MxA/Mx1 Antibody (HL2051) - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-25572
Recombinant Monoclonal Antibody
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Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Human, Mouse, Canine, Feline
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL2051 expressed in HEK293
Format
Azide and BSA Free
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Product Specifications
Immunogen
Recombinant fragment of human MxA/Mx1
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
76 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit MxA/Mx1 Antibody (HL2051) - Azide and BSA Free (NBP3-25572) is a recombinant monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for MxA/Mx1 Antibody (HL2051) - Azide and BSA Free
Immunohistochemistry-Paraffin: MxA/Mx1 Antibody (HL2051) - Azide and BSA Free [NBP3-25572] -
Immunohistochemistry-Paraffin: MxA/Mx1 Antibody (HL2051) - Azide and BSA Free [NBP3-25572] - MX1 antibody [HL2051] detects MX1 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. MX1 stained by MX1 antibody [HL2051] (NBP3-25572) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: MxA/Mx1 Antibody (HL2051) - Azide and BSA Free [NBP3-25572] -
Western Blot: MxA/Mx1 Antibody (HL2051) - Azide and BSA Free [NBP3-25572] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MX1 antibody [HL2051] (NBP3-25572) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
Immunohistochemistry-Paraffin: MxA/Mx1 Antibody (HL2051) - Azide and BSA Free [NBP3-25572] -
Immunohistochemistry-Paraffin: MxA/Mx1 Antibody (HL2051) - Azide and BSA Free [NBP3-25572] - MX1 antibody [HL2051] detects MX1 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded cat kidney. MX1 stained by MX1 antibody [HL2051] (NBP3-25572) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: MxA/Mx1 Antibody (HL2051) - Azide and BSA Free [NBP3-25572] -
Western Blot: MxA/Mx1 Antibody (HL2051) - Azide and BSA Free [NBP3-25572] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MX1 antibody [HL2051] (NBP3-25572) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: MxA/Mx1 Antibody (HL2051) - Azide and BSA Free [NBP3-25572] -
Western Blot: MxA/Mx1 Antibody (HL2051) - Azide and BSA Free [NBP3-25572] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with MX1 antibody [HL2051] (NBP3-25572) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunohistochemistry-Paraffin: MxA/Mx1 Antibody (HL2051) - Azide and BSA Free [NBP3-25572] -
Immunohistochemistry-Paraffin: MxA/Mx1 Antibody (HL2051) - Azide and BSA Free [NBP3-25572] - MX1 antibody [HL2051] detects MX1 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. MX1 stained by MX1 antibody [HL2051] (NBP3-25572) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
MxA/Mx1 Antibody (HL2051) -
MX1 antibody [HL2051] detects MX1 protein by immunofluorescent analysis.Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: MX1 stained by MX1 antibody [HL2051] diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] diluted at 1:1000.Blue: Fluoroshield with DAPI.Applications for MxA/Mx1 Antibody (HL2051) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Assay dependent
Immunohistochemistry
Assay dependent
Immunohistochemistry-Paraffin
Assay dependent
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: MxA/Mx1
Long Name
Interferon-regulated Resistance GTP-binding protein MxA
Alternate Names
IFI-78K, IFI78, Mx1
Gene Symbol
MX1
Additional MxA/Mx1 Products
Product Documents for MxA/Mx1 Antibody (HL2051) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for MxA/Mx1 Antibody (HL2051) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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