MTAP Antibody

Novus Biologicals | Catalog # NBP1-32718

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all MTAP Primary Antibodies »

Product Specifications

Immunogen

Full length human MTAP Recombinant protein.

Reactivity Notes

Xenopus laevis (80%), Chicken (83%).

Localization

Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

31 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MTAP Antibody

Western Blot: MTAP Antibody [NBP1-32718]

Western Blot: MTAP Antibody [NBP1-32718]

Western Blot: MTAP Antibody [NBP1-32718] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with MTAP antibody diluted at a dilution of 1:5000.
Immunocytochemistry/ Immunofluorescence: MTAP Antibody [NBP1-32718]

Immunocytochemistry/ Immunofluorescence: MTAP Antibody [NBP1-32718]

Immunocytochemistry/Immunofluorescence: MTAP Antibody [NBP1-32718] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MTAP protein stained by MTAP antibody [N1C3] diluted at 1:500. Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: MTAP Antibody [NBP1-32718]

Immunohistochemistry-Paraffin: MTAP Antibody [NBP1-32718]

Immunohistochemistry-Paraffin: MTAP Antibody [NBP1-32718] - SAS xenograft, using MTAP antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Western Blot: MTAP Antibody [NBP1-32718]

Western Blot: MTAP Antibody [NBP1-32718]

Western Blot: MTAP Antibody [NBP1-32718] - Sample (50 ug of whole cell lysate) A: mouse liver 12% SDS PAGE; antibody diluted at 1:1000.
Immunoprecipitation: MTAP Antibody [NBP1-32718]

Immunoprecipitation: MTAP Antibody [NBP1-32718]

Immunoprecipitation: MTAP Antibody [NBP1-32718] - Immunoprecipitation of MTAP protein from HeLa whole cell extracts using 5 ug of MTAP antibody [N1C3]. Western blot analysis was performed using MTAP antibody [N1C3]. EasyBlot anti-Rabbit IgG was used as a secondary reagent.

Applications for MTAP Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MTAP

This gene encodes an enzyme that plays a major role in polyamine metabolism and is important for the salvage of both adenine and methionine. The encoded enzyme is deficient in many cancers because this gene and the tumor suppressor p16 gene are co-deleted. Multiple alternatively spliced transcript variants have been described for this gene, but their full-length natures remain unknown. [provided by RefSeq]

Long Name

5'-Methylthioadenosine Phosphorylase

Alternate Names

BDMF, C86fus, DMSFH, DMSMFH, HEL-249, LGMBF, MTAPase

Gene Symbol

MTAP

Additional MTAP Products

Product Documents for MTAP Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for MTAP Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Related Research Areas

Sulfation Regulators

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Protocols

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FAQs

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