mu Crystallin Antibody (OTI1G7)

Novus Biologicals | Catalog # NBP2-02829

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI1G7
View all mu Crystallin Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human CRYM (NP_001879) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

33.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for mu Crystallin Antibody (OTI1G7)

Western Blot: mu Crystallin Antibody (OTI1G7) [NBP2-02829]

Western Blot: mu Crystallin Antibody (OTI1G7) [NBP2-02829]

Western Blot: mu Crystallin Antibody (OTI1G7) [NBP2-02829] - analysis of extracts (10ug) from 10 Human tissue by using anti-CRYM monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
Immunocytochemistry/ Immunofluorescence: mu Crystallin Antibody (OTI1G7) [NBP2-02829]

Immunocytochemistry/ Immunofluorescence: mu Crystallin Antibody (OTI1G7) [NBP2-02829]

Immunocytochemistry/Immunofluorescence: mu Crystallin Antibody (OTI1G7) [NBP2-02829] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY mu Crystallin.
Immunohistochemistry-Paraffin: mu Crystallin Antibody (OTI1G7) [NBP2-02829]

Immunohistochemistry-Paraffin: mu Crystallin Antibody (OTI1G7) [NBP2-02829]

Immunohistochemistry-Paraffin: mu Crystallin Antibody (1G7) [NBP2-02829] - Staining of paraffin-embedded Human prostate tissue using anti-mu Crystallin mouse monoclonal antibody.
Flow Cytometry: mu Crystallin Antibody (OTI1G7) [NBP2-02829]

Flow Cytometry: mu Crystallin Antibody (OTI1G7) [NBP2-02829]

Flow Cytometry: mu Crystallin Antibody (OTI1G7) [NBP2-02829] - HEK293T cells transfected with either RC218123 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained and then analyzed by flow cytometry.
Western Blot: mu Crystallin Antibody (1G7) [NBP2-02829] Analysis of extracts (35ug) from 9 different cell lines by using anti-mu Crystallin monoclonal antibody.
Western Blot: mu Crystallin Antibody (OTI1G7) [NBP2-02829]

Western Blot: mu Crystallin Antibody (OTI1G7) [NBP2-02829]

Western Blot: mu Crystallin Antibody (1G7) [NBP2-02829] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY mu Crystallin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-mu Crystallin.
Western Blot: mu Crystallin Antibody (OTI1G7) [NBP2-02829]

Western Blot: mu Crystallin Antibody (OTI1G7) [NBP2-02829]

Western Blot: mu Crystallin Antibody (OTI1G7) [NBP2-02829] - analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-CRYM monoclonal antibody (1:200).
Immunohistochemistry-Paraffin: mu Crystallin Antibody (OTI1G7) [NBP2-02829]

Immunohistochemistry-Paraffin: mu Crystallin Antibody (OTI1G7) [NBP2-02829]

Immunohistochemistry-Paraffin: mu Crystallin Antibody (1G7) [NBP2-02829] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-mu Crystallin mouse monoclonal antibody.
Immunohistochemistry-Paraffin: mu Crystallin Antibody (OTI1G7) [NBP2-02829]

Immunohistochemistry-Paraffin: mu Crystallin Antibody (OTI1G7) [NBP2-02829]

Immunohistochemistry-Paraffin: mu Crystallin Antibody (1G7) [NBP2-02829] - Staining of paraffin-embedded Human breast tissue using anti-mu Crystallin mouse monoclonal antibody.
Immunohistochemistry-Paraffin: mu Crystallin Antibody (OTI1G7) [NBP2-02829]

Immunohistochemistry-Paraffin: mu Crystallin Antibody (OTI1G7) [NBP2-02829]

Immunohistochemistry-Paraffin: mu Crystallin Antibody (1G7) [NBP2-02829] - Staining of paraffin-embedded Human colon tissue using anti-mu Crystallin mouse monoclonal antibody.
Immunohistochemistry-Paraffin: mu Crystallin Antibody (OTI1G7) [NBP2-02829]

Immunohistochemistry-Paraffin: mu Crystallin Antibody (OTI1G7) [NBP2-02829]

Immunohistochemistry-Paraffin: mu Crystallin Antibody (1G7) [NBP2-02829] - Staining of paraffin-embedded Human Kidney tissue using anti-mu Crystallin mouse monoclonal antibody.
Immunohistochemistry-Paraffin: mu Crystallin Antibody (OTI1G7) [NBP2-02829]

Immunohistochemistry-Paraffin: mu Crystallin Antibody (OTI1G7) [NBP2-02829]

Immunohistochemistry-Paraffin: mu Crystallin Antibody (1G7) [NBP2-02829] - Staining of paraffin-embedded Human liver tissue using anti-mu Crystallin mouse monoclonal antibody.

Applications for mu Crystallin Antibody (OTI1G7)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:500-2000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: mu Crystallin

Crystallins are separated into two classes: taxon-specific and ubiquitous. The former class is also called phylogenetically-restricted crystallins. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. This gene encodes a taxon-specific crystallin protein that binds NADPH and has sequence similarity to bacterial ornithine cyclodeaminases. The encoded protein does not perform a structural role in lens tissue, and instead it binds thyroid hormone for possible regulatory or developmental roles. Mutations in this gene have been associated with autosomal dominant non-syndromic deafness. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq].

Alternate Names

crystallin, mu, DFNA40NADP-regulated thyroid-hormone binding protein, mu-crystallin homolog, NADP-regulated thyroid-hormone-binding protein, THBP

Entrez Gene IDs

1428 (Human)

Gene Symbol

CRYM

Additional mu Crystallin Products

Product Documents for mu Crystallin Antibody (OTI1G7)

Certificate of Analysis

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Product Specific Notices for mu Crystallin Antibody (OTI1G7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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