Musashi-2 Antibody (OTI2F10)

Novus Biologicals | Catalog # NBP2-45837

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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2F10
View all Musashi-2 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human MSI2(NP_620412) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Musashi-2 Antibody (OTI2F10)

Western Blot: Musashi-2 Antibody (OTI2F10) [NBP2-45837]

Western Blot: Musashi-2 Antibody (OTI2F10) [NBP2-45837]

Western Blot: Musashi-2 Antibody (OTI2F10) [NBP2-45837] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MSI2.
Immunohistochemistry: Musashi-2 Antibody (OTI2F10) [NBP2-45837]

Immunohistochemistry: Musashi-2 Antibody (OTI2F10) [NBP2-45837]

Immunohistochemistry: Musashi-2 Antibody (OTI2F10) [NBP2-45837] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Western Blot: Musashi-2 Antibody (OTI2F10) [NBP2-45837]

Western Blot: Musashi-2 Antibody (OTI2F10) [NBP2-45837]

Western Blot: Musashi-2 Antibody (OTI2F10) [NBP2-45837] - Analysis of extracts (35ug) from 9 different cell lines by using MSI2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200)
Immunohistochemistry: Musashi-2 Antibody (OTI2F10) [NBP2-45837]

Immunohistochemistry: Musashi-2 Antibody (OTI2F10) [NBP2-45837]

Immunohistochemistry: Musashi-2 Antibody (OTI2F10) [NBP2-45837] - Analysis of Adenocarcinoma of Human ovary tissu. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: Musashi-2 Antibody (OTI2F10) [NBP2-45837]

Immunohistochemistry: Musashi-2 Antibody (OTI2F10) [NBP2-45837]

Immunohistochemistry: Musashi-2 Antibody (OTI2F10) [NBP2-45837] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: Musashi-2 Antibody (OTI2F10) [NBP2-45837]

Immunohistochemistry: Musashi-2 Antibody (OTI2F10) [NBP2-45837]

Immunohistochemistry: Musashi-2 Antibody (OTI2F10) [NBP2-45837] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: Musashi-2 Antibody (OTI2F10) [NBP2-45837]

Immunohistochemistry: Musashi-2 Antibody (OTI2F10) [NBP2-45837]

Immunohistochemistry: Musashi-2 Antibody (OTI2F10) [NBP2-45837] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunofluorescence: Musashi-2 Antibody (OTI2F10) [NBP2-45837]

Immunofluorescence: Musashi-2 Antibody (OTI2F10) [NBP2-45837]

Immunofluorescence: Musashi-2 Antibody (OTI2F10) [NBP2-45837] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY Musashi-2.
Immunofluorescence: Musashi-2 Antibody (OTI2F10) [NBP2-45837]

Immunofluorescence: Musashi-2 Antibody (OTI2F10) [NBP2-45837]

Immunofluorescence: Musashi-2 Antibody (OTI2F10) [NBP2-45837] - Analysis of HeLa cells.

Applications for Musashi-2 Antibody (OTI2F10)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Western Blot

1:200 - 1:1000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Musashi-2

Musashi 2 encodes a protein containing two conserved tandem RNA recognition motifs. Similar proteins in other species function as RNA-binding proteins and play central roles in posttranscriptional gene regulation. Two transcript variants encoding distinct isoforms have been identified for this gene.

Alternate Names

MSI2, Musashi2

Gene Symbol

MSI2

Additional Musashi-2 Products

Product Documents for Musashi-2 Antibody (OTI2F10)

Certificate of Analysis

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Product Specific Notices for Musashi-2 Antibody (OTI2F10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Stem Cell Markers

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Protocols

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