MSX1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: MSX1 Antibody - BSA Free [NBP2-57969] -

Msx1+ SSCs subset was locally expanded by the in situ culture system with NSs.a Visualization of SSC2 differentiation lineage cells in osteo-lineage cells with UMAP plot, highlighting four specific subsets. b UMAP plot of the four specific subsets. c Relative proportion of cell subsets between Defect and NSs group. d Heatmap and the expression of marker genes of 8 osteo-lineage sub-clusters. Marker genes are provided in source data. e Barplot showing the GO enrichment in SSC2 subset. Markers used for enrichment analysis were selected according to p value (*p < 0.05, Wilcoxon Rank Sum test) and fold change (>1). Color bar represented the adjusted p values performed by R package clusterProfiler (Benjamini–Hochberg). f Violin plot showing the SSC2 top marker gene expression levels across the whole 8 different subsets. g Co-immunostaining of Msx1 and Mmp13 expression of paraffin sections in Untreated Defect group and Scaffold + NSs group at 2 weeks after defect surgery (bar = 200 μm at low magnification and bar = 30 μm at high magnification), at least three times of experiments were repeated independently. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36064711), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: MSX1 Antibody - BSA Free [NBP2-57969] -

In situ expansion of Msx1+ SSCs subset promoted efficient bone regeneration partially through endochondral ossification.a Trajectory of differentiation from cycling MSCs to both SSC1 and SSC2 lineages predicted by Monocle 2. b Heatmap of gene expressions in subsets ordered by pseudotime of two differentiation trajectories in a. c Distribution of cells on both the differentiation trajectories from Defect and NSs groups showing a featured change dominated at 1 week and 2 weeks after surgery, respectively. d Relative expression level of anabolic and metabolic genes (Acan, Col6a5, Mmp13) of cartilage and Msx1 gene along the whole pseudotime. e Expression of the above chondrocyte-specific genes (Acan, Col6a5, Mmp13) and SSC2 marker gene (Msx1) visualized on differentiation trajectory. f Co-immunostaining of Msx1 and Acan expression of paraffin sections in Scaffold + NSs group at 1 week and 2 weeks after defect surgery, respectively (bar = 200 μm at low magnification and bar=30μm at high magnification). g Co-immunostaining of Msx1 and Mmp13 expression of paraffin sections in Scaffold + NSs group at 1 week and 2 weeks after defect surgery, respectively (bar = 200 μm at low magnification and bar = 30 μm at high magnification). h, i Safranin-O staining of paraffin sections in Untreated Defect group and Scaffold + NSs group at 1 week and 2 weeks after defect surgery, respectively (bar = 500 μm at low magnification and bar=50μm at high magnification). At least three times of experiments were repeated independently. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36064711), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: MSX1 Antibody - BSA Free [NBP2-57969] -

Msx1+ SSCs subset is a source of osteogenesis and closely correlates with endochondral ossification.a Schematic of the assessment of Msx1+ SSCs subset in differentiation hierarchy by Prx1-lineage tracing mice. b, c HE staining of newly formed calvarium tissues in skull bone injury at 2 weeks and 4 weeks after surgery, respectively. d–g Coronal sections of the calvarium tissues from Prx1Cre; RosatdTomato mice co-immunostained with Msx1 and Runx2 (d); Msx1 and OCN (e); Msx1 and ACAN (f); Msx1 and COL2 (g); Scale bars: low magnification: 30 μm, high magnification: 10 μm. At least three times of experiments were repeated independently. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36064711), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: MSX1 Antibody - BSA Free [NBP2-57969] -

Msx1+ SSCs subset is a source of osteogenesis and closely correlates with endochondral ossification.a Schematic of the assessment of Msx1+ SSCs subset in differentiation hierarchy by Prx1-lineage tracing mice. b, c HE staining of newly formed calvarium tissues in skull bone injury at 2 weeks and 4 weeks after surgery, respectively. d–g Coronal sections of the calvarium tissues from Prx1Cre; RosatdTomato mice co-immunostained with Msx1 and Runx2 (d); Msx1 and OCN (e); Msx1 and ACAN (f); Msx1 and COL2 (g); Scale bars: low magnification: 30 μm, high magnification: 10 μm. At least three times of experiments were repeated independently. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36064711), licensed under a CC-BY license. Not internally tested by Novus Biologicals.