of human lymph node shows strong nuclear positivity in germinal center cells.

Immunohistochemistry-Paraffin: MSH2 Antibody (CL13713) [NBP3-24572]

Staining of human testis cancer (seminoma) shows strong nuclear positivity in tumor cells.

Immunocytochemistry/Immunofluorescence: MSH2 Antibody (CL13713) [NBP3-24572]

Staining in NTERA-2 cell line with Anti-MSH2 monoclonal antibody, showing specific staining of nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).

Immunohistochemistry-Paraffin: MSH2 Antibody (CL13713) [NBP3-24572]

Staining of human colorectal cancer shows strong nuclear positivity in tumor cells.

Immunohistochemistry-Paraffin: MSH2 Antibody (CL13713) [NBP3-24572]

Staining of human ovarian cancer (high grade serous ovarian carcinoma) shows strong nuclear positivity in tumor cells.

Immunohistochemistry-Paraffin: MSH2 Antibody (CL13713) [NBP3-24572]

Staining of human breast cancer (ductal carcinoma) shows strong nuclear positivity in tumor cells.

Immunohistochemistry-Paraffin: MSH2 Antibody (CL13713) [NBP3-24572]

Staining of human liver shows no nuclear positivity in hepatocytes as expected.

Immunohistochemistry-Paraffin: MSH2 Antibody (CL13713) [NBP3-24572]

Staining of human small intestine shows strong nuclear positivity in glandular cells.

Immunohistochemistry-Paraffin: MSH2 Antibody (CL13713) [NBP3-24572]

Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.

Applications for MSH2 Antibody (CL13713) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

1ug/ml
Application Notes
For IHC-Paraffin, we recommend using Heat-Induced Epitope Retrieval (HIER) with a pH level of 6. This optimized retrieval method ensures the best results for your experiments, For ICC/IF, we recommend using a combination of PFA and Triton X-100. This will give you the optimal results for your experiments.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MSH2

MSH2 was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). When cloned, it was discovered to be a human homolog of the E. coli mismatch repair gene mutS, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC.

Long Name

MutationS Homolog 2

Alternate Names

COCA1, FCC1, HNPCC1, LCFS2

Gene Symbol

MSH2

Additional MSH2 Products

Product Documents for MSH2 Antibody (CL13713) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for MSH2 Antibody (CL13713) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers

Customer Reviews for MSH2 Antibody (CL13713) - BSA Free

There are currently no reviews for this product. Be the first to review MSH2 Antibody (CL13713) - BSA Free and earn rewards!

Have you used MSH2 Antibody (CL13713) - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies