MYL4 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-30954

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all MYL4 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: PAPEAPKEPAFDPKSVKIDFTADQIE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MYL4 Antibody - BSA Free

Immunohistochemistry-Paraffin: MYL4 Antibody [NBP2-30954]

Immunohistochemistry-Paraffin: MYL4 Antibody [NBP2-30954]

Immunohistochemistry-Paraffin: MYL4 Antibody [NBP2-30954] - Staining in human heart muscle and skeletal muscle tissues using anti-MYL4 antibody. Corresponding MYL4 RNA-seq data are presented for the same tissues.
Immunocytochemistry/ Immunofluorescence: MYL4 Antibody [NBP2-30954]

Immunocytochemistry/ Immunofluorescence: MYL4 Antibody [NBP2-30954]

Immunocytochemistry/Immunofluorescence: MYL4 Antibody [NBP2-30954] - Staining of human cell line U-2 OS shows localization to plasma membrane. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: MYL4 Antibody [NBP2-30954]

Immunohistochemistry-Paraffin: MYL4 Antibody [NBP2-30954]

Immunohistochemistry-Paraffin: MYL4 Antibody [NBP2-30954] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
Immunohistochemistry-Paraffin: MYL4 Antibody [NBP2-30954]

Immunohistochemistry-Paraffin: MYL4 Antibody [NBP2-30954]

Immunohistochemistry-Paraffin: MYL4 Antibody [NBP2-30954] - Staining of human cerebral cortex shows no positivity in neurons as expected.
Immunohistochemistry-Paraffin: MYL4 Antibody [NBP2-30954]

Immunohistochemistry-Paraffin: MYL4 Antibody [NBP2-30954]

Immunohistochemistry-Paraffin: MYL4 Antibody [NBP2-30954] - Staining of human colon shows no positivity in glandular cells as expected.
Immunohistochemistry-Paraffin: MYL4 Antibody [NBP2-30954]

Immunohistochemistry-Paraffin: MYL4 Antibody [NBP2-30954]

Immunohistochemistry-Paraffin: MYL4 Antibody [NBP2-30954] - Staining of human heart muscle shows strong cytoplasmic positivity in myocytes.
Simple Western: MYL4 Antibody [NBP2-30954]

Simple Western: MYL4 Antibody [NBP2-30954]

Simple Western: MYL4 Antibody [NBP2-30954] - Simple Western lane view shows a specific band for MYL4 in 0.2 mg/ml of HEL (left) and h. Heart (right) lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Simple Western: MYL4 Antibody [NBP2-30954]

Simple Western: MYL4 Antibody [NBP2-30954]

Simple Western: MYL4 Antibody [NBP2-30954] - Electropherogram image of the corresponding Simple Western lane view. MYL4 antibody was used at 1:50 dilution on HEL and h. Heart lysate(s) respectively.
MYL4 Antibody

Western Blot: MYL4 Antibody [NBP2-30954] -

Western Blot: MYL4 Antibody [NBP2-30954] -Analysis in human cell line HEL.

Applications for MYL4 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Simple Western

1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
See Simple Western Antibody Database for Simple Western validation: Tested in HEL and h. Heart lysates, separated by Size, antibody dilution of 1:50, apparent MW was 33 kDa

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MYL4

Myosin is a hexameric ATPase cellular motor protein. It is composed of two myosin heavy chains, two nonphosphorylatable myosin alkali light chains, and two phosphorylatable myosin regulatory light chains. This gene encodes a myosin alkali light chain that is found in embryonic muscle and adult atria. Two alternatively spliced transcript variants encoding the same protein have been found for this gene. (provided by RefSeq)

Alternate Names

ALC1, AMLC, GT1, MLC1, Myosin light chain 1, embryonic muscle/atrial isoform, myosin light chain 4, Myosin light chain alkali GT-1 isoform, myosin, atrial/fetal muscle, light chain, myosin, light chain 4, alkali; atrial, embryonic, myosin, light polypeptide 4, alkali; atrial, embryonic, PRO1957

Gene Symbol

MYL4

UniProt

P12829 (Human)

Additional MYL4 Products

Product Documents for MYL4 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for MYL4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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