MyD88 Inhibitor Peptide Set

Novus Biologicals | Catalog # NBP2-29328

Novus Biologicals
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Key Product Details

Species

Human, Mouse, Rat, Bovine, Xenopus, Yeast, Zebrafish

Applications

Block/Neutralize, ELISA, Flow Cytometry, Functional Assay, Immunohistochemistry, Inhibition Activity

Product Specifications

Specificity

The MyD88 Homodimerization inhibitory peptide contains a protein transduction (PTD) sequence (DRQIKIWFQNRRMKWKK) derived from antennapedia which renders the peptide cell permeable (Derossi et al, The third helix of the antennapedia homeodomain translocates through biological membranes. J Biol Chem. 269:10444-10450 (1994)].The control peptide consists of only the PTD sequence.

Applications

Immunohistochemistry (reported in scientific literature (PMID 28282921))
ELISA (1:100 - 1:2000. Use reported in scientific literature (PMID 23469306))
Binding Inhibition (reported in scientific literature (PMID 28302163))
Block/Neutralize (reported in scientific literature (PMID 24886588))
Flow Cytometry (reported in scientific literature (PMID 27870876))
In vitro assay (reported in scientific literature (PMID 25810567))
In vivo assay (reported in scientific literature (PMID 24755282))
Functional (Inhibition) (reported in multiple pieces of scientific literature)

Application Notes

Inhibition of MyD88 dependent TLR/IL-1R signaling activitiy by interefering with MyD88 homodimer formation.

Inhibitory peptide at 100 uM concentration may be a starting point. However, useful concentration of peptide may vary depending on experimental condition and cell type. Incubate cells for 24 hr with peptides before stimulating with ligands.

Reactivity Notes

Yeast reactivity reported in scientific literature (PMID: 27870876).

Inhibitor Content

2 x 1.0mg: MyD88 Homodimerization Inhibitor peptide (lyophilized) DRQIKIWFQNRRMKWKKRDVLPGT (MyD88 homodimerization sequence: RDVLPGT). Molecular weight: 3100
2 x 1.0mg: Antennapedia Control peptide (lyophilized) DRQIKIWFQNRRMKWKK. Molecular weight: 2361
5mg size will contain 5mg of each peptide (MyD88 Homodimerization Inhibitor peptide and Antennapedia Control peptide).

Formulation, Preparation, and Storage

Preparation Method

Preparation of 5 mM Stock Solutions
PBS* is added directly to the vials to prepare the stock solutions. Note: Bring the solution to room temperature and quick spin the tubes before opening the caps.
MyD88 Homodimerization Inhibitor Peptide: 1 mg of DRQIKIWFQNRRMKWKKRDVLPGT
Add 64.4 ul of PBS* to the vial to make a 5 mM stock solution. Mix by vortexing. Aliquot and store at -20C or -80C. Avoid repeated freeze thawing.

Control Peptide: 1 mg of DRQIKIWFQNRRMKWKK
Add 84.8 ul PBS* to the vial. Mix by vortexing. Aliquot and store at -20C or -80C. Avoid repeated freeze thawing
*Recipe for 1X PBS:
1. Dissolve the following in 800ml distilled H2O.
- 8g of NaCl
- 0.2g of KCl
- 1.44g of Na2HPO4
- 0.24g of KH2PO4
2. Adjust pH to 7.5 with HCl.
3. Adjust volume to 1L with additional distilled H2O.
4. Sterilize by autoclaving

Please contact technical support for detailed reconstitution instructions.

Formulation

Solubilize the peptides prior to use by making 5 mM PBS* stock solutions (please see Preparation of 5 mM Stock Solutions under Preparation Method).

Concentration

Lyoph

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MyD88

MyD88 is an adapter protein that links Toll-like receptors (TLRs) and Interleukin-1 receptors (IL-1rs) with downstream signaling molecules. Homodimerization of MyD88 is a critical step in the signaling process, and allows the recruitment and activation of the kinase IRAK. MyD88 exists as a homodimer when recruited to activated TLR/IL-1Rs. The inhibitor peptide contains a sequence from the MyD88 TIR homodimerization domain (Loiarro, et al.). 1 MyD88 monomer binds to this inhibitor peptide, there by blocking MyD88 homodimerization.

Long Name

Myeloid Differentiation Primary Response Gene 88

Alternate Names

MYD88D, myeloid differentiation primary response gene (88), myeloid differentiation primary response protein MyD88

Gene Symbol

MYD88

Additional MyD88 Products

Product Documents for MyD88 Inhibitor Peptide Set

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for MyD88 Inhibitor Peptide Set

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Inhibitors are guaranteed for 1 year from date of receipt.

Citations for MyD88 Inhibitor Peptide Set

Customer Reviews for MyD88 Inhibitor Peptide Set (3)

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Protocols

View specific protocols for MyD88 Inhibitor Peptide Set (NBP2-29328):


Hazard Information
Chemical Name: Non hazardous products
Chemical Formula: N/A
CAS Number: N/A
EEC-No: N/A

Hazard Identification
None
First Aid Measures
Eye Contact: None
Skin Contact: None
Inhalation: None
Ingestion: None

Accidental Release Measures
This product either does not contain hazardous constituents or the concentration of all chemical constituents are below the regulatory threshold limits described by Occupational Safety Health Administration Hazard Communication Standard 29 CFR 1910.1200 and the European Directive 91/155/EEC. 88/379/EEC, and 67/546/EEC.

Handling and Storage
Exposure Controls / Personal Protection
Other Precautions: None

Physical and Chemical Properties
Form: N/A
Color: N/A
Odor: N/A
Melting Point: N/A
Boiling Temperature: N/A
Density: N/A
Vapor Pressure: N/A
Solubility in Water: N/A
Flash Point: N/A
Explosion limits: N/A
Ignition Temperature: N/A

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for MyD88 Inhibitor Peptide Set

Showing  1 - 4 of 4 FAQs Showing All

  • Q: I have a question regarding the product NBP2-29328 and want to know how the inhibitory peptide passes through the cell wall?

    A: The MyD88 inhibitory peptide contains a protein transduction (PTD) sequence derived from antennapedia which renders the PTD sequence renders the peptide cell permeable. The antennapedia technology was first described by Derossi et al, The third helix of the Antennapedia homeodomain translocates through biological membranes and is used in a number of our inhibitory peptides. The MyD88 inhibitory peptide also contains a sequence from the MyD88 TIR homodimerization domain (Loiarro et al, Peptide-mediated interference of TIR domain dimerization in MyD88 inhibits interleukin-1-dependent activation of NF-{kappa}B). The control peptide consists of only the PTD sequence. The Derossi and Loiarro technology references are attached for additional background information. Please also follow this link to representative publications citing the MyD88 inhibitory peptide.

  • Q: I was wondering whether NBP2-29328-5mg has biological activity.

    A: Our MyD88 Inhibitor Peptide Set does have biological activity.

  • Q: I'm looking into purchasing NBP2-29328 for use and I am wondering if you can give me more information. Specifically, I would like to inject this drug ICM (directly into the CNS) using a rat model, and I am wondering what information you can provide about

    A: Unfortunately, I do not see any in-vivo data for this product on our internal records. Most of the information that we have on our records for this product may be seen at its online datasheets

  • Q: Is this the same as ST2825 compound described by Loiarro et al, 2007?

    A: ST2825 is the heptapeptide only, whereas the inhibitor peptide from NBP2-29328 corresponds to the association between the hexapeptide + the PTD sequence from antennapedia. In that case, this would suggest that the product used in Loiarro et al. is not the same as NBP2-29328.

  • Q: I have a question regarding the product NBP2-29328 and want to know how the inhibitory peptide passes through the cell wall?

    A: The MyD88 inhibitory peptide contains a protein transduction (PTD) sequence derived from antennapedia which renders the PTD sequence renders the peptide cell permeable. The antennapedia technology was first described by Derossi et al, The third helix of the Antennapedia homeodomain translocates through biological membranes and is used in a number of our inhibitory peptides. The MyD88 inhibitory peptide also contains a sequence from the MyD88 TIR homodimerization domain (Loiarro et al, Peptide-mediated interference of TIR domain dimerization in MyD88 inhibits interleukin-1-dependent activation of NF-{kappa}B). The control peptide consists of only the PTD sequence. The Derossi and Loiarro technology references are attached for additional background information. Please also follow this link to representative publications citing the MyD88 inhibitory peptide.

  • Q: I was wondering whether NBP2-29328-5mg has biological activity.

    A: Our MyD88 Inhibitor Peptide Set does have biological activity.

  • Q: I'm looking into purchasing NBP2-29328 for use and I am wondering if you can give me more information. Specifically, I would like to inject this drug ICM (directly into the CNS) using a rat model, and I am wondering what information you can provide about

    A: Unfortunately, I do not see any in-vivo data for this product on our internal records. Most of the information that we have on our records for this product may be seen at its online datasheets

  • Q: Is this the same as ST2825 compound described by Loiarro et al, 2007?

    A: ST2825 is the heptapeptide only, whereas the inhibitor peptide from NBP2-29328 corresponds to the association between the hexapeptide + the PTD sequence from antennapedia. In that case, this would suggest that the product used in Loiarro et al. is not the same as NBP2-29328.

  • Q: I have a question regarding the product NBP2-29328 and want to know how the inhibitory peptide passes through the cell wall?

    A: The MyD88 inhibitory peptide contains a protein transduction (PTD) sequence derived from antennapedia which renders the PTD sequence renders the peptide cell permeable. The antennapedia technology was first described by Derossi et al, The third helix of the Antennapedia homeodomain translocates through biological membranes and is used in a number of our inhibitory peptides. The MyD88 inhibitory peptide also contains a sequence from the MyD88 TIR homodimerization domain (Loiarro et al, Peptide-mediated interference of TIR domain dimerization in MyD88 inhibits interleukin-1-dependent activation of NF-{kappa}B). The control peptide consists of only the PTD sequence. The Derossi and Loiarro technology references are attached for additional background information. Please also follow this link to representative publications citing the MyD88 inhibitory peptide.

  • Q: I was wondering whether NBP2-29328-5mg has biological activity.

    A: Our MyD88 Inhibitor Peptide Set does have biological activity.

  • Q: I'm looking into purchasing NBP2-29328 for use and I am wondering if you can give me more information. Specifically, I would like to inject this drug ICM (directly into the CNS) using a rat model, and I am wondering what information you can provide about

    A: Unfortunately, I do not see any in-vivo data for this product on our internal records. Most of the information that we have on our records for this product may be seen at its online datasheets

  • Q: Is this the same as ST2825 compound described by Loiarro et al, 2007?

    A: ST2825 is the heptapeptide only, whereas the inhibitor peptide from NBP2-29328 corresponds to the association between the hexapeptide + the PTD sequence from antennapedia. In that case, this would suggest that the product used in Loiarro et al. is not the same as NBP2-29328.

  • Q: I have a question regarding the product NBP2-29328 and want to know how the inhibitory peptide passes through the cell wall?

    A: The MyD88 inhibitory peptide contains a protein transduction (PTD) sequence derived from antennapedia which renders the PTD sequence renders the peptide cell permeable. The antennapedia technology was first described by Derossi et al, The third helix of the Antennapedia homeodomain translocates through biological membranes and is used in a number of our inhibitory peptides. The MyD88 inhibitory peptide also contains a sequence from the MyD88 TIR homodimerization domain (Loiarro et al, Peptide-mediated interference of TIR domain dimerization in MyD88 inhibits interleukin-1-dependent activation of NF-{kappa}B). The control peptide consists of only the PTD sequence. The Derossi and Loiarro technology references are attached for additional background information. Please also follow this link to representative publications citing the MyD88 inhibitory peptide.

  • Q: I was wondering whether NBP2-29328-5mg has biological activity.

    A: Our MyD88 Inhibitor Peptide Set does have biological activity.

  • Q: I'm looking into purchasing NBP2-29328 for use and I am wondering if you can give me more information. Specifically, I would like to inject this drug ICM (directly into the CNS) using a rat model, and I am wondering what information you can provide about

    A: Unfortunately, I do not see any in-vivo data for this product on our internal records. Most of the information that we have on our records for this product may be seen at its online datasheets

  • Q: Is this the same as ST2825 compound described by Loiarro et al, 2007?

    A: ST2825 is the heptapeptide only, whereas the inhibitor peptide from NBP2-29328 corresponds to the association between the hexapeptide + the PTD sequence from antennapedia. In that case, this would suggest that the product used in Loiarro et al. is not the same as NBP2-29328.

Showing  1 - 4 of 4 FAQs Showing All