mtTFA Antibody

Novus Biologicals | Catalog # NBP2-19438

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Chimpanzee (99%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all mtTFA Primary Antibodies »

Product Specifications

Immunogen

Full length human mtTFA Recombinant protein.

Localization

Mitochondrion

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for mtTFA Antibody

Immunohistochemistry-Paraffin: mtTFA Antibody [NBP2-19438]

Immunohistochemistry-Paraffin: mtTFA Antibody [NBP2-19438]

Immunohistochemistry-Paraffin: mtTFA Antibody [NBP2-19438] - Human breast cancer, using mtTFA antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Western Blot: mtTFA Antibody [NBP2-19438]

Western Blot: mtTFA Antibody [NBP2-19438]

Western Blot: mtTFA Antibody [NBP2-19438] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with mtTFA antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
mtTFA Antibody

Western Blot: mtTFA Antibody [NBP2-19438] -

Western Blot: mtTFA Antibody [NBP2-19438] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with mtTFA antibody (NBP2-19438) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
mtTFA Antibody

Immunohistochemistry-Paraffin: mtTFA Antibody [NBP2-19438] -

Immunohistochemistry-Paraffin: mtTFA Antibody [NBP2-19438] - mtTFA antibody detects mtTFA protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded human esophageal carcinoma.
mtTFA stained by mtTFA antibody (NBP2-19438) diluted at 1:165.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
mtTFA Antibody

Immunocytochemistry/ Immunofluorescence: mtTFA Antibody [NBP2-19438] -

TFAM antibody detects TFAM protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TFAM stained by TFAM antibody (NBP2-19438) diluted at 1:1000.
mtTFA Antibody

Western Blot: mtTFA Antibody [NBP2-19438] -

Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with mtTFA antibody (NBP2-19438) diluted at 1:1000.
mtTFA Antibody

Immunocytochemistry/ Immunofluorescence: mtTFA Antibody [NBP2-19438] -

mtTFA antibody detects mtTFA protein at mitochondria by immunofluorescent analysis.
Sample: NT2D1 cells were fixed in 2% paraformaldehyde/culture medium at RT for 30 min.
Green: mtTFA protein stained by mtTFA antibody (NBP2-19438) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 um.
mtTFA Antibody

Western Blot: mtTFA Antibody [NBP2-19438] -

Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with mtTFA antibody (NBP2-19438) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
mtTFA Antibody

Immunocytochemistry/ Immunofluorescence: mtTFA Antibody [NBP2-19438] -

mtTFA antibody detects mtTFA protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: mtTFA protein stained by mtTFA antibody (NBP2-19438) diluted at 1:500.
Red: Mitotracker, a mitochondria marker, diluted at 1:2000.
Blue: Hoechst 33342 staining.
mtTFA Antibody

Immunoprecipitation: mtTFA Antibody [NBP2-19438] -

mtTFA antibody immunoprecipitates mtTFA protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. 40 ug 293T whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of mtTFA protein by 2 ug of mtTFA antibody (NBP2-19438) 12% SDS-PAGE The immunoprecipitated mtTFA protein was detected by mtTFA antibody (NBP2-19438) diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.

Applications for mtTFA Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: mtTFA

This gene encodes a mitochondrial transcription factor that is a key activator of mitochondrial transcription as well as a participant in mitochondrial genome replication. Studies in mice have demonstrated that this gene product is required to regulate the mitochondrial genome copy number and is essential for embryonic development. A mouse model for Kearns-Sayre syndrome was produced when expression of this gene was eliminated by targeted disruption in heart and muscle cells. [provided by RefSeq]

Alternate Names

mitochondrial transcription factor A, MTTF1, mtTFA, TCF-6, TCF6L1, TCF6L2Mitochondrial transcription factor 1, TCF6TCF6L3, Transcription factor 6, Transcription factor 6-like 2, Transcription factor 6-like 2 (mitochondrial transcription factor), transcription factor A, mitochondrial

Gene Symbol

TFAM

UniProt

Q00059 (Human)

Additional mtTFA Products

Product Documents for mtTFA Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for mtTFA Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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FAQs

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