MTHFS Antibody

Novus Biologicals | Catalog # NBP1-20947

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all MTHFS Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence KRCLQHQEVKPYT corresponding to internal region according to NP_006432.1.

Epitope

internal region

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for MTHFS Antibody

Western Blot: MTHFS Antibody [NBP1-20947]

Western Blot: MTHFS Antibody [NBP1-20947]

Western Blot: MTHFS Antibody [NBP1-20947] - (0.5ug/ml) Analysis of Human Liver lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: MTHFS Antibody [NBP1-20947]

Immunohistochemistry-Paraffin: MTHFS Antibody [NBP1-20947]

Immunohistochemistry-Paraffin: MTHFS Antibody [NBP1-20947] - Staining of paraffin embedded Human Lung at dilution of 5ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for MTHFS Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

5 ug/ml

Peptide ELISA

Detection limit 1:32000

Western Blot

0.5 - 1.5 ug/ml
Application Notes
WB: Approx. 25 kDa band observed in human liver lysates (calculated MW of 23.3 kDa band according to NP_006432.1).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MTHFS

Contributes to tetrahydrofolate metabolism. Helps regulate carbon flow through the folate-dependent one-carbon metabolic network that supplies carbon for the biosynthesis of purines, thymidine and amino acids

Alternate Names

5,10-methenyltetrahydrofolate synthetase (5-formyltetrahydrofolatecyclo-ligase), 5-formyltetrahydrofolate cyclo-ligase, EC 6.3.3.25,10-methenyl-tetrahydrofolate synthetase, FLJ30410, HsT19268, Methenyl-THF synthetase

Entrez Gene IDs

10588 (Human); 107885 (Mouse)

Gene Symbol

MTHFS

UniProt

NP_006432.1 (Human)

Additional MTHFS Products

Product Documents for MTHFS Antibody

Certificate of Analysis

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Product Specific Notices for MTHFS Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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