MuRF1/TRIM63 Antibody (7B8E1) - BSA Free

Novus Biologicals | Catalog # NBP2-52516

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 7B8E1

Format

BSA Free
View all MuRF1/TRIM63 Primary Antibodies »

Product Specifications

Immunogen

Synthesized peptide of human MuRF1/TRIM63 (AA: 293-304).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

40.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MuRF1/TRIM63 Antibody (7B8E1) - BSA Free

Immunocytochemistry/ Immunofluorescence: MuRF1/TRIM63 Antibody (7B8E1) - BSA Free [NBP2-52516]

Immunocytochemistry/ Immunofluorescence: MuRF1/TRIM63 Antibody (7B8E1) - BSA Free [NBP2-52516]

Immunocytochemistry/Immunofluorescence: MuRF1/TRIM63 Antibody (7B8E1) [NBP2-52516] - Analysis of Hela cells using MuRF1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Immunohistochemistry: MuRF1/TRIM63 Antibody (7B8E1) - BSA Free [NBP2-52516]

Immunohistochemistry: MuRF1/TRIM63 Antibody (7B8E1) - BSA Free [NBP2-52516]

Immunohistochemistry: MuRF1/TRIM63 Antibody (7B8E1) [NBP2-52516] - Analysis of paraffin-embedded rectum cancer tissues using MuRF1 mouse mAb with DAB staining.
Flow Cytometry: MuRF1/TRIM63 Antibody (7B8E1) - BSA Free [NBP2-52516]

Flow Cytometry: MuRF1/TRIM63 Antibody (7B8E1) - BSA Free [NBP2-52516]

Flow Cytometry: MuRF1/TRIM63 Antibody (7B8E1) [NBP2-52516] - Analysis of Hela cells using MuRF1 mouse mAb (green) and negative control (red).
Immunohistochemistry-Paraffin: MuRF1/TRIM63 Antibody (7B8E1) - BSA Free [NBP2-52516]

Immunohistochemistry-Paraffin: MuRF1/TRIM63 Antibody (7B8E1) - BSA Free [NBP2-52516]

Immunohistochemistry-Paraffin: MuRF1/TRIM63 Antibody (7B8E1) [NBP2-52516] - Analysis of paraffin-embedded muscle tissues using MuRF1 mouse mAb with DAB staining.
ELISA: MuRF1/TRIM63 Antibody (7B8E1) - BSA Free [NBP2-52516]

ELISA: MuRF1/TRIM63 Antibody (7B8E1) - BSA Free [NBP2-52516]

ELISA: MuRF1/TRIM63 Antibody (7B8E1) [NBP2-52516] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).

Applications for MuRF1/TRIM63 Antibody (7B8E1) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Immunohistochemistry

1:200-1:1000

Immunohistochemistry-Paraffin

1:200-1:1000
Application Notes
This antibody is Cytof ready.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MuRF1/TRIM63

MURF1 encodes a member of the RING zinc finger protein family found in striated muscle and iris. The product of this gene is localized to the Z-line and M-line lattices of myofibrils, where titin's N-terminal and C-terminal regions respectively bind to the sarcomere. In vitro binding studies have shown that this protein also binds directly to titin near the region of titin containing kinase activity. Another member of this protein family binds to microtubules. Since these family members can form heterodimers, this suggests that these proteins may serve as a link between titin kinase and microtubule-dependent signal pathways in muscle.

Long Name

Tripartite Motif-containing 63

Alternate Names

IRF, RNF28, SMRZ, TRIM63

Gene Symbol

TRIM63

Additional MuRF1/TRIM63 Products

Product Documents for MuRF1/TRIM63 Antibody (7B8E1) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for MuRF1/TRIM63 Antibody (7B8E1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

E3 Ubiquitin Ligases

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Protocols

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FAQs

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Associated Pathways

Ubiquitination Cascade Pathway Ubiquitination Cascade Pathway Thumbnail