mtTFA Antibody - BSA Free

Western Blot: mtTFA Antibody [NBP2-19437] -

Western Blot: mtTFA Antibody [NBP2-19437] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with mtTFA antibody (NBP2-19437) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: mtTFA Antibody [NBP2-19437] -

Western Blot: mtTFA Antibody [NBP2-19437] - Non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with mtTFA antibody diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry-Paraffin: mtTFA Antibody [NBP2-19437] -

mtTFA antibody detects mtTFA protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
mtTFA stained by mtTFA antibody (NBP2-19437) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunocytochemistry/ Immunofluorescence: mtTFA Antibody [NBP2-19437] -

mtTFA antibody detects mtTFA protein at mito by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: mtTFA stained by mtTFA antibody (NBP2-19437) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.
Blue: Fluoroshield with DAPI.

Immunoprecipitation: mtTFA Antibody [NBP2-19437] -

mtTFA antibody immunoprecipitates mtTFA protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. 40 ug 293T whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of mtTFA protein by 2 ug of mtTFA antibody (NBP2-19437) 15% SDS-PAGE The immunoprecipitated mtTFA protein was detected by mtTFA antibody (NBP2-19437) diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.