MyD88 Antibody (OTI2B2) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-72842

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI2B2

Format

Azide and BSA Free
View all MyD88 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human MYD88 (NP_002459) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

33.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MyD88 Antibody (OTI2B2) - Azide and BSA Free

Western Blot: MyD88 Antibody (OTI2B2)Azide and BSA Free [NBP2-72842]

Western Blot: MyD88 Antibody (OTI2B2)Azide and BSA Free [NBP2-72842]

Western Blot: MyD88 Antibody (OTI2B2) - Azide and BSA Free [NBP2-72842] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MyD88 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MyD88.
Immunocytochemistry/ Immunofluorescence: MyD88 Antibody (OTI2B2) - Azide and BSA Free [NBP2-72842]

Immunocytochemistry/ Immunofluorescence: MyD88 Antibody (OTI2B2) - Azide and BSA Free [NBP2-72842]

Immunocytochemistry/Immunofluorescence: MyD88 Antibody (OTI2B2) - Azide and BSA Free [NBP2-72842] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY MyD88.
Immunohistochemistry: MyD88 Antibody (OTI2B2) - Azide and BSA Free [NBP2-72842]

Immunohistochemistry: MyD88 Antibody (OTI2B2) - Azide and BSA Free [NBP2-72842]

Immunohistochemistry: MyD88 Antibody (OTI2B2) - Azide and BSA Free [NBP2-72842] - Staining of paraffin-embedded Human prostate tissue using anti-MyD88 mouse monoclonal antibody.
Flow Cytometry: MyD88 Antibody (OTI2B2) - Azide and BSA Free [NBP2-72842]

Flow Cytometry: MyD88 Antibody (OTI2B2) - Azide and BSA Free [NBP2-72842]

Flow Cytometry: MyD88 Antibody (OTI2B2) - Azide and BSA Free [NBP2-72842] - Analysis of Jurkat cells, using antiMYD88 antibody, (Red), compared to a nonspecific negative control antibody, (Blue).
Immunohistochemistry: MyD88 Antibody (OTI2B2) - Azide and BSA Free [NBP2-72842]

Immunohistochemistry: MyD88 Antibody (OTI2B2) - Azide and BSA Free [NBP2-72842]

Immunohistochemistry: MyD88 Antibody (OTI2B2) - Azide and BSA Free [NBP2-72842] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-MyD88 mouse monoclonal antibody.
Immunohistochemistry: MyD88 Antibody (OTI2B2) - Azide and BSA Free [NBP2-72842]

Immunohistochemistry: MyD88 Antibody (OTI2B2) - Azide and BSA Free [NBP2-72842]

Immunohistochemistry: MyD88 Antibody (OTI2B2) - Azide and BSA Free [NBP2-72842] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-MyD88 mouse monoclonal antibody.
Immunohistochemistry: MyD88 Antibody (OTI2B2) - Azide and BSA Free [NBP2-72842]

Immunohistochemistry: MyD88 Antibody (OTI2B2) - Azide and BSA Free [NBP2-72842]

Immunohistochemistry: MyD88 Antibody (OTI2B2) - Azide and BSA Free [NBP2-72842] - Staining of paraffin-embedded Human Kidney tissue using anti-MyD88 mouse monoclonal antibody.
Immunohistochemistry: MyD88 Antibody (OTI2B2) - Azide and BSA Free [NBP2-72842]

Immunohistochemistry: MyD88 Antibody (OTI2B2) - Azide and BSA Free [NBP2-72842]

Immunohistochemistry: MyD88 Antibody (OTI2B2) - Azide and BSA Free [NBP2-72842] - Staining of paraffin-embedded Human lymph node tissue using anti-MyD88 mouse monoclonal antibody.
Immunohistochemistry: MyD88 Antibody (OTI2B2) - Azide and BSA Free [NBP2-72842]

Immunohistochemistry: MyD88 Antibody (OTI2B2) - Azide and BSA Free [NBP2-72842]

Immunohistochemistry: MyD88 Antibody (OTI2B2) - Azide and BSA Free [NBP2-72842] - Staining of paraffin-embedded Human thyroid tissue using anti-MyD88 mouse monoclonal antibody.
Flow Cytometry: MyD88 Antibody (OTI2B2) - Azide and BSA Free [NBP2-72842]

Flow Cytometry: MyD88 Antibody (OTI2B2) - Azide and BSA Free [NBP2-72842]

Flow Cytometry: MyD88 Antibody (OTI2B2) - Azide and BSA Free [NBP2-72842] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-MyD88 antibody, and then analyzed by flow cytometry.

Applications for MyD88 Antibody (OTI2B2) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:2000

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MyD88

Myeloid differentiation Marker 88 (MyD88) is a universal adaptor molecule for the Toll/IL1R and is involved in the inflammatory response induced by IL1, IL18 and LPS. MyD88 associates with and recruits IRAK to IL1 receptor complex in response to IL1. This pathway further leads to activation of NFkB. Targeted disruption of the MyD88 gene results in lose of cellular responses to IL1 and IL18, and MyD88 deficient mice lack responses to bacterial product LPS that employs Toll like receptors 2 and 4 as the signaling receptors.

Long Name

Myeloid Differentiation Primary Response Gene 88

Alternate Names

MYD88D, myeloid differentiation primary response gene (88), myeloid differentiation primary response protein MyD88

Gene Symbol

MYD88

Additional MyD88 Products

Product Documents for MyD88 Antibody (OTI2B2) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for MyD88 Antibody (OTI2B2) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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