Q: What is the buffer composition of this antibody?

A: The MURF1 antibody (NBP2-11061) is purified on immobilized antigen based affinity chromatography. The affinity purified antibodies are stabilized in Tris/glycine buffer at pH 7.4 containing 0.02% sodium azide as preservative, cryo-protective agents, stabilizing proteins (BSA) and glycerol. The only cryo-protective agent used in the buffer of NBP2-11061 is glycerol (35%). According to the supplier of this antibody, no DMSO was used in the actual production of the antibody, it was only used at later stages (conjugation) at 10%.

MuRF1/TRIM63 Antibody

Novus Biologicals | Catalog # NBP2-11061

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot, ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

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Product Specifications

Immunogen

Synthetic peptide taken from human MURF1 protein taken from within the C-terminal domain of MURF1 corresponding to amino acid sequence. The peptide was conserved in several other species. The selected peptide was post-synthetically modified to achieve highest antigenicity before coupling to carrier protein using hetero bifunctional cross linker for immunogen preparation

Epitope

tlfltakqliksiveaskgc

Reactivity Notes

Rat, Mouse and Human

Specificity

Q969Q1 The antibody labels a strong diffuse band of MURF1of 41 kDa in PC-MURF1samples and in several muscle cell lines. The antibody also labels a diffuse band of 73kDa. The identity of this band is not known.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit MuRF1/TRIM63 Antibody (NBP2-11061) is a polyclonal antibody validated for use in IHC, WB, ELISA and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for MuRF1/TRIM63 Antibody

Western Blot: MuRF1/TRIM63 Antibody [NBP2-11061]

Western Blot: MURF1 Antibody [NBP2-11061] - WB of antibody with sample. Antibody dilution 1:500 in Dilution Buffer. MW of MURF1 is 41 and 52 kDa.

Applications for MuRF1/TRIM63 Antibody

Application

Recommended Usage

ELISA

1:500

Western Blot

1:500

Application Notes

MURF1antibodies are fully characterized for applications in western blotting and ELISA at the recommended dilutions.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris/Glycine buffer, pH 7.4-7.8, HEPES,BSA 0.5%, glycerol 30%.

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MuRF1/TRIM63

RING domain in proteins is a zinc finger motif which serves as a protein-protein interaction interface. The muscle specific RING finger protein (MURF-1) that binds specifically to tintin repeats A168/A169 adjecent to the tintin kinase domain of the giant myofibrillar protein (1). There are 3 genes encoding three different type of muscle specific RING finger proteins (MURF1-3). Muscles adapts to decrease loads and activity by undergoing atrophy. Though in rat atrophy model majority of the genes are up-regulated, but interestingly, two sets of genes (MURF1 and MAFbx) both encode for E3 ubiquitin ligases. Knockout mice for MURF1 and MAFbx (Atrogin) exhibit resistance to muscle atrophy in quadriceps muscle after lesion of its innervation (2). Both MURF1 and MAFbx are potential therapeutic targets for muscle atrophy. E3 ubiquitin ligase. regulates proteasomal degradation of cardiac troponin I/TNNI3 and probably of other sarcomeric-associated proteins. E3 ubiquitin ligase can be classified into two types based on their mechanism of transferring ubiquitin to the substrate. One type, which comprises most E3s, contains a Ring motif (or the related U-box motif) and facilitates transfer of the activated ubiquitin directly from the E2 to the substrate. The second type contains a HECT domain, which forms a thioester intermediate with ubiquitin and then transfers the ubiquitin to the substrate (3). Eukaryotic cells contain a large number of different E3s (probably hundreds), and each E3 is believed to recognize only a limited number of proteins as substrates. In most cases the E3 recognizes a specific structure or amino acid sequence in the substrate (often termed the "degron") (3). For example, the "N-end rule" E3, Ubr-1, recognizes particular types of amino acids at the N terminus of the substrates (4), whereas the E3 SCFCdc4 complex recognizes a specific phosphorylated amino acid sequence in its substrate, Sic1 (4). MURF- selective antibodies were generated against peptides form unique regions on MURF1 protein corresponding to amino acid 274-293 near the C-terminal end of protein. This region was not only antigenic but also lies on the surface of the protein in native or in partially denatured MURF1 confirmation

Long Name

Tripartite Motif-containing 63

Alternate Names

IRF, RNF28, SMRZ, TRIM63

Gene Symbol

TRIM63

Additional MuRF1/TRIM63 Products

Product Documents for MuRF1/TRIM63 Antibody

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Certificate of Analysis

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Product Specific Notices for MuRF1/TRIM63 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

E3 Ubiquitin Ligases

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