MYH6 Antibody (CL2148) - BSA Free

Novus Biologicals | Catalog # NBP2-36744

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (97%), Rat (96%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL2148

Format

BSA Free
View all MYH6 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from P13533, with the exact immunogen sequence remaining proprietary.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for MYH6 Antibody (CL2148) - BSA Free

Immunohistochemistry-Paraffin: MYH6 Antibody (CL2148) [NBP2-36744]

Immunohistochemistry-Paraffin: MYH6 Antibody (CL2148) [NBP2-36744]

Immunohistochemistry-Paraffin: MYH6 Antibody (CL2148) [NBP2-36744] - Staining in human heart muscle and liver tissues. Corresponding MYH6 RNA-seq data are presented for the same tissues.
Western Blot: MYH6 Antibody (CL2148) [NBP2-36744]

Western Blot: MYH6 Antibody (CL2148) [NBP2-36744]

Western Blot: MYH6 Antibody (CL2148) [NBP2-36744] - Analysis of human skeletal muscle tissue lysate.
Immunohistochemistry-Paraffin: MYH6 Antibody (CL2148) [NBP2-36744]

Immunohistochemistry-Paraffin: MYH6 Antibody (CL2148) [NBP2-36744]

Immunohistochemistry-Paraffin: MYH6 Antibody (CL2148) [NBP2-36744] - Staining of human heart muscle shows very strong cytoplasmic positivity in cardiomyocytes.
Immunohistochemistry-Paraffin: MYH6 Antibody (CL2148) [NBP2-36744]

Immunohistochemistry-Paraffin: MYH6 Antibody (CL2148) [NBP2-36744]

Immunohistochemistry-Paraffin: MYH6 Antibody (CL2148) [NBP2-36744] - Staining of human skeletal muscle shows strong cytoplasmic positivity in striated muscle fibers.
Immunohistochemistry-Paraffin: MYH6 Antibody (CL2148) [NBP2-36744]

Immunohistochemistry-Paraffin: MYH6 Antibody (CL2148) [NBP2-36744]

Immunohistochemistry-Paraffin: MYH6 Antibody (CL2148) [NBP2-36744] - Staining of human liver shows no positivity in hepatocytes as expected.

Applications for MYH6 Antibody (CL2148) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:5000 - 1:10000

Immunohistochemistry-Paraffin

1:5000 - 1:10000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Reviewed Applications

Read 1 review rated 4 using NBP2-36744 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MYH6

Skeletal muscle Myosin or Myosin II is the motor protein that generates force to drive muscle contraction. It is a 520 kDa hexamer comprised of two heavy chains and four light chains. Myosin heavy chain is 220 kDa in size and consists of a long coiled-coil domain tail that mediates dimerization of the two heavy chains and a globular head region that mediates ATP-dependent sliding of actin filaments. Myosin heavy chain can be proteolytically cleaved to produce heavy meromyosin, which includes the S1 motor domain (head region) and first third of the coiled coil domain, and light meromyosin, which includes the C-terminal two thirds of the coiled coil domain.

Long Name

Myosin Heavy Chain 6, Cardiac Muscle, Alpha

Alternate Names

alpha-MHC, ASD3, CMD1EE, CMH14, MyHC-Alpha, MYHCA, Myosin-6, SSS3

Gene Symbol

MYH6

UniProt

P13533

Additional MYH6 Products

Product Documents for MYH6 Antibody (CL2148) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for MYH6 Antibody (CL2148) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cardiac Stem Cell Markers

Customer Reviews for MYH6 Antibody (CL2148) - BSA Free (1)

4 out of 5
1 Customer Rating
5 Stars
0%
4 Stars
100%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used MYH6 Antibody (CL2148) - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 1 of 1 review Showing All
Filter By:
Clear Filters X
  • MYH6 Antibody (CL2148)
    Name: anita quon
    Application: Western Blot
    Sample Tested: Adult heart
    Species: Rat
    Verified Customer | Posted 09/06/2019
    Adult rat heart lysates, reduced and denatured, 200 ug total protein resolved on 5% SDS-acrylamide gel. Transfer onto 0.22 um nitrocellulose membrane. Block with Rockland blocking solution. incubate membrane overnight at 4C with 1:75 dilution of MYH6 antibody. wash with PBS-0.1% tween 20. incubate for one hour at room temp with 1:10 000 LiCor IRDye 800CW donkey anti-mouse IgG. Images taken with Odyssey V3.0.
    MYH6 Antibody (CL2148) - BSA Free NBP2-36744

There are no reviews that match your criteria.

Showing  1 - 1 of 1 review Showing All

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies