MUC2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-31231

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation, Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence, Dot Blot

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence, Cytometric Bead Assay Standard, Dot Blot, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all MUC2 Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human MUC2. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (86%).

Localization

Secreted

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

540 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MUC2 Antibody - BSA Free

Immunohistochemistry: MUC2 Antibody [NBP1-31231]

Immunohistochemistry: MUC2 Antibody [NBP1-31231]

MUC2-Antibody-Immunohistochemistry-NBP1-31231-img0012.jpg
Immunocytochemistry/ Immunofluorescence: MUC2 Antibody [NBP1-31231]

Immunocytochemistry/ Immunofluorescence: MUC2 Antibody [NBP1-31231]

MUC2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31231-img0010.jpg
MUC2 Antibody

Immunohistochemistry-Paraffin: MUC2 Antibody [NBP1-31231] -

Immunohistochemistry-Paraffin: MUC2 Antibody [NBP1-31231] -Human intestine.MUC2 antibody [C3], C-term diluted at 1:750.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
MUC2 Antibody - BSA Free

Immunohistochemistry-Paraffin: MUC2 Antibody [NBP1-31231] -

MUC2 antibody [C3], C-term detects MUC2 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
MUC2 stained by MUC2 antibody [C3], C-term (NBP1-31231) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
MUC2 Antibody - BSA Free

Immunohistochemistry-Paraffin: MUC2 Antibody [NBP1-31231] -

MUC2 antibody [C3], C-term detects MUC2 protein by immunohistochemical analysis.
Sample: Paraffin-embedded human tissues.
MUC2 stained by MUC2 antibody [C3], C-term (NBP1-31231) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Corresponding RNA levels (RPKM) in the tissues are based on NCBI database.
MUC2 Antibody - BSA Free

Immunohistochemistry-Paraffin: MUC2 Antibody [NBP1-31231] -

MUC2 antibody [C3], C-term detects MUC2 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
MUC2 stained by MUC2 antibody [C3], C-term (NBP1-31231) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
MUC2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: MUC2 Antibody - BSA Free [NBP1-31231] -

Effect of SPI on mucin production in mouse ileum tissue. (A) qRT-PCR analysis of Muc1, Muc2, Tff3, Grp94, and Agr2 mRNA expression levels in mouse ileum tissue (normalized against Actb ( beta -actin); n = 8). (B,C) Representative PAS-stained histomicrographs and corresponding quantification of goblets cell per villus in the ileum tissue (n = 6). (D,E) Representative micrographs for MUC2 staining and corresponding quantification of MUC2+ goblet cells per villus in the ileum tissue (n = 6). All data are presented as means +/- SEM. * p < 0.05; ** p < 0.01. Statistical significance was calculated using t-tests. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32911830), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for MUC2 Antibody - BSA Free

Application
Recommended Usage

Dot Blot

Reported in scientific literature (PMID:32223302).

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Frozen

Assay dependent

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MUC2

MUC2, also called mucin 2, is a member of the mucin protein family which are heavily glycosylated, high molecular weight proteins that are produced by goblet cells in epithelial tissues including the intestinal and respiratory tract (1). Mucins can form gels and function in lubrication of mucosal surfaces which serve as a protective barrier of the epithelial lining and an innate host defense (1). Mucins are divided into two groups based on whether they are membrane-bound or secreted, where MUC2 belongs to the secretory group (1,2). Secreted mucins have a distinctive composition characterized by a large central exon "mucin domain" containing O-glycosylated tandem repeat regions rich in proline, threonine, and serine residues, non-repetitive domains, and cysteine-rich domains (1,2). The mucin domain is flanked by 5' and 3' D domains, a B-like domain, a C-like domain, and a Ck-domain (1,2). MUC2 was the first secretory mucin identified in humans (3,4). Full-length MUC2 protein is synthesized as 5179 amino acids (aa) in length with a theoretical molecular weight of ~540 kDa (5).

Changes or perturbations to MUC2 expression has been associated with a number of disease pathologies (1-4, 6). Specifically, altered MUC2 composition has been indicated in colorectal cancer, inflammatory bowel diseases (IBD) including ulcerative colitis and Chron's disease, and chronic obstructive pulmonary disease (COPD) (1-4, 6). In general, decreased or reduced MUC2 expression is associated with colorectal cancer disease progression and development of IBD (1-4, 6). Additionally, studies have found that upon intestinal infection due to parasites or bacteria MUC2 expression is increased, as is overall mucus secretion (3, 6).

References

1. Pothuraju, R., Krishn, S. R., Gautam, S. K., Pai, P., Ganguly, K., Chaudhary, S., Rachagani, S., Kaur, S., & Batra, S. K. (2020). Mechanistic and Functional Shades of Mucins and Associated Glycans in Colon Cancer. Cancers. https://doi.org/10.3390/cancers12030649

2. Ballester, B., Milara, J., & Cortijo, J. (2019). Mucins as a New Frontier in Pulmonary Fibrosis. Journal of Clinical Medicine. https://doi.org/10.3390/jcm8091447

3. Liu, Y., Yu, X., Zhao, J., Zhang, H., Zhai, Q., & Chen, W. (2020). The role of MUC2 mucin in intestinal homeostasis and the impact of dietary components on MUC2 expression. International Journal of Biological Macromolecules. https://doi.org/10.1016/j.ijbiomac.2020.07.191

4. Allen, A., Hutton, D. A., & Pearson, J. P. (1998). The MUC2 gene product: a human intestinal mucin. The International Journal of Biochemistry & Cell Biology. https://doi.org/10.1016/s1357-2725(98)00028-4

5. Uniprot (Q02817)

6. Kim, Y. S., & Ho, S. B. (2010). Intestinal goblet cells and mucins in health and disease: recent insights and progress. Current Gastroenterology Reports. https://doi.org/10.1007/s11894-010-0131-2

Alternate Names

Intestinal mucin-2, MLP, MUC2, MUC-2, mucin 2, intestinal/tracheal, mucin 2, oligomeric mucus/gel-forming, mucin-2, SMUC

Gene Symbol

MUC2

Additional MUC2 Products

Product Documents for MUC2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for MUC2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for MUC2 Antibody - BSA Free

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Protocols

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